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How to interpret RNA Tapestation results? 

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Journal ArticleDOI
Steve L. Reichow, Gabriel Varani 
29 Jun 2006-Nature
11 Citations
These findings reveal how RNA folds to form a precise pocket for its target and how the antibiotic pyrithiamine acts by tricking the riboswitch.
The results should help to improve predictions of RNA secondary structure.
Results indicate that RNA "tracks" extend from an internal genome into the nuclear periphery, and that RNA transport may be coupled to transcription.
These results define the structure of RNA for full RIG-I activation and explain how RIG-I detects negative-strand RNA viruses that lack long double-stranded RNA but do contain blunt short double-stranded 5' triphosphate RNA in the panhandle region of their single-stranded genome.
Open accessJournal ArticleDOI
Xiaojun Xu, Shi-Jie Chen 
22 Sep 2016-PLOS ONE
14 Citations
The results allow users to gain extensive physical insights into RNA-RNA interactions and their roles in RNA function.
Significance: These findings help to explain how RNase R efficiently degrades structured RNA.
RESULTS High-throughput sequencing of small RNA populations allows the locations of the double-stranded RNA precursors to be inferred.
This study provides an exceptionally quantitative view of how an RNA molecule folds.
We interpret these results as indicating that the tail is not required for RNA assembly but is required for the template to function in RNA synthesis.
Importantly, we demonstrate how the relative amount of 4sU-labeled nascent RNA influences accurate quantification.

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