scispace - formally typeset
Search or ask a question

How much time does cell cycle of yeast takes? 

Answers from top 10 papers

More filters
Papers (10)Insight
Here we show that in budding yeast, the ability of cells to grow changes during the cell cycle.
2 h. Thus the cell cycle of yeast can be divided into an expandable phase from cell division to the initiation of DNA synthesis, the length of which is dependent on growth rate and a constant phase from the initiation of DNA synthesis to cell division which takes a constant time independent of generation time.
These findings underscore and extend earlier conclusions that most of the G1 interval of the yeast cell cycle is simply a period of ongoing growth.
These observations are predicted if a yeast cell requires a critical size before a particular cell cycle event can be completed and that after completion of this event cell division occurs following a period of time independent of growth rate.
We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.
The characteristics and the time course of the yeast cell cycle were found to be strongly dependent on the physiological environment.
These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell.
These data reveal fundamental scaling relationships between the duration of eukaryotic cell cycle phases and rates of cell proliferation, point to the necessary role of Cln3p in these relationships in yeast, and provide a mechanistic basis linking Cln3p levels to proliferation rates and the scaling of G1 with doubling time.
Open accessJournal ArticleDOI
Xiaoyan Leng, Hans-Georg Müller 
01 Oct 2006-Biostatistics
34 Citations
The application to yeast cell-cycle data establishes a natural time order of genes that is in line with cell-cycle phases.
Here we present evidence for an independent cell-cycle oscillator in the budding yeast Saccharomyces cerevisiae.

Related Questions

Candida albicans growth curve4 answersCandida albicans growth curve was studied using growth curve methodology, which was found to be more accurate than the microbial sensitivity test for evaluating the occurrence of the paradoxical effect. The growth curve of Candida albicans was also investigated in the presence of cadmium, and it was found that the organism was resistant to this heavy metal at a concentration of 50 µg/ml. The growth rate of C. albicans was influenced by environmental conditions such as pH, temperature, and culture medium, with the fastest growth rate observed at 37°C in modified Sabouraud glucose broth medium with pH 7.4. Differences in growth kinetic parameters were found between different Candida species, with C. glabrata being the fastest growing species and C. parapsilosis showing the longest lag phase. Planktonic growth and biofilm formation of Candida haemulonii species complex were also studied, with all clinical isolates forming biofilm on polystyrene in a time-dependent manner.
How to DNA sequence a yeast?3 answersDNA sequencing of yeast can be done using various methods. One protocol involves preparing yeast DNA by digesting the cell wall and lysing the resulting spheroplasts with SDS. This method yields several micrograms of yeast DNA that can be cleaved by restriction enzymes and used as a template in polymerase chain reaction (PCR). Another method involves preparing a DNA sequencing library from yeast genomic DNA for use with the Illumina sequencing platform. This method utilizes specific reagents purchased largely from New England BioLabs, reducing the cost of library preparation. The shearing and size selection steps can be modified for different insert sizes. Additionally, there are simple protocols for preparing templates for direct sequencing of yeast mitochondrial DNA (mtDNA) using automatic DNA analyzers. These protocols yield sufficient quantity and quality of template DNA and can be used for re-sequencing of mtDNA for comparative analyses of yeast strains.
How yeast makes ATP?3 answersYeast produces ATP through the activity of the mitochondrial ATP synthase, a large multisubunit complex responsible for ATP synthesis. The enzyme complex consists of a water-soluble F1 sector and a membrane-embedded F0 sector. The F0 sector contains a ring of subunits that rotates in response to proton translocation, while the F1 sector contains the catalytic sites for ATP synthesis. The rotation of the F0 subunit ring drives conformational changes in the F1 sector, leading to ATP synthesis. The yeast ATP synthase is encoded by both the nuclear and mitochondrial genomes, with most subunits encoded by the nuclear genome and imported into mitochondria. Loss of respiration in yeast affects energy-intensive processes such as the maintenance of the plasma membrane proton gradient and nutrient import. The assembly of the yeast ATP synthase involves two separate but coordinated pathways that converge at the end stage.
How does the histone coverage of DNA change in yeast replicative ageing?5 answersDuring yeast replicative ageing, there is a decrease in histone coverage of DNA. Nucleosome occupancy across the genome decreases by 50% during replicative ageing, leading to a loss of histones. This loss of histones is accompanied by a transcriptional induction of all yeast genes. Specifically, genes that are normally repressed by promoter nucleosomes are most induced, indicating a preferential loss of nucleosomes from their promoters. Additionally, there is an increase in ubiquitylation of histone H2B at telomeric heterochromatin regions in replicatively aged cells. These changes in histone coverage and modifications are associated with cellular ageing and have implications for lifespan regulation.
Can you summarize the Cellular quiescence in budding yeast?3 answersCellular quiescence in budding yeast is a reversible state where cells temporarily exit the cell cycle. Quiescence is induced in response to nutrient starvation signals and involves large-scale remodeling of gene expression, organelles, and metabolism. The process of quiescence consists of three phases: initiation, maintenance, and exit. Quiescent cells are viable and play important roles in diseases such as cancer. Understanding cell quiescence is crucial for developing strategies to target quiescent cells. Single-cell approaches are necessary to address the heterogeneity among quiescent cells. Research on cellular quiescence in budding yeast has provided valuable insights into the molecular bases and transitions involved in quiescence.
How long does it take DNA replication to occur?5 answers

