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How to study angiogenesis in murine vein graft? 


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To study angiogenesis in murine vein grafts, researchers have utilized various methods. One approach involved developing a two-photon intravital microscopy (2P-IVM) technique to assess the leakiness of plaque microvessels in atherosclerosis-prone ApoE3*Leiden vein grafts. Another study focused on inhibiting glycolysis in endothelial cells to prevent intraplaque angiogenesis and unstable plaque formation in vein grafts, showcasing a reduction in lesion size, neovessels, and hemorrhages. Additionally, a small molecule bFGF-inhibitor, K5, was tested to decrease intraplaque angiogenesis and hemorrhage in accelerated atherosclerotic vein graft lesions, demonstrating increased plaque stability. Furthermore, extracorporeal shock-wave treatment was shown to enhance revascularization of full-thickness skin isografts in mice, promoting early pro-angiogenic effects and suppressing inflammation. These studies collectively highlight diverse methodologies to investigate and modulate angiogenesis in murine vein graft models.

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Not addressed in the paper.
To study angiogenesis in murine vein grafts, assess revascularization, vessel number, density, and gene expression using techniques like microscopy, immunohistochemistry, and gene arrays as demonstrated in the paper.
Angiogenesis in murine vein grafts can be studied by assessing intraplaque angiogenesis and macrophage infiltration, which can be modulated by bFGF blockade using K5 in ApoE3*Leiden mice.
Study angiogenesis in murine vein graft by deleting PFKFB3 gene in endothelial cells, inhibiting intraplaque angiogenesis, reducing lesion size, neovessels, and hemorrhages, thus slowing plaque progression.
Study angiogenesis in murine vein grafts using two-photon intravital microscopy (2P-IVM) to assess microvessel permeability by quantifying fluorescent-dextrans extravasation in real-time, enabling evaluation of plaque microvessel leakiness.

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