What are the different aspects of measurement of amino acids in plasma using HPLC?
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| HPLC methods using pre-column derivatization with o-phthaldialdehyde were suitable for the accurate determination of many primary amino acids in plasma because of their high sensitivity, simplicity, speed and reliability. | |
42 Citations | Thus, the HPCE‐based procedure presented here for the measurement of sulfur‐containing amino acids in plasma is a simple, fast, accurate, and very sensitive method, suitable for routine determinations in clinical studies. |
| In the present work, most of the plasma amino acids were assayed with high sensitivity, accuracy and good reproducibility in a relatively short time and on very small amounts of sample. | |
| The precision, stability, and sensitivity of the method render it ideal for the quantitation of plasma amino acids. | |
| The results of the specificity, linearity, accuracy, repeatability, intermediate precision, reproducibility, limits of detections, lower limits of quantification, carry over, and sample solution stability were sufficient to allow for the measurement of amino acids in human plasma samples. | |
Open access•Journal Article 17 Citations | A new automated HPLC system is introduced for the quantitation of both primary and secondary amino acids; the sensitivity and speed of this system is especially useful for screening large numbers of physiological fluids. |
| These results demonstrated that the proposed method is sensitive, accurate, and efficient for direct quantitative analysis of aromatic amino acids in human plasma. | |
255 Citations | The resulting improved accuracy in measurement of plasma amino acids may be essential for meaningful studies of some metabolic or nutritional disorders where differences from normal are subtle. |
163 Citations | Muscle, liver, and kidney free amino acid concentrations, as determined by HPLC, are in the expected physiological range and compare favorably with those obtained by conventional amino acid analyzer. |
54 Citations | The present study, apart from establishing the normal profile of amino acids in plasma of Indian sub population, also presents HPLC profile for some of the rare amino acidopathies. |
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