scispace - formally typeset
Search or ask a question

How does stem cell culture work? 


Best insight from top research papers

Stem cell culture involves the cultivation and maintenance of stem cells in a controlled environment. Stem cells are undifferentiated cells that have the ability to self-renew and differentiate into specialized cell types. They can be obtained from various sources, including adult tissues and organs. Stem cell culture systems have been developed to provide an optimal environment for the growth and proliferation of stem cells. These systems often include specialized culture media, stent materials, and culture devices. The culture media may be serum-free and contain specific nutrients and growth factors to support stem cell growth and differentiation . Stent materials, such as chitosan and gelatin frameworks, can provide a scaffold for stem cell attachment and enhance cell adsorption . Culture devices, such as stem cell culture robots, can facilitate the pretreatment and culture of biological tissues, improving the efficiency and quality of stem cell culture . Overall, stem cell culture aims to provide an environment that mimics the natural microenvironment of stem cells, allowing for their expansion and differentiation in vitro .

Answers from top 5 papers

More filters
Papers (5)Insight
The paper does not provide a direct answer to the question of how stem cell culture works. It focuses on the development of cell culture substrates for stem cell culture and regenerative medicine.
The provided paper does not explain how stem cell culture works. It only describes a stem cell culture robot that can pretreat biological tissues and culture stem cells.
Patent
Wang Zheng, Wu Zhenhua 
25 Jan 2017
3 Citations
The provided paper does not explain how stem cell culture works. It focuses on a specific stem cell culture system using a serum-free culture medium and a stent material.
Book ChapterDOI
01 Jan 2023
The provided paper does not provide information on how stem cell culture works. The paper discusses the properties and advantages of adult stem cells but does not explain the process of stem cell culture.
Patent
28 Feb 2020
1 Citations
The provided paper is about a stem cell culture device, but it does not provide information on how stem cell culture works.

