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What stain is used for Mycobacterium tuberculosis? 

Tuberculin
Mycobacterium tuberculosis
Mycobacterium tuberculosis complex
Tuberculosis
Nucleic acid amplification technique

Answers from top 7 papers

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Papers (7)Insight
Journal Article•DOI
Antibodies to Mycobacteria in Human Tuberculosis. I. Development of Antibodies Before and After Antimicrobial Therapy
Mark H. Kaplan, Mark H. Kaplan, Merrill W. Chase, Merrill W. Chase 
01 Dec 1980-The Journal of Infectious Diseases
56 Citations
These microimmunodiffusion tests appear to be specific for Mycobacterium tuberculosis.
Open access•Journal Article•DOI
Comparison of an Internally Controlled, Large-Volume LightCycler Assay for Detection of Mycobacterium tuberculosis in Clinical Samples with the COBAS AMPLICOR Assay
Siegfried Burggraf, Udo Reischl, Naeem Malik, Markus Bollwein, Ludmila Naumann, Bernhard Olgemöller 
01 Apr 2005-Journal of Clinical Microbiology
30 Citations
We present a sensitive and specific assay for reliable and flexible detection of members of the Mycobacterium tuberculosis complex (MTBC) in clinical samples.
Open access•Journal Article•DOI
Exclusion of active Mycobacterium tuberculosis complex infection with the T-SPOT.TB assay.
R. M. L. van Leeuwen, A. W. J. Bossink, S. F. T. Thijsen 
01 Mar 2007-European Respiratory Journal
22 Citations
This test proved to be a useful tool to help rule out the diagnosis of active Mycobacterium tuberculosis infection and could therefore prevent unnecessary or inappropriate therapy.
Journal Article•DOI
Gas chromatography–mass spectrometry methods for the analysis of mycocerosic acids present in Mycobacterium tuberculosis
Srinivas Alugupalli, Monica K Sikka, Lennart Larsson, David C. White 
01 Jan 1998-Journal of Microbiological Methods
12 Citations
The described methods can be used to demonstrate and quantify certain species of Mycobacterium, including M. tuberculosis, in clinical or environmental samples.
Open access•Journal Article•DOI
Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies
Fabio Cimaglia, Alessandro Aliverti, Maurizio Chiesa, Palmiro Poltronieri, Enrico De Lorenzis, Angelo Santino, Leonardo Antonio Sechi 
04 Jan 2012-Nanotechnology Development
15 Citations
The reported technology could be useful in the diagnostic investigation of Mycobacterium tuberculosis and other human pathogens in clinical specimens.
Open access•Journal Article•DOI
Integrating the Xpert MTB/RIF Assay into a Diagnostic Workflow for Rapid Detection of Mycobacterium tuberculosis in a Low-Prevalence Area
Vanessa Deggim, Akos Somoskovi, Antje Voit, Erik C. Böttger, Guido V. Bloemberg 
01 Jul 2013-Journal of Clinical Microbiology
27 Citations
The results showed that replacing smear microscopy with the Xpert MTB/RIF assay facilitates laboratory handling and improves the sensitivity and specificity of Mycobacterium tuberculosis detection.
Journal Article•DOI
Non-tuberculous mycobacteria infection: 75 cases.
Aylin Babalik, Tülin Kuyucu, Emine Nilgün Ordu, Dilek Ernam, Mualla Partal, Kaya Koksalan 
01 Jan 2012-Tüberküloz ve toraks
13 Citations
In order to carry out the right treatment and epidemiologic evaluation, it is important to identify non-tuberculosis mycobacterium by culture methods.

Related Questions

Which biomarkers are most useful for the diagnosis of mycobacterium tuberculosis?5 answersThe biomarkers AIM2 and FCGR1A have shown promise for the diagnosis of Mycobacterium tuberculosis (MTB) infection. An optical biosensor chip using localized surface plasmon resonance (LSPR) has been developed to detect biomolecular interactions between mycolic acid TB antigen and anti-Mycobacterium tuberculosis antibody. Another study has proposed an electrochemical biosensor based on a ternary nanocomposite of reduced graphene oxide, polydopamine, and gold nanoparticles (rGO-PDA-AuNP) for MTB detection. Additionally, a combined quantitative tuberculosis biomarker model using colony forming unit (CFU) and time-to-positivity (TTP) biomarkers has been developed to assess drug efficacy in early bactericidal activity studies. Furthermore, the expression of certain genes, such as icl1, has shown potential for monitoring differentially detectable M. tuberculosis populations in patient sputum.
What new diagnostic tools can be developed to improve the detection of drug-resistant strains of Mycobacterium tuberculosis?4 answersNew diagnostic tools can be developed to improve the detection of drug-resistant strains of Mycobacterium tuberculosis. One approach is the use of multiplexed, targeted next generation sequencing (tNGS) assays that can predict drug resistance (DR) directly from clinical respiratory specimens. Another potential tool is metabolomics, which can identify metabolite markers to detect pre-extensively drug-resistant (pre-XDR) and extensively drug-resistant tuberculosis (XDR-TB). Additionally, reference graphs can be used to represent genetic variation within a bacterial population, allowing for the prediction of drug resistance in bacteria like M. tuberculosis. These tools offer the potential for faster and more accurate detection of drug-resistant strains, enabling timely and appropriate treatment for patients with drug-resistant tuberculosis.
What are stain types and characteristics?5 answersStains used in cytological and histological analysis can be categorized into different types based on their characteristics. Some stains are nonspecific and can detect a spectrum of organisms, while others are specific for a particular organism. Nonspecific stains like the Papanicolaou stain and silver impregnation methods can detect a wide range of organisms, while acid-fast techniques can detect only a limited group of organisms. Special stains are essential in routine laboratory works and can be used to demonstrate various cellular products for diagnosis, identify microbial organisms, and estimate DNA and RNA content of cells. Stains can also be used to contrast the nuclear portion of cells from the cytoplasmic portion, and systems and methods for illuminating cytological samples can be employed. Additionally, stains can be used in wood finishing, with water stains, oil stains, and spirit stains being the three main types used for staining wood.
How does methylene blue stain bacteria?5 answersMethylene blue stains bacteria by using a primary stain that does not require heating and a decolorizing counter-stain that does not require a separate acid or alcohol decolorization step. The primary stain includes basic fuchsin, ethyl alcohol, isopropyl alcohol, phenol, and water, while the counter-stain includes methylene blue, ethyl alcohol, potassium hydroxide, glycerol, glacial acetic acid, polyvinylpyrrolidone, and water. Another method of staining bacteria involves a mixture of methylene blue and eosin Y, which gives a deep staining of bacteria at pH 3 and above. This mixture can be successfully used at a pH-value as low as 0.8 and is sensitive enough to detect differences in the isoelectric points of bacteria. Methylene blue has also been found to impair the repair activity of the polymerase 1 enzyme in Escherichia coli cells, sensitizing these bacteria to the lethal effects of ionizing radiation.
Which bacteria is used for tuberculosis?11 answers
Which staining technique is used in the diagnosis of tuberculosis and leprosy?3 answers

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