Where is RNA primer made?
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| Papers (10) | Insight |
|---|---|
| The most probable origin of the primer(s) is viral RNase H-generated fragments of the viral RNA template. | |
74 Citations | Our results highlight the linker as directly involved in lifting the first boundary to processive RNA synthesis, the formation of the first dinucleotide primer. |
| Weiss and Nakamoto have presented preliminary evidence indicating that the relative position of ribonucleotides in enzymatically prepared RNA is determined by the primer used for the reaction.8 This information, along with the similar base composition found, suggests, but does not prove, that synthesis occurs with replication of the nucleotide sequences of the primer. | |
| While chemical synthesis and gel or chromatographic purification of RNA is costly and difficult for sequences longer than tens of nucleotides, a pipeline of primer assembly of DNA templates, in vitro transcription by T7 RNA polymerase and kit-based purification provides a cost-effective and fast alternative for preparing RNA molecules. | |
15 Citations | This result suggests that the substantial mass of RNA can be accommodated between the binding site for the primer (and nascent RNA) and the catalytic center of RNA polymerization. |
| We report here a server named Primerize that makes available an efficient algorithm for primer design developed and experimentally tested in our laboratory for RNA domains with lengths up to 300 nucleotides. | |
116 Citations | Covalent linkage between the RNA and the newly synthesized complementary DNA chain indicates that the RNA transcript serves as a primer. |
| Efforts to provide experimental support for the RNA world hypothesis have focused on producing such a polymerase, and in vitro evolution methods have led to the isolation of a polymerase ribozyme that catalyzes primer extension which is accurate and general, but slow. | |
152 Citations | The present study confirms that finding and demonstrates that, at least for the majority of 35S RNA molecules, the primer is bound at a site close to the 5'-terminus. |
| We show that RNA can serve as a primer in PCR. |
Related Questions
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