Related Questions

What is Stem cell therapy?3 answersStem cell therapy is a multidisciplinary field of medicine that involves the introduction of new adult stem cells into damaged tissue to treat disease or injury. It has potential applications in various conditions such as Alzheimer's disease, Parkinson's disease, spinal cord injury, heart disease, severe burns, and diabetes. Stem cells can repair or replace damaged neurons, organs, and tissues. They are also used in basic research to understand human development, cancer, and gene control, as well as to test new drugs. The regulation of stem cell therapy is crucial for patient safety and quality healthcare, involving governing bodies such as the World Health Organization and governmental regulatory bodies. Stem cell therapy facilities must adhere to structural and technical regulations, including good tissue practice and good manufacturing practice, equipment calibration, skilled personnel, standard operating procedures, and quality control systems. Stem cell therapy shows promise in the treatment of cancer by providing targeted drug delivery and enhancing tumor volume reduction and survival rates. It is also being explored as a potential treatment for central nervous system disorders such as epilepsy and post-traumatic stress disorder.
How does artificial intelligence work?5 answersArtificial intelligence (AI) is the ability of computers to perform tasks typically associated with intelligent beings. It involves machine learning and deep learning, which are applications of AI. AI technologies are designed to imitate human cognitive abilities, enabling computers to deal with complex and ill-defined problems in an intelligent and adaptive manner. In manufacturing, AI is used to increase productivity, reduce errors, and improve efficiency. It can detect defects in production lines, ensure correct configuration of parts, and optimize workflows. AI also has applications in journalism, where it can be used to find hidden patterns in large volumes of data, reducing the cost of investigative journalism. Municipalities in the Nordic regions are adopting AI for various purposes, such as using chatbots to answer citizen questions and predicting leaks in water and sewage networks.
How does sociology work?3 answersSociology is a social science that focuses on society, human social behavior, patterns of social relationships, social interaction, and aspects of culture associated with everyday life. It uses various methods of empirical investigation and critical analysis to develop a body of knowledge about social order and social change. Sociology seeks to fit observations of social phenomena into different conceptual systems, tracing its origins from Enlightenment thought to subsequent developments in various sociological thinkers. It also examines the array of concepts and methods of research that have been applied to the study of society. Sociology offers a more rigorous understanding of the social and can help dismantle commonly shared preconceived ideas about the social order. It provides valuable insights into the social institutions and processes that shape human behaviors, histories, and opportunities. Sociology teaches students how to use empirical data to understand current social realities and act effectively on the central issues of our time. Sociology studies human society from the smallest of social interactions to the emerging global society, focusing on interpersonal, societal, and global scales.
What are the sources of stem cells?3 answersStem cells can be obtained from various sources such as bone marrow, adipose tissue, Wharton jelly, peripheral blood, cord blood, and tissue-specific stem cells. Hematopoietic stem cells, specifically, can be obtained from bone marrow, peripheral blood, or umbilical cord blood. Endothelial progenitors are present in bone marrow, adipose tissue, cord blood, and tissue. Mesenchymal stem cells can be procured from different tissue sources including umbilical cord, synovial tissue, breast milk, menstrual blood, adipose tissue, bone marrow, and dentine pulp. Perinatal stem cells, on the other hand, can be derived from perinatal tissues such as the placenta, amnion, amniotic fluid, umbilical cord blood, and Wharton's jelly. These various sources of stem cells offer different characteristics and potential therapeutic applications.
How the stem cell theraoy done?4 answersStem cell therapy involves the use of stem cells to repair tissue in various organs of the body. The method described in one study involves intraluminally applying stem cells through a natural body vessel or duct leading to the target site of the tissue to be repaired. Another study focuses on the preparation of stem cell spheres by applying a liquid crystal state material to the surface of a cell culture container and implanting stem cells onto this material. A different approach involves culturing stem cells by introducing them into an amnion epithelial cell feeder layer and culturing them in a culture solution. Stem cells can also be isolated from mammals with haemangioma and used in various therapeutic methods, including systemic or direct implantation into a subject or further in vitro culture and differentiation. Stem cell plasticity is another important aspect, where stem cells from different sources can contribute to the recovery of damaged organs.
Why are stem cells called so?5 answers

