Which microscope is best for cell division in functional state?
10 answers found
The lens epithelium is, in several respects, very useful for studies on cell division.
Cellular Proliferation in the Lens
01 Aug 1965•Investigative Ophthalmology & Visual Science 17 citations
Thus an atomic force microscope can be used for high-resolution real-time studies of the dynamic subcellular mechanisms that drive cell behavior.
This imaging microscope is particularly useful for measurements of small-size samples that undergo rapid chemical or biochemical reactions, e. g., activities of a single biological cell.
Multispectral imaging microscope with millisecond time resolution.
03 Jan 2001•Analytical Chemistry 21 citations
We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy.
We present an easy-to-use combination of an atomic force microscope (AFM) and an epi-fluorescence microscope, which allows live cell imaging under physiological conditions.
A combined optical and atomic force microscope for live cell investigations
01 Jun 2006•Ultramicroscopy 64 citations
Our cell deposition microscope is capable of patterning different cell types onto and within standard cell research devices and providing on-stage incubation for long-term cell culturing.
After discussion of other methods it is concluded that the most useful general approach, at least for cultured cells, is to first permeabilize or break open the cells in a medium which preserves the structure under study in a functional state as, for example, the movement of chromosomes along the division spindle, or transport of proteins within the Golgi region.
This method will allow routine cell counting using a plain bright-field microscope without cell-line modification or cell staining.
Counting unstained, confluent cells by modified bright-field microscopy.
01 Jul 2013•BioTechniques 39 citations
The microscope will be easily portable by a rat or mouse and thus should enable functional imaging in freely behaving animals.
The unique configuration of this integrated microscope allows for the simultaneous acquisition of both anatomical (structural) and functional imaging information with particular emphasis for applications in the fields of tissue engineering and cell biology.
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