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What type of stain is used to identify tuberculosis?

Polymerase chain reaction

Mycobacterium tuberculosis

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Journal Article•DOI Rapid detection of tuberculous and non-tuberculous mycobacteria by polymerase chain reaction amplification of a 162 bp DNA fragment from antigen 85. M. Fauville-Dufaux, B. Vanfleteren, L. De Wit, J. P. Vincke, J.P. Van Vooren, M. D. Yates, Elisabeth Schoutens Serruys - Show less +6 more 01 Sep 1992-European Journal of Clinical Microbiology & Infectious Diseases 36 Citations	The results suggest that this PCR assay could be used for early and specific diagnosis of tuberculosis.
Journal Article•DOI Identification of Mycobacterium tuberculosis DNA in Five Different Types of Cutaneous Lesions by the Polymerase Chain Reaction Neal S. Penneys, Craig L. Leonardi, Sandra Cook, Andrew Blauvelt, Steven A. Rosenberg, Louis D. Eells, Mark Konwiser, Charles M. Aaronson - Show less +7 more 01 Dec 1993-Archives of Dermatology 60 Citations	DNA diagnostic methods such as the polymerase chain reaction can be used to rapidly identify cutaneous lesions produced by M tuberculosis.
Open access•Journal Article•DOI Complications in the study of ancient tuberculosis: non-specificity of IS6110 PCRs Romy Müller, Charlotte A. Roberts, Terence A. Brown - Show less +2 more 06 Jan 2015 19 Citations	This test has been used frequently since the 1990s to identify tuberculosis in archaeological remains, but we show that it is not accurate.
Journal Article•DOI Molecular characterization of Mycobacterium tuberculosis isolates from Łódź, Poland: analysis by IS6110 restriction fragment length polymorphism and double-repetitive-element PCR. Agnieszka Dela, Anna Sajduda, Irena Pawłowska, JarosŁaw Dziadek - Show less +3 more 01 May 2006-Journal of Infection 11 Citations	Both methods used for the molecular characterization of M. tuberculosis clinical isolates showed similar discriminating ability.

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Related Questions

What are the potential benefits of using PCR for the diagnosis of tuberculosis?5 answersPCR has several potential benefits for the diagnosis of tuberculosis. It is a fast, safe, and reproducible method that allows for the early detection of Mycobacterium tuberculosis. PCR tests, such as real-time PCR and digital PCR, have shown high sensitivity in detecting TB infection, even in samples with low bacterial loads. These tests have also demonstrated improved specificity and accuracy compared to traditional diagnostic methods like smear microscopy and culture. Additionally, PCR assays can detect drug-resistance mutations in M. tuberculosis, providing critical information for determining the drug resistance status of a sample. The use of PCR for TB diagnosis has the potential to enhance case detection rates, leading to improved patient treatment and infection control.

What are stain types and characteristics?5 answersStains used in cytological and histological analysis can be categorized into different types based on their characteristics. Some stains are nonspecific and can detect a spectrum of organisms, while others are specific for a particular organism. Nonspecific stains like the Papanicolaou stain and silver impregnation methods can detect a wide range of organisms, while acid-fast techniques can detect only a limited group of organisms. Special stains are essential in routine laboratory works and can be used to demonstrate various cellular products for diagnosis, identify microbial organisms, and estimate DNA and RNA content of cells. Stains can also be used to contrast the nuclear portion of cells from the cytoplasmic portion, and systems and methods for illuminating cytological samples can be employed. Additionally, stains can be used in wood finishing, with water stains, oil stains, and spirit stains being the three main types used for staining wood.

What are the plant that give stain?5 answersPlant stains can be obtained from a variety of sources. Cotton and flax fibers, as well as plant cuticles, contain waxy components that can be stained using Oil Red. Essential oils from plants such as Cinnamomum cassia, Syzygium aromaticum, and Thymus mongolicus have been found to exhibit strong antifungal activities against wood mold and stain fungi. A plant stain remover has been developed using aloe, fructus gleditsiae, and cactus extracts, among other ingredients, which effectively removes stains from industrial materials. Another natural stain composition includes tea seed powder and vermiculite, providing a non-toxic and alkaline solution for stain removal. Additionally, a plant stain-removing skincare product has been developed, which utilizes plant extract active components to eliminate redundant melanin and reduce wrinkles.

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