See what other people are reading

What role do extracellular vesicles play in neurological disorders?
5 answers
Extracellular vesicles (EVs) play a significant role in neurological disorders. They have been investigated as potential therapeutics for neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. EVs derived from cells that support and protect neurons, such as astrocytes and microglia, have been studied extensively. These EVs regulate the molecular differentiation of neurons and can influence migratory dynamics, potentially affecting the assembly of cortical circuits. Furthermore, EVs have the ability to cross the blood-brain barrier, allowing for the delivery of therapeutic molecules to the brain tissues and facilitating intercellular communication. Small EVs (sEVs) have shown promise as drug delivery systems for targeted delivery to the brain, with low toxicity, high stability, and high delivery efficiency. Overall, EVs have emerged as important players in the pathogenesis and potential treatment of neurological disorders.
What is a neurite outgrowth assay?
5 answers
A neurite outgrowth assay is a method used to assess the growth and development of neurites, which are the projections from neurons that allow them to communicate with other cells. It is an important process in neurodevelopment and is used to evaluate the effects of drugs and environmental chemicals on the nervous system. Several methods have been developed for conducting neurite outgrowth assays, including staining techniques and image-based analysis. These assays can be performed using induced pluripotent stem cell (iPSC)-derived human neurons labeled with green fluorescent protein (GFP). Software tools have also been developed to automate the analysis of neurite outgrowth, providing measurements of neurite length, number of neurites, and other morphological features. The assay involves plating dissociated neurons on various substrates and measuring the length of the longest neurite outgrowth.
Can scar tissue on the cornea be healed?
5 answers
Scar tissue on the cornea can be healed using various therapeutic approaches. Recent advancements in molecular therapeutics have focused on interventions such as exosomes, targeted gene therapy, microRNAs, recombinant viral vectors, histone deacetylase inhibitors, bioactive molecules, growth factors, and nanotechnology. Light, specifically laser or ultraviolet radiation, has also been explored as an effective cross-linking agent for biomaterials and tissues, including cornea healing and tissue regeneration. Additionally, tissue microparticles derived from the extracellular matrix (ECM) have shown promise in reducing inflammation and promoting tissue repair in the cornea. Furthermore, manipulating the hepatocyte growth factor (HGF)/c-Met signaling pathway has been investigated as a means to promote quality corneal healing. These advancements provide potential strategies for treating corneal scarring and restoring corneal transparency.
What is the best treatment for Type 1 Diabetes to lead to a cure?
5 answers
The best treatment for Type 1 Diabetes to lead to a cure is still under development and research. Various therapeutic options are being explored, including β-cell replacement therapy by islet transplantation, non-insulin adjunct therapy, gene and stem cell-based therapies, immunotherapy, and automated treatment with an artificial pancreas. Stem cell therapy using islet-like insulin-producing cells derived from human pluripotent stem cells also shows potential for allowing patients to withdraw from insulin therapy. However, challenges remain, such as protecting the cells from the immune system. Insulin injection therapy remains the current standard method for managing Type 1 Diabetes, but it is expensive, complicated, and insufficient. While islet transplantation has shown significant achievement, the lack of appropriate donors and high cost have led researchers to explore other alternatives. In summary, the best treatment for Type 1 Diabetes to lead to a cure is still being investigated, with various therapeutic options and stem cell therapy showing promise, but challenges and limitations exist.
Have allogeneic MSC bioinks been used for 3D bioprinting cartilage?
3 answers
Allogeneic MSC bioinks have been used for 3D bioprinting cartilage. Sang et al. developed a photo-cross-linked ECM bioink composed of modified proteins and polysaccharides, including gelatin methacrylate, hyaluronic acid methacrylate, and chondroitin sulfate methacrylate, which improved the basic properties, biological properties, and chondrogenesis of synovium-derived MSCs (SMSCs). Lafuente-Merchan et al. focused on the development of nanocellulose-alginate (NC-Alg) based bioinks for 3D bioprinting for cartilage regeneration, to which chondroitin sulfate (CS) and dermatan sulfate (DS) were added. The addition of CS and DS improved the characteristics of the bioink in terms of rheology and cell viability and functionality. Galarraga et al. developed an in situ crosslinking approach that allowed the printing of non-viscous, photocrosslinkable bioinks, using a norbornene-modified hyaluronic acid (NorHA) macromer as a representative bioink. The printing process was cytocompatible, with high cell viability and homogenous distribution of mesenchymal stromal cells (MSCs) observed throughout printed constructs. Lee et al. created a photocurable bioink by hybridizing gelatin methacryloyl (GelMA) and glycidyl-methacrylated HA (GMHA) for material extrusion 3D bioprinting in cartilage regeneration. This bioink demonstrated reliable mechanical properties, rheological properties, and printability, and provided an excellent microenvironment for chondrogenesis of tonsil-derived mesenchymal stem cells (TMSCs).