See what other people are reading

What are different shapes of bacteria?
4 answers
Bacteria exhibit a wide range of shapes, including round, rod-shaped, curved, spiral, and helical cells. The shape of bacteria is functionally important as it affects various biological functions such as nutrient acquisition, motility, dispersion, stress resistance, and interactions with other organisms. Different factors, including peptidoglycan and cytoskeleton-like proteins, can regulate and maintain bacterial shape. Genetic regulation, particularly involving the bacterial actin MreB and its interacting proteins, plays a crucial role in determining the shape of rod-shaped bacteria like Escherichia coli. Recent research has also shed light on the complex mechanisms underlying chromosome segregation and cell division in bacteria with spherical shapes, known as cocci. The molecular determinants underlying bacterial morphology have implications in niche adaptation, pathogenesis, and can potentially be targeted for antimicrobial strategies.
How does cell size regulates the trigger of cell division?
5 answers
Cell size regulates the trigger of cell division through various mechanisms. One mechanism involves the scaling of regulatory protein copy numbers with cell size, where cell-cycle activators tend to increase with cell size while inhibitors tend to decrease. Another mechanism involves the dilution of the cell cycle inhibitor Retinoblastoma protein (Rb) through cell growth in the G1 phase, which promotes cell cycle progression from G1 to S phase. This ensures that all cells at birth inherit a similar amount of Rb protein, independent of cell size. These mechanisms provide molecular mechanisms for cell size homeostasis and the coupling of cell growth to division. Additionally, titration mechanisms that involve the titration of proteins against DNA may also play a role in cell-size sensing and regulation.
How does the presence of pollutants affect the overall rate of biodegradation?
5 answers
The presence of pollutants can affect the overall rate of biodegradation. Organic contaminants, such as aromatic hydrocarbons, pesticides, and synthetic dyes, are not easily biodegradable and can accumulate in ecosystems, causing toxic symptoms in organisms, including humans. Microbial degradation processes play a central role in the biodegradation of environmental pollutants, and the types of enzymes and pathways utilized by microbes are key factors in this process. Xenobiotics, which include pollutants like carcinogens, drugs, and pesticides, are known for their persistence and can remain in the environment for prolonged periods. The slow kinetics of degradation and high toxicity of xenobiotics make their removal from contaminated environments challenging. However, alternative technologies, such as bio-electrochemical systems, show promise in improving the kinetics of biodegradation for rapid removal of xenobiotic contaminants from the environment.
What are the other names for sirtuin7?
5 answers
Sirtuin7 is also known as SIRT7.
What is the common name of sirtuin7?
5 answers
Sirtuin7 is commonly known as SIRT7.
Which is the mechanism of action of thioridazine on DR2?
5 answers
Thioridazine inhibits dopamine receptor 2 (DRD2) and this inhibition leads to the inhibition of self-renewal in certain triple-negative breast cancer cell lines. Thioridazine also induces cell death and inhibits proliferation in various cancer cells, including ovarian cancer cells. The anticancer effects of thioridazine in ovarian cancer are attributed to its ability to induce apoptosis and autophagy, increase reactive oxygen species levels, and cause DNA damage. In endometrial cancer cells, thioridazine enhances the effects of medroxyprogesterone on proliferative inhibition and apoptotic promotion, possibly through the PRB/DRD2-mediated PI3K/AKT signal pathway. Thioridazine also inhibits lipid peroxidation in model systems. Overall, the mechanism of action of thioridazine on DRD2 involves inhibiting self-renewal, inducing cell death, and affecting various signaling pathways in different cancer cell types.
How do cancer extracellular vesicles affect platelet function?
5 answers
