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How long after membrane sweep did contractions start? 

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No evidence of spontaneous membrane healing was found after fetoscopic procedures, suggesting that the membrane defect normally persists until delivery.
Membrane fouling could be effectively controlled, as indicated by backwash period as long as 60 days.
This finding suggests that membrane proteins become correctly oriented already during or immediately after synthesis, as they are inserted into the membrane.
The long-term operation reveals that the membrane is more prone to fouling gradually after chemical cleaning.
However, the recovered flux after a long backwash of the membranes fouled with backpulsing is 20–40% lower than that of the membrane fouled without backpulsing, apparently due to greater adhesive internal fouling when the membrane surface is frequently exposed by rapid backpulsing.
Ten cycles at 40% of mean critical membrane tension--representative of mean physiologic contractions--did not affect strength and stiffness of fetal membranes but reduced the work to rupture, thus indicating that contractions might increase the risk of premature rupture of the membrane.
Here, we describe a previously unidentified permeabilization of the vacuolar membrane at the start of egress, preceding membrane rupture, suggesting a new initiation step in egress.
This phenomenon might be responsible for the negligible membrane permeability restoration after NaOH cleaning.
Chemical cleaning efficiency gradually decreased, indicating irreversible membrane fouling during long term operation.
After-contractions are considered to be the result of intracellular Ca overload due to increased membrane permeability to Ca.

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