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Is Cody Hackman related to Gene Hackman? 

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Although CodY is a strong repressor of the agr locus, CodY bound only weakly to the agrBDCA-RNAIII promoter region, suggesting that direct regulation by CodY is unlikely to be the principal mechanism by which CodY regulates agr and RNAIII expression.
Under these conditions, CodY loses the ability to repress genes (e. g., metabolic genes) and functions as a direct activator of the master virulence regulator gene, prfA.
Of the 389 DNA binding sites that were copurified with CodY, 132 sites were in or near the regulatory regions governing the expression of 197 CodY-controlled genes, indicating that CodY controls many other genes indirectly.
CodY is likely to play an important role in regulating overall cellular physiology as well.
GTP enhanced the binding affinity of CodY to the botA promoter, suggesting that CodY-dependent neurotoxin regulation is associated with nutritional status.
This observation raised the possibility that CodY possesses multiple functions that allow it to coordinate gene expression across a wide spectrum of metabolic growth conditions, and thus better adapt bacteria to the mammalian niche.
CodY acts by directly, and indirectly (via Agr), repressing toxin production during times of plentiful nutrition.
We demonstrate here that CodY is indeed active (i. e., binds DNA) under both conditions, serving as a repressor and activator of different genes.
In vitro, CodY represses the transcription of virulence genes, but it is not known if CodY also represses virulence in vivo.
Thus, in B. subtilis, CodY can now be seen to regulate the entire protein utilization pathway.

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