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SummaryWhen multiple types of cells from normal and diseased human skin are required, techniques to isolate cells from small skin biopsies would facilitate experimental studies.
This technique allows these skin equivalents to be easily mounted on Franz diffusion cells.
Given its easy accessibility, stem cells in skin will not only provide an experimental model for skin biology, but also may provide an experimental model for studying the epithelial-mesenchymal interactions of several other organs outside of skin.
The results demonstrate the capacity of ES cells to reconstitute in vitro a fully differentiated skin.
Cultured human skin cells are a potentially useful model for skin irritancy testing.
These studies underline the complex interactions between skin cells.

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