Can we differentiate between M tuberculosis and M bovis by AFB staining?
Answers from top 6 papers
More filters
Papers (6) | Insight |
---|---|
87 Citations | This technique provides a simple and extremely sensitive method of identifying isolates of M. bovis and other pathogenic M. tuberculosis complex organisms. |
Because of its significantly different epidemiologic profile, compared with that of M. tuberculosis TB, we recommend routine surveillance of M. bovis TB. | |
16 Citations | This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species. |
28 Citations | Moreover they emphasise that human tuberculosis caused by M bovis persists and might be an underappreciated cause of morbidity and mortality. |
41 Citations | Thus, DR and pMBA2 could be, at the moment, the probes of choice for comparisons of M. bovis isolates in different regions and for epidemiological surveillance of bovine tuberculosis. |
93 Citations | Because of the phylogenetic proximity of the strains, even if most of these markers have been discovered in M. tuberculosis, they could be successfully adapted to the other bacteria of the M. tuberculosis complex, especially M. bovis. |
Related Questions
What are the current gap and limitations in using AFB for TB diagnosis?5 answersCurrent gaps and limitations in using Acid-Fast Bacilli (AFB) for tuberculosis (TB) diagnosis include low sensitivity, labor intensity, and challenges in specific patient populations. A study highlighted the limitations of AFB staining, emphasizing its low sensitivity and labor-intensive nature. Additionally, traditional TB diagnosis methods like sputum smear microscopy have limitations in HIV-positive patients, children, and latent TB infections. Diagnostic gaps in TB care cascade were identified, including limited access to diagnostic tests, high risk of missed diagnosis, and failure to diagnose despite reaching healthcare facilities. While AFB microscopy is specific, its sensitivity ranges from 35-70%, necessitating the exploration of alternative methods like Immuno Chromatography Test (ICT) for improved TB diagnosis. Efforts to enhance AFB detection through image segmentation techniques have been made to address challenges like over- and under-segmentation in TB diagnosis.
What are the best tuberculosis molecular tests?5 answersThe best tuberculosis molecular tests include the GeneXpert system, Xpert MTB/RIF, Line-probe assays (LPAs), and the CapitalBio Mycobacterium real-time polymerase chain reaction assay. GeneXpert technology, utilizing cartridge systems, offers high sensitivity, rapid results, and drug resistance detection. Xpert MTB/RIF and LPAs demonstrate accuracy in detecting rifampicin resistance. The CapitalBio test, while slightly less accurate than Xpert MTB/RIF, shows promise in diagnosing tuberculous lymphadenitis. These molecular tests play a crucial role in detecting TB and drug resistance, aiding in timely and accurate diagnosis for effective treatment. Each test has its strengths, with GeneXpert being particularly advantageous for its speed and sensitivity, while LPAs and Xpert MTB/RIF excel in detecting rifampicin resistance.
What is the laboratory type for mycobacterium tuberculosis?3 answersThe laboratory type for Mycobacterium tuberculosis (Mtb) includes various methods for detection and identification. These methods include stain and culture, which are traditionally followed by phenotypic-based identification methods. However, molecular methods are now considered the gold standard for the rapid detection of Mtb directly from patient specimens and for the identification of Mtb following growth in culture. Molecular methods include DNA probes, broad-range PCR with DNA sequencing, and next-generation sequencing, which provides identification, susceptibility, and typing information simultaneously. In addition to detection and identification, laboratory testing for Mtb drug resistance is also important. This can be done using methods such as antimicrobial susceptibility testing (AST) and sequencing of resistant cultures. Overall, the laboratory plays a critical role in the diagnosis of Mtb, utilizing a combination of traditional and molecular methods for detection, identification, and drug resistance testing.
What stain is used for Mycobacterium tuberculosis?7 answers
Which stain is used for tuberculosis?4 answers
Which staining technique is used in the diagnosis of tuberculosis and leprosy?3 answers