Cancer extracellular vesicles, such as platelet-derived microvesicles (PMVs) and platelet extracellular vesicles (PEVs), have been shown to affect platelet function in various ways. PMVs can interact with cancer cells and nontransformed cells in the tumor microenvironment, transferring platelet-derived contents to the target cell and promoting disease progression. PEVs, which are formed during platelet activation or apoptosis, are highly heterogeneous and can transfer cargo molecules to recipient cells, potentially promoting cancer growth and metastasis. PEVs have been found to specifically bind to different breast cancer cells and elicit cell-specific functional responses, such as altering cell cycle progression and stimulating migration and invasion. Platelets and their derived extracellular vesicles can also contribute to cancer progression by changing the phenotype and functions of other cells, facilitating metastasis, and promoting angiogenesis. Overall, cancer extracellular vesicles have a complex and versatile impact on platelet function, influencing various aspects of cancer development and progression.
What gene related to breast cancer?
5 answers
Breast cancer is related to several genes, including BRCA1, BRCA2, TP53, CDH1, PTEN, STK1, PALB2, and CHEK2. These genes have been shown to have a strong genetic predisposition and are often inherited in an autosomal dominant manner. BRCA1 and BRCA2 are the most commonly encountered germline gene variants associated with breast cancer, followed by TP53, CDH1, PTEN, STK1, PALB2, and CHEK2. The prevalence of these genetic alterations may vary among different ethnic groups. Additionally, studies have identified ATM as another breast cancer susceptibility gene. Overall, these genes play a crucial role in breast cancer development and risk assessment.
What are the mechanisms by which mitochondria are affected by ?
5 answers
Mitochondria are affected by environmental toxins, such as bisphenol A (BPA), which disrupts mitochondrial functions through various molecular mechanisms. BPA impairs redox homeostasis, decreases antioxidant enzymes and mitochondrial complex activities, reduces ATP production, and causes mitochondrial dysfunction and apoptosis. Environmental toxins, including traffic-related air pollution, can also cause mitochondrial impairment, leading to damage to mitochondrial functions and overall bodily health. Additionally, changes in mitochondrial volume fraction can increase the localization of certain nuclear-encoded mRNAs to the surface of the mitochondria, modulating gene expression post-transcriptionally. Furthermore, mitochondria participate in immune regulation and exert immunoregulatory effects through mechanisms such as changes in mitochondrial dynamics, production of reactive oxygen species, and mitochondrial DNA damage. Disorders in mitochondrial fusion, division, and mobility can lead to defects in the functioning of the nervous system.
What are the effects of Tithonia diversifolia on cancer cells?
5 answers
Tithonia diversifolia has shown cytotoxic and antiproliferative effects on various cancer cell lines, including colon, glioblastoma, hepatoma, kidney, breast, lung, melanoma, leukemia, ovary, prostate, and stomach cancers. Compounds isolated from T. diversifolia, such as tagitinin A, 1β-hydroxytirotundin 3-O-methyl ether, and tagitinin C, have demonstrated cytotoxic activity against acute myeloid leukemia cells and five human cancer cell lines, including A549, T24, Huh-7, 8505, and SNU-1. Additionally, T. diversifolia leaf ethanolic extract has exhibited strong cytotoxic and antiproliferative activity on HeLa cervical cancer cells. Furthermore, T. diversifolia has been found to have potential as an anticancer agent, particularly for breast cancer, as it showed cytotoxic effects on T47D, MCF-7, and EVSA-T breast cancer cells. Overall, these findings suggest that Tithonia diversifolia has promising effects on various cancer cells and may have potential as a natural anticancer therapy.
Does sulfur treatment of garlic affect allicin concentration?
5 answers
Sulfur treatment of garlic has been shown to affect allicin concentration. In a field trial, the application of 75 kg S ha−1 resulted in the highest allicin content in the 'Glenlarge' cultivar. Similarly, sulfur fertilization at concentrations of 0.01 and 1.50 mmol L−1 increased allicin concentration in A. roseum bulbs. Allicin has been found to reduce cell viability and cell proliferation in various mammalian cell lines. Furthermore, allicin exposure has been shown to cause S-thioallylation of proteins in human Jurkat T-cells, affecting essential cellular functions. However, it is important to note that there is an inverse relationship between garlic bulb yield and allicin concentration, although some varieties have been found to have both high yield and allicin concentration. Overall, sulfur treatment can positively influence allicin concentration in garlic, but the specific effects may vary depending on the cultivar and experimental conditions.