Is mutant protein any good?
Answers from top 10 papers
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| Papers (10) | Insight |
|---|---|
15 Mar 2016 | Ample literature evidences confirm the suitability of this protein as a good target. |
| This mutant protein is more efficiently GPI-modified than any mutant AChE tested thus far. | |
| The mutant protein appears to have a dominant negative effect, which could be due to it dimerising with the wild-type protein and inhibiting its activity, thus further reducing the levels of functional protein. | |
| Increasing gene expression restored mutant fitness to varying degrees, demonstrating that the fitness disadvantage of synonymous mutants arose from a lack of beneficial protein rather than costs of protein production. | |
10 Citations | The high level of success shows that relatively "simple" computational methods can in some cases predict the behavior of mutant proteins with good reliability. |
19 Citations | In some cases, therefore, it is possible that the mutant protein exerts a dominant negative effect. |
| In the case of interference, increased production of the wild-type protein may be therapeutic, but in the case of toxicity, suppression of the mutant protein may also be needed. | |
32 Citations | Cells of the mutant are viable, showing that the protein is dispensable for growth, but grow slower and have thickened cell walls. |
21 Citations | Although this scheme requires no knowledge of the structure of a mutant protein, it is useful in predicting the activities of mutants. |
| The swapped mutant was destabilized by 3 kcal/mol per gene V protein dimer relative to the wild-type protein, demonstrating that factors other than hydrophobicity must make substantial contributions to the effects of mutations on the stability of the protein. |
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