scispace - formally typeset
Search or ask a question

Which microscope is best for cell division in functional state? 

Microscope
Cell culture
Ultrastructure
Neurite
Microscopy

Answers from top 10 papers

More filters
Papers (10)Insight
Open access•Journal Article
Cellular Proliferation in the Lens
B. D. Srinivasan, C.V. Harding 
01 Aug 1965-Investigative Ophthalmology & Visual Science
17 Citations
The lens epithelium is, in several respects, very useful for studies on cell division.
Journal Article•DOI
Imaging real-time neurite outgrowth and cytoskeletal reorganization with an atomic force microscope.
Ratneshwar Lal, B. Drake, D. Blumberg, D. R. Saner, Paul K. Hansma, Stuart C. Feinstein 
01 Jul 1995-American Journal of Physiology-cell Physiology
62 Citations
Thus an atomic force microscope can be used for high-resolution real-time studies of the dynamic subcellular mechanisms that drive cell behavior.
Journal Article•DOI
Multispectral imaging microscope with millisecond time resolution.
Oleg Khait, Sergey V. Smirnov, Chieu D. Tran 
03 Jan 2001-Analytical Chemistry
21 Citations
This imaging microscope is particularly useful for measurements of small-size samples that undergo rapid chemical or biochemical reactions, e. g., activities of a single biological cell.
Open access•Journal Article•DOI
Same Serial Section Correlative Light and Energy-filtered Transmission Electron Microscopy
Ying Ren, Michael J. Kruhlak, David P. Bazett-Jones 
01 May 2003-Journal of Histochemistry and Cytochemistry
33 Citations
We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy.
Journal Article•DOI
A combined optical and atomic force microscope for live cell investigations
Josef Madl, Sebastian Rhode, Herbert Stangl, Hannes Stockinger, Peter Hinterdorfer, Gerhard J. Schütz, Gerald Kada 
01 Jun 2006-Ultramicroscopy
64 Citations
We present an easy-to-use combination of an atomic force microscope (AFM) and an epi-fluorescence microscope, which allows live cell imaging under physiological conditions.
Open access•Journal Article•DOI
Laser-guidance-based cell deposition microscope for heterotypic single-cell micropatterning
Zhen Ma, Russell K. Pirlo, Qin Wan, Julie X. Yun, Xiaocong Yuan, Peng Xiang, Thomas K. Borg, Bruce Z. Gao 
01 Jul 2011-Biofabrication
34 Citations
Our cell deposition microscope is capable of patterning different cell types onto and within standard cell research devices and providing on-stage incubation for long-term cell culturing.
Journal Article•DOI
Sample preparation for electron microscopy of internal cell structure
Geoffrey H. Haggis
01 Jul 1992-Microscopy Research and Technique
17 Citations
After discussion of other methods it is concluded that the most useful general approach, at least for cultured cells, is to first permeabilize or break open the cells in a medium which preserves the structure under study in a functional state as, for example, the movement of chromosomes along the division spindle, or transport of proteins within the Golgi region.
Open access•Journal Article•DOI
Counting unstained, confluent cells by modified bright-field microscopy.
L. Louis Drey, Michael C. Graber, Jan Bieschke 
01 Jul 2013-BioTechniques
40 Citations
This method will allow routine cell counting using a plain bright-field microscope without cell-line modification or cell staining.
Open access•Journal Article•DOI
Ultra-compact fiber-optic two-photon microscope for functional fluorescence imaging in vivo
Christoph J. Engelbrecht, Richard S. Johnston, Eric J. Seibel, Fritjof Helmchen 
14 Apr 2008-Optics Express
282 Citations
The microscope will be easily portable by a rat or mouse and thus should enable functional imaging in freely behaving animals.
Open access•Journal Article•DOI
Integrated structural and functional optical imaging combining spectral-domain optical coherence and multiphoton microscopy
Claudio Vinegoni, Tyler S. Ralston, Wei Tan, Wei Luo, Daniel L. Marks, Stephen A. Boppart 
31 Jan 2006-Applied Physics Letters
94 Citations
The unique configuration of this integrated microscope allows for the simultaneous acquisition of both anatomical (structural) and functional imaging information with particular emphasis for applications in the fields of tissue engineering and cell biology.

Related Questions

What is the role of cell division in the growth and development of multicellular organisms?5 answersCell division plays a crucial role in the growth and development of multicellular organisms by determining cell arrangement, size, and shape. It influences tissue morphology by reducing mechanical stress and reinforcing the cell wall network, potentially aligning with maximal tensile stress directions. Understanding the molecular mechanisms governing cell division is vital to prevent aberrant divisions leading to tumor formation. In animal cells, defects in cell growth and division pathways can result in abnormal proliferation and cancer development. The pattern of cell division is essential for proper development, with animal cytokinesis mediated by a contractile ring and plant cytokinesis involving the assembly of a cell plate from Golgi-derived vesicles. Overall, cell division is intricately linked to mechanical stress, tissue morphology, and proper development in multicellular organisms.
What happens during cell division?5 answersDuring cell division, eukaryotic cells undergo a coordinated remodelling of their cytoskeleton and membranes. This includes the segregation of chromosomes, assembly of new cellular structures, disassembly or remodelling of pre-existing organelles, distribution and reforming of organelles and cytoplasm, and remodelling of the plasma membrane. Membrane remodelling is crucial for the proper distribution of organelles and cytoplasm during division. The cell division cycle ensures the complete replication and segregation of chromosomal DNA into daughter cells. The process of cell division involves dynamic changes in the stress field exerted by dividing cells, with sequential changes in the localization and strength of traction stress. The structure of the cytoskeleton undergoes significant changes during cell division, with microtubules and actin-containing filaments disassembling into subunits or small oligomers, while intermediate filaments collapse into a meshwork associated with the nuclear membrane. Protein phosphorylation plays a role in timing the assembly of key components of the division machinery.
At what age cell division stops?8 answers
How to accelerate cell division?10 answers
What is the best study material for mitotic cell division?16 answers
What nutrients help cell division?10 answers

See what other people are reading

What are the effects of culture on gene expression of primary human cells?
4 answers
Culture conditions significantly impact the gene expression of primary human cells. Studies on nasal epithelial cells demonstrated that different culture media and interfaces influenced the expression of drug transporter genes, with air-liquid interfaces and longer culture times favoring higher expression levels of specific transporters. Similarly, research on alveolar epithelial type 2 cells found distinct transcriptomic differences between primary cells and cultured progeny, highlighting the importance of in vitro models for studying lung health and disease. Furthermore, exposure of bronchial epithelial cells to cigarette smoke resulted in significant changes in gene expression, while electronic nicotine delivery systems had minimal effects on gene expression in the same cell model. These findings emphasize the necessity of considering culture conditions when studying gene expression in primary human cells.Why optical coherence tomography is a popular imaging technique?
5