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What stain is used to identify Mycobacterium tuberculosis? 

Mycobacterium tuberculosis
Mycobacterium tuberculosis complex
Tuberculosis diagnosis
Tuberculosis
Nucleic acid amplification technique

Answers from top 7 papers

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Papers (7)Insight
Journal Article•DOI
Gas chromatography–mass spectrometry methods for the analysis of mycocerosic acids present in Mycobacterium tuberculosis
Srinivas Alugupalli, Monica K Sikka, Lennart Larsson, David C. White 
01 Jan 1998-Journal of Microbiological Methods
12 Citations
The described methods can be used to demonstrate and quantify certain species of Mycobacterium, including M. tuberculosis, in clinical or environmental samples.
Journal Article•DOI
Multiplex polymerase chain reaction for rapid detection of atypical mycobacteria and Mycobacterium tuberculosis complex.
Klemen H, Bogiatzis A, Ghalibafian M, Popper Hh 
01 Dec 1998-Diagnostic Molecular Pathology
33 Citations
Therefore, this method is a quick, cost efficient, and reliable tool to identify mycobacteria other than the tuberculosis complex
Open access•Journal Article•DOI
Gamma Interferon Release Assays for Detection of Mycobacterium tuberculosis Infection
Madhukar Pai, Claudia M. Denkinger, Claudia M. Denkinger, Sandra V. Kik, Molebogeng X. Rangaka, Alice Zwerling, Olivia Oxlade, John Z. Metcalfe, Adithya Cattamanchi, David W. Dowdy, Keertan Dheda, Niaz Banaei 
01 Jan 2014-Clinical Microbiology Reviews
642 Citations
They represent indirect markers of Mycobacterium tuberculosis exposure and indicate a cellular immune response to M. tuberculosis.
Open access•Journal Article•DOI
Quantum dots nanoparticle-based lateral flow assay for rapid detection of Mycobacterium species using anti-FprA antibodies
Fabio Cimaglia, Alessandro Aliverti, Maurizio Chiesa, Palmiro Poltronieri, Enrico De Lorenzis, Angelo Santino, Leonardo Antonio Sechi 
04 Jan 2012-Nanotechnology Development
15 Citations
The reported technology could be useful in the diagnostic investigation of Mycobacterium tuberculosis and other human pathogens in clinical specimens.
Journal Article•DOI
Length of time to laboratory diagnosis of Mycobacterium tuberculosis infection: comparison of in-house methods with reference laboratory results.
Angharad P. Davies, L E Newport, O.J. Billington, Stephen H. Gillespie 
01 Nov 1999-Journal of Infection
16 Citations
Development of on-site laboratory facilities to identify Mycobacterium tuberculosis can reduce the time to its identification by almost two-thirds.
Open access•Journal Article•DOI
Comparison of an Internally Controlled, Large-Volume LightCycler Assay for Detection of Mycobacterium tuberculosis in Clinical Samples with the COBAS AMPLICOR Assay
Siegfried Burggraf, Udo Reischl, Naeem Malik, Markus Bollwein, Ludmila Naumann, Bernhard Olgemöller 
01 Apr 2005-Journal of Clinical Microbiology
30 Citations
We present a sensitive and specific assay for reliable and flexible detection of members of the Mycobacterium tuberculosis complex (MTBC) in clinical samples.
Journal Article•DOI
Antibodies to Mycobacteria in Human Tuberculosis. I. Development of Antibodies Before and After Antimicrobial Therapy
Mark H. Kaplan, Mark H. Kaplan, Merrill W. Chase, Merrill W. Chase 
01 Dec 1980-The Journal of Infectious Diseases
56 Citations
These microimmunodiffusion tests appear to be specific for Mycobacterium tuberculosis.

Related Questions

How to diagnosis tuberculosis?5 answersTuberculosis can be diagnosed using various methods including clinical examination, radiography, sputum smear microscopy, culture method, serological method, tuberculin skin test, and molecular methods such as GeneXpert, loop-mediated isothermal amplification (LAMP), line probe assay (LPA), and whole genome sequencing (WGS). Non-invasive methods like lateral flow urine lipoarabinomannan assay (LF-LAM) and eNose assays are also available for tuberculosis diagnosis. Additionally, there are innovative diagnostic approaches such as an apparatus for simply diagnosing tuberculosis using a blood collection device. Another method involves pretreatment of the sample with an acidic material to increase the reactivity of tuberculous antigens and detection antibodies, enabling a point-of-care test for tuberculosis. These advancements aim to provide rapid, cost-effective, and point-of-care diagnostic methods for tuberculosis. However, further studies are needed to validate the accuracy and effectiveness of these diagnostic methods.
Which biomarkers are most useful for the diagnosis of mycobacterium tuberculosis?5 answersThe biomarkers AIM2 and FCGR1A have shown promise for the diagnosis of Mycobacterium tuberculosis (MTB) infection. An optical biosensor chip using localized surface plasmon resonance (LSPR) has been developed to detect biomolecular interactions between mycolic acid TB antigen and anti-Mycobacterium tuberculosis antibody. Another study has proposed an electrochemical biosensor based on a ternary nanocomposite of reduced graphene oxide, polydopamine, and gold nanoparticles (rGO-PDA-AuNP) for MTB detection. Additionally, a combined quantitative tuberculosis biomarker model using colony forming unit (CFU) and time-to-positivity (TTP) biomarkers has been developed to assess drug efficacy in early bactericidal activity studies. Furthermore, the expression of certain genes, such as icl1, has shown potential for monitoring differentially detectable M. tuberculosis populations in patient sputum.
How can biomarkers be used to improve the diagnosis of mycobacterium tuberculosis?5 answersBiomarkers can be used to improve the diagnosis of Mycobacterium tuberculosis (M. tuberculosis) by identifying novel diagnostic biomarkers for distinguishing different infection statuses of M. tuberculosis. These biomarkers can be identified through the analysis of differential genes (DEGs) from gene expression datasets. Protein-protein interaction networks can also be analyzed to understand the interactions between the proteins encoded by the DEGs. Additionally, real-time PCR and ELISA can be used to validate the expression levels of these biomarkers. By using these biomarkers, it is possible to distinguish between active tuberculosis (ATB), latent tuberculosis infection (LTBI), and healthy controls (HC). Furthermore, the use of biosensors, such as optical biosensor chips, can provide real-time analysis and high sensitivity for monitoring biomolecular interactions related to M. tuberculosis. Immunomics, which directly targets immune molecules, can also be utilized to identify immune biomarkers for diagnosing tuberculosis and predicting treatment outcomes. Finally, the transcriptional profiles of differentially detectable M. tuberculosis populations can be used to monitor these populations in patient sputum, providing insights into disease persistence and drug tolerance.
Can we differentiate between M tuberculosis and M bovis by AFB staining?6 answers
What type of stain is used to identify tuberculosis?4 answers
Which dye is used in fluorescence microscopy for tuberculosis?5 answers

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