What are the current trends in mitochondrial disorders research?
5 answers
Current trends in mitochondrial disorders research include the development of small-molecule therapies to improve mitochondrial performance. Priority setting partnerships involving patients, carers, and clinicians have been established to identify the most pressing research priorities for primary mitochondrial disorders, resulting in a list of unanswered research questions. The use of induced pluripotent stem cells has provided a unique opportunity to create in vitro models that mimic the complex pathologies and phenotype associated with mitochondrial disorders. Scientometric research has identified hotspots in the study of mitochondrial dysfunction on bone metabolism and related diseases, including oxidative stress, aging, gene expression, and the pathogenesis of bone metabolism-related diseases. These trends highlight the need for safe and effective strategies to mitigate mitochondrial impairments, the importance of addressing unanswered research questions, and the potential of patient-specific in vitro models and scientometric analysis in advancing our understanding of mitochondrial disorders.
How does SA-β-gal activity change during the cell cycle?
3 answers
SA-β-gal activity is a well-recognized biomarker used to assess senescence in cells. It is detected using a chromogenic substrate, such as X-Gal, which releases a visible blue product when hydrolyzed by SA-β-gal. SA-β-gal activity is commonly detected at pH 6.0. The activity of SA-β-gal is often associated with cell senescence and is used as a marker for the detection of senescent cells. SA-β-gal activity is detected in lysosomes of senescent cells. The expression of SA-β-gal can be influenced by various factors, including DNA damage, proliferative status of the cells, and certain pathologies. SA-β-gal activity can be quantified using different methods, including manual counting and automatic analysis using Fiji-based macro extension. The detection of SA-β-gal activity can be used to screen for senotherapeutic drugs that target senescent cells.
How does SA-β-gal activity work?
4 answers
Senescence-associated beta-galactosidase (SA-β-GAL) is an enzyme that accumulates in the lysosomes of senescent cells and hydrolyzes β-galactosides. It is a well-recognized biomarker used to assess senescence in vivo and in cell culture. SA-β-GAL activity can be detected using a chromogenic substrate, such as 5-bromo-4-chloro-3-indoyl-β-d-galactopyranoside (X-Gal), which releases a visible blue product at pH 6.0. The detection of SA-β-GAL activity is commonly used to identify senescent cells and has been used to study senescence in various tissues, including zebrafish, intervertebral discs, and fibroblast cultures. The activity of SA-β-GAL can be quantified using manual counting or automated analysis tools, such as a Fiji-based macro extension, which provides faster and more accurate results compared to manual counting. SA-β-GAL activity is one of the best-characterized markers for the detection of senescent cells and is commonly used in screening for senotherapeutic drugs.
Can Cyanide increase protein in humans?
5 answers
Cyanide exposure can lead to an increase in protein levels in humans. In a study on human hepatoma cells, exposure to purified cylindrospermopsin (CYN), a cyanobacterial toxin, resulted in alterations in the expression of proteins involved in protein folding, energy metabolism, and cell signaling, indicating a potential alteration in glucose metabolism and cell proliferation. Additionally, occupational and dietary exposure to cyanide from cassava processing and ingestion of cassava-based diets led to an increase in urinary and serum thiocyanate levels, a cyanide metabolite, in workers, suggesting an increase in protein metabolism. These findings suggest that cyanide exposure may affect protein metabolism in humans.
What is the immune response produced by ECoG implantation?
5 answers
The immune response produced by ECoG implantation involves an inflammatory reaction and the activation of macrophages. Macrophages are resident immune cells in the cochlea and have been shown to phagocytize implant material. In animal models, macrophage populations increase with surgical stress and the introduction of a foreign body. In the case of cochlear implantation (CI), multiple populations of macrophages have been detected in the fibrous sheath surrounding the CI path and within fibrotic areas within the scala tympani and the scala vestibuli. The presence and distribution of CD68 and Iba1 expressing positive macrophages are altered in CI compared to the nonimplanted cochlea. These findings suggest that CI induces an immunological response in macrophages, which may play a role in implant failure or reduced hearing. Further studies are needed to evaluate the specific effects of these macrophages in CI patients.
What is Emetic activity in staphylococcal enterotoxin?
5 answers
Emetic activity in staphylococcal enterotoxin refers to the ability of the toxin to induce vomiting. Staphylococcus aureus produces staphylococcal enterotoxins (SEs) that have demonstrated emetic activity in various models. These SEs, such as SEA to SEE, SEG to SEI, and SER to SET, can cause toxin-mediated diseases and are implicated in the pathogenesis of allergic diseases. The emetic activity of SEs is characterized by rapid onset symptoms, including nausea and violent vomiting. The specific mechanisms by which SEs induce emesis are not fully understood. However, certain regions of the SE molecule, such as regions 21-50 and 81-100, have been identified as important for both superantigenic and emetic activities. These regions could be potential targets for therapeutic intervention against SE exposure.