What does it mean to clone a cell phone?
Answers from top 8 papers
More filters
| Papers (8) | Insight |
|---|---|
125 Citations | These results suggest that the clone‐derived cells have fully acquired an ES cell character. |
| We show that while cell phone reuse has a healthy profit margin, handset recycling is currently a by-product of reuse. | |
| The results of a large industrial case study indicate that it can significantly increase clone detection accuracy. | |
| Thus, although this cytotoxic T-cell clone does have useful in vivo activity, its function may be partially limited by a generalized defect in migration. | |
45 Citations | Previously, a CD8+ T cell clone, I. S. |
| In some instances, it is even advantageous not to clone the products, as described in other chapters. | |
| We believe it is necessary to have a better understanding of how developers approach and remove duplication to improve clone detectors as well as clone refactoring tools. | |
133 Citations | This result provides evidence for the pluripotentiality of the clone-forming cell. |
Related Questions
What are the type of cloning techniques?4 answersThere are several types of cloning techniques available. One approach is ligation-independent cloning, which includes methods such as polymerase incomplete primer extension (PIPE) cloning, sequence and ligation-independent cloning (SLIC), and overlap extension cloning (OEC). These techniques rely on generating complementary overhangs by DNA polymerase, eliminating the need for specific restriction sites or ligation. Another type of cloning technique involves using zygotes as recipients for cell nuclear transfer. Microsurgical methods are used to remove pronuclei from zygotes, breaking them and leaving behind nuclear components in the cytoplasm, making the enucleated zygotes suitable for cloning. Additionally, there are cloning techniques specifically related to data storage systems, such as creating writable clones of virtual volumes (vvols). These techniques involve creating a base snapshot, creating a new vvol as a clone, and instantiating the clone by loading a file system onto it.
What is cloning?5 answersCloning is the process of creating genetically identical individuals. It has different meanings depending on the context. In the field of science, cloning has been used to refer to natural reproduction in bacteria, horticultural practices, and various molecular technologies in vertebrates. In the context of database systems, cloning refers to creating a point-in-time copy of a database for testing or data analysis purposes. Gene cloning involves generating identical copies of a cell or organism by cutting DNA into fragments and cloning them onto a vector, which can be used for various applications in medicine, agriculture, and research. Animal cloning is a reproductive technology that allows the creation of identical genetic twins from existing animals, with applications in breeding and genetic trait replication. Cloning also has the potential for generating copies of elite animals for livestock breeding and establishing herds of transgenic animals for pharmaceutical production.
What is cloning?5 answersCloning is the process of creating genetically identical individuals. It has different meanings depending on the context. In the field of science, cloning has been used to refer to natural reproduction in bacteria, horticultural practices, and various molecular technologies in vertebrates. In the context of database systems, cloning refers to creating a point-in-time copy of a database for testing or data analysis purposes. Gene cloning involves generating identical copies of a cell or organism by cutting DNA into fragments and cloning them onto a vector, which can be used for various applications in medicine, agriculture, and research. Animal cloning is a reproductive technology that allows the creation of genetically identical individuals for breeding purposes, but it faces challenges in terms of low survival rates. Cloning has potential applications in livestock breeding and the production of pharmaceuticals, but ethical considerations have limited its use in human cloning.
The steps to handmade cloning?4 answersHandmade cloning (HMC) is a method of somatic cell nuclear transfer (SCNT) that eliminates the need for micromanipulators. The steps involved in HMC include: (1) preparing the donor cells, such as fetal fibroblasts, for constructing the cloned embryos, (2) preparing the recipient egg cytoplasm for constructing the cloned embryos, (3) performing handmade enucleation of the oocytes, which involves removing the somatic nucleus and transferring it into an enucleated oocyte, (4) reconstructing the buffalo transgenic cloned embryos, (5) activating the reconstructed embryos chemically, and (6) culturing the cloned embryos to allow their development. HMC has been shown to be a simple and efficient method for large-scale embryo production, with high blastocyst rates and successful births in various species, including water buffalo and pigs.
What is molecualr cloning?4 answersMolecular cloning is a technique used to isolate and create multiple copies of a specific DNA fragment in a host organism. It is a fundamental tool in molecular and cellular biology research, allowing the study of individual DNA sequences and the generation of complex combinations of DNA fragments for various applications. The process involves the isolation of a DNA fragment of interest, its insertion into a vector, and the replication of the recombinant DNA in a host organism. Molecular cloning has been widely used in labs worldwide for over 30 years and has been continuously updated to include the most valuable and widely used nucleic-acid based methods. It is essential for various applications, including gene transfer, expression analysis, and the preparation and manipulation of DNA, RNA, and proteins. The techniques used in molecular cloning are covered in detail in laboratory manuals, providing students with hands-on experience in subcloning genes and purifying recombinant proteins.
What is the concept of recombinant DNA technology in cloning?4 answersRecombinant DNA technology in cloning involves the creation of hybrid DNA by inserting a foreign sequence into the DNA of another species. This is achieved by extracting the gene of interest and incorporating it into a self-replicating vector, such as a plasmid. The vector's DNA is cleaved using a restriction endonuclease and then sealed back using DNA ligase. The vector is then inserted into a host organism, such as E. coli. The plasmid will multiply within the host, and the host's translation machinery will synthesize proteins from the inserted gene. This technology has been used to synthesize hormones, vaccines, drugs, and genetically modified food, as well as in gene therapy. Biosimilars, which are similar products manufactured using recombinant DNA technology, have also been developed. Recombinant DNA technology allows for the manipulation and study of genes involved in biological pathways. It involves recombining genes from different sources and expressing them in a host organism, typically E. coli. This technology has advanced rapidly, with new techniques being discovered and applied in gene cloning, restriction endonucleases, DNA sequencing, and protein expression. Cloning procedures using recombinant DNA technology involve amplifying, cutting, and ligating DNA in vitro. This allows for the creation of recombinant clones, which can be used to study gene function, visualize proteins, and facilitate protein purification. DNA cloning in episomes or plasmids of bacteria and simple eukaryotes is a cost-effective method for amplifying DNA with high precision. It involves the generation of recombinant plasmids by joining plasmid vector DNA and insert DNA fragments using DNA ligase. These recombinant plasmids can be introduced into microbial organisms through transformation or transduction, and various selection methods can be used to identify the desired clones.