%PDF-1.3 % 1 0 obj << /Type /Pages /Count 6 /Kids [ 3 0 R 4 0 R 5 0 R 6 0 R 7 0 R 8 0 R ] >> endobj 2 0 obj << /Producer (PyPDF2) /Title (Folding\040DNA\040to\040create\040nanoscale\040shapes\040and\040patterns) /Author (Paul\040W\056\040K\056\040Rothemund) /Subject (\047Bottom\055up\040fabrication\047\054\040which\040exploits\040the\040intrinsic\040properties\040of\040atoms\040and\040molecules\040to\040direct\040their\040self\055organization\054\040is\040widely\040used\040to\040make\040relatively\040simple\040nanostructures\056\040A\040key\040goal\040for\040this\040approach\040is\040to\040create\040nanostructures\040of\040high\040complexity\054\040matching\040that\040routinely\040achieved\040by\040\047top\055down\047\040methods\056\040The\040self\055assembly\040of\040DNA\040molecules\040provides\040an\040attractive\040route\040towards\040this\040goal\056\040Here\040I\040describe\040a\040simple\040method\040for\040folding\040long\054\040single\055stranded\040DNA\040molecules\040into\040arbitrary\040two\055dimensional\040shapes\056\040The\040design\040for\040a\040desired\040shape\040is\040made\040by\040raster\055filling\040the\040shape\040with\040a\0407\055kilobase\040single\055stranded\040scaffold\040and\040by\040choosing\040over\040200\040short\040oligonucleotide\040\047staple\040strands\047\040to\040hold\040the\040scaffold\040in\040place\056\040Once\040synthesized\040and\040mixed\054\040the\040staple\040and\040scaffold\040strands\040self\055assemble\040in\040a\040single\040step\056\040The\040resulting\040DNA\040structures\040are\040roughly\040100\040nm\040in\040diameter\040and\040approximate\040desired\040shapes\040such\040as\040squares\054\040disks\040and\040five\055pointed\040stars\040with\040a\040spatial\040resolution\040of\0406\040nm\056\040Because\040each\040oligonucleotide\040can\040serve\040as\040a\0406\055nm\040pixel\054\040the\040structures\040can\040be\040programmed\040to\040bear\040complex\040patterns\040such\040as\040words\040and\040images\040on\040their\040surfaces\056\040Finally\054\040individual\040DNA\040structures\040can\040be\040programmed\040to\040form\040larger\040assemblies\054\040including\040extended\040periodic\040lattices\040and\040a\040hexamer\040of\040triangles\040\050which\040constitutes\040a\04030\055megadalton\040molecular\040complex\051\056) /Keywords (16\040Mar\0402006\054\040Nature\054\040DNA\040origami\054\040DNA\040nanotechnology\054\040California\040Institute\040of\040Technology) >> endobj 3 0 obj << /CropBox [ 0 0 612 792 ] /Parent 1 0 R /Contents [ 10 0 R 11 0 R 12 0 R 13 0 R 14 0 R 15 0 R 16 0 R 17 0 R ] /Rotate 0 /MediaBox [ 0 0 612 792 ] /Resources 18 0 R /Type /Page >> endobj 4 0 obj << /CropBox [ 0 0 612 792 ] /Parent 1 0 R /Contents 60 0 R /Rotate 0 /MediaBox [ 0 0 612 792 ] /Resources 67 0 R /Type /Page >> endobj 5 0 obj << /CropBox [ 0 0 612 792 ] /Parent 1 0 R /Contents 75 0 R /Rotate 0 /MediaBox [ 0 0 612 792 ] /Resources 82 0 R /Type /Page >> endobj 6 0 obj << /CropBox [ 0 0 612 792 ] /Parent 1 0 R /Contents 97 0 R /Rotate 0 /MediaBox [ 0 0 612 792 ] /Resources 104 0 R /Type /Page >> endobj 7 0 obj << /CropBox [ 0 0 612 792 ] /Parent 1 0 R /Contents 116 0 R /Rotate 0 /MediaBox [ 0 0 612 792 ] /Resources 123 0 R /Type /Page >> endobj 8 0 obj << /CropBox [ 0 0 612 792 ] /Parent 1 0 R /Contents 127 0 R /Rotate 0 /MediaBox [ 0 0 612 792 ] /Resources 134 0 R /Type /Page >> endobj 9 0 obj << /Type /Catalog /Pages 1 0 R >> endobj 10 0 obj << /Filter /FlateDecode /Length 1531 >> stream H|Vێ6}Gz5Wԍc.ijmmEUֻ;Ñd)49sЫVtAlV"0Uok[Μtߙ2-vu 乔Qݯ$/r/$E B3Jz7m@`y̞tZ|,<CX}i,,Zol!/n$oݾq㯗< k 2|&Y`,S}Cî6W'|"MFs}p^v^P*xp}C;1i3!1QEg9Mp)@fl1*X A(;AE|Q8L"B12ǴWI4A7wLsXo"y1-~ .XikmTc]֛~+1sGj;C/X^w FJB^B˩4Oy({5ԐXgN{`> pe);, 1 6S4< E 鑵"}aK(Ee>j\ΘqV/lzmaܫXx 0E |! ;My/0L?5=%pMu3M߭7L9vMF2ٽg)9Ə.b2r)*{t[ LG LE91mnY_aϦW QY5b'IS1p 2O% X:sjQR=r\ %ZCQ'4H;XURánh-jj;239#y2 ؏o`&O,u KpBX+-j` @~ fpQMFzӌba<*x)ңr9dGh+":%ab۽"<3Sӎk|).m@`vTd];UR}z%):%ٓf,I|jTo~$ Z3;Hګ-f:7J6 ՚ȋQ0|巨GZ#