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How many amino acids are there in casein? 

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The investigation showed that the ten esseltial amino acids, arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophane, and valine, occur in the free form, a fact which, chemically speaking, proves that this dialyzed casein digest is an adequate source for the nitrogen needs of the body.
Thus three amino acids of casein, Ala, Gly and especially Met, account for the enzyme adaptation of the urea cycle on a high casein diet.
Since with this method we can determine over 40 physiological amino acids with a very good resolution, trace amounts of amino acids can also be determined.
It was suggested that this acid casein has significantly more aromatic amino acids, as compared to arginine and lysine.
However, the beneficial effects of these amino acids could just as well be explained by the presence of an excess of other amino acids in casein (amino acid imbalance) as by an absolute shortage of glycine, arginine or alanine.
Amino acid composition of the bacteria, as in the case of casein, indicates a first limitation of sulfur-containing amino acids.
This absorption is due to the presence of aromatic amino acids in the acid-soluble casein fragments.
These results may indicate that coating amino acids with casein minimized variation in absorption rate of amino acids, which resulted in relatively simultaneous availability of all amino acids to the tissues for optimal protein synthesis.
Our data suggest that the occurrence of additional amino acids that are widely distributed and genetically encoded is unlikely.
These observations would Seem to indicate that the mixture of amino-acids used, as prepared by the acid hydrolysis of casein plus 2% tryptophane and added cystine, is efficiently utilized by the body, probably in the active synthesis of body protein.

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