How much time does cell cycle of yeast takes?
Answers from top 10 papers
15 Jun 2009-Genes & Development
|Here we show that in budding yeast, the ability of cells to grow changes during the cell cycle.|
15 Mar 1978-Experimental Cell Research
|2 h. Thus the cell cycle of yeast can be divided into an expandable phase from cell division to the initiation of DNA synthesis, the length of which is dependent on growth rate and a constant phase from the initiation of DNA synthesis to cell division which takes a constant time independent of generation time.|
|These findings underscore and extend earlier conclusions that most of the G1 interval of the yeast cell cycle is simply a period of ongoing growth.|
01 Mar 1978-Experimental Cell Research
|These observations are predicted if a yeast cell requires a critical size before a particular cell cycle event can be completed and that after completion of this event cell division occurs following a period of time independent of growth rate.|
|We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.|
01 Feb 1992-Journal of Biotechnology
|The characteristics and the time course of the yeast cell cycle were found to be strongly dependent on the physiological environment.|
|These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell.|
01 Nov 2018-Genetics
|These data reveal fundamental scaling relationships between the duration of eukaryotic cell cycle phases and rates of cell proliferation, point to the necessary role of Cln3p in these relationships in yeast, and provide a mechanistic basis linking Cln3p levels to proliferation rates and the scaling of G1 with doubling time.|
|The application to yeast cell-cycle data establishes a natural time order of genes that is in line with cell-cycle phases.|
23 Sep 1999-Nature
|Here we present evidence for an independent cell-cycle oscillator in the budding yeast Saccharomyces cerevisiae.|
Candida albicans growth curve4 answersCandida albicans growth curve was studied using growth curve methodology, which was found to be more accurate than the microbial sensitivity test for evaluating the occurrence of the paradoxical effect. The growth curve of Candida albicans was also investigated in the presence of cadmium, and it was found that the organism was resistant to this heavy metal at a concentration of 50 µg/ml. The growth rate of C. albicans was influenced by environmental conditions such as pH, temperature, and culture medium, with the fastest growth rate observed at 37°C in modified Sabouraud glucose broth medium with pH 7.4. Differences in growth kinetic parameters were found between different Candida species, with C. glabrata being the fastest growing species and C. parapsilosis showing the longest lag phase. Planktonic growth and biofilm formation of Candida haemulonii species complex were also studied, with all clinical isolates forming biofilm on polystyrene in a time-dependent manner.
How to DNA sequence a yeast?3 answersDNA sequencing of yeast can be done using various methods. One protocol involves preparing yeast DNA by digesting the cell wall and lysing the resulting spheroplasts with SDS. This method yields several micrograms of yeast DNA that can be cleaved by restriction enzymes and used as a template in polymerase chain reaction (PCR). Another method involves preparing a DNA sequencing library from yeast genomic DNA for use with the Illumina sequencing platform. This method utilizes specific reagents purchased largely from New England BioLabs, reducing the cost of library preparation. The shearing and size selection steps can be modified for different insert sizes. Additionally, there are simple protocols for preparing templates for direct sequencing of yeast mitochondrial DNA (mtDNA) using automatic DNA analyzers. These protocols yield sufficient quantity and quality of template DNA and can be used for re-sequencing of mtDNA for comparative analyses of yeast strains.
How yeast makes ATP?3 answersYeast produces ATP through the activity of the mitochondrial ATP synthase, a large multisubunit complex responsible for ATP synthesis. The enzyme complex consists of a water-soluble F1 sector and a membrane-embedded F0 sector. The F0 sector contains a ring of subunits that rotates in response to proton translocation, while the F1 sector contains the catalytic sites for ATP synthesis. The rotation of the F0 subunit ring drives conformational changes in the F1 sector, leading to ATP synthesis. The yeast ATP synthase is encoded by both the nuclear and mitochondrial genomes, with most subunits encoded by the nuclear genome and imported into mitochondria. Loss of respiration in yeast affects energy-intensive processes such as the maintenance of the plasma membrane proton gradient and nutrient import. The assembly of the yeast ATP synthase involves two separate but coordinated pathways that converge at the end stage.
How does the histone coverage of DNA change in yeast replicative ageing?5 answersDuring yeast replicative ageing, there is a decrease in histone coverage of DNA. Nucleosome occupancy across the genome decreases by 50% during replicative ageing, leading to a loss of histones. This loss of histones is accompanied by a transcriptional induction of all yeast genes. Specifically, genes that are normally repressed by promoter nucleosomes are most induced, indicating a preferential loss of nucleosomes from their promoters. Additionally, there is an increase in ubiquitylation of histone H2B at telomeric heterochromatin regions in replicatively aged cells. These changes in histone coverage and modifications are associated with cellular ageing and have implications for lifespan regulation.
Can you summarize the Cellular quiescence in budding yeast?3 answersCellular quiescence in budding yeast is a reversible state where cells temporarily exit the cell cycle. Quiescence is induced in response to nutrient starvation signals and involves large-scale remodeling of gene expression, organelles, and metabolism. The process of quiescence consists of three phases: initiation, maintenance, and exit. Quiescent cells are viable and play important roles in diseases such as cancer. Understanding cell quiescence is crucial for developing strategies to target quiescent cells. Single-cell approaches are necessary to address the heterogeneity among quiescent cells. Research on cellular quiescence in budding yeast has provided valuable insights into the molecular bases and transitions involved in quiescence.
How long does it take DNA replication to occur?5 answers
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5 answersSirtuin7 is also known as SIRT7.What is the common name of sirtuin7?
5 answersSirtuin7 is commonly known as SIRT7.Which is the mechanism of action of thioridazine on DR2?
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5 answersMitochondria are affected by environmental toxins, such as bisphenol A (BPA), which disrupts mitochondrial functions through various molecular mechanisms. BPA impairs redox homeostasis, decreases antioxidant enzymes and mitochondrial complex activities, reduces ATP production, and causes mitochondrial dysfunction and apoptosis. Environmental toxins, including traffic-related air pollution, can also cause mitochondrial impairment, leading to damage to mitochondrial functions and overall bodily health. Additionally, changes in mitochondrial volume fraction can increase the localization of certain nuclear-encoded mRNAs to the surface of the mitochondria, modulating gene expression post-transcriptionally. Furthermore, mitochondria participate in immune regulation and exert immunoregulatory effects through mechanisms such as changes in mitochondrial dynamics, production of reactive oxygen species, and mitochondrial DNA damage. Disorders in mitochondrial fusion, division, and mobility can lead to defects in the functioning of the nervous system.What are the effects of Tithonia diversifolia on cancer cells?
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5 answersSulfur treatment of garlic has been shown to affect allicin concentration. In a field trial, the application of 75 kg S ha−1 resulted in the highest allicin content in the 'Glenlarge' cultivar. Similarly, sulfur fertilization at concentrations of 0.01 and 1.50 mmol L−1 increased allicin concentration in A. roseum bulbs. Allicin has been found to reduce cell viability and cell proliferation in various mammalian cell lines. Furthermore, allicin exposure has been shown to cause S-thioallylation of proteins in human Jurkat T-cells, affecting essential cellular functions. However, it is important to note that there is an inverse relationship between garlic bulb yield and allicin concentration, although some varieties have been found to have both high yield and allicin concentration. Overall, sulfur treatment can positively influence allicin concentration in garlic, but the specific effects may vary depending on the cultivar and experimental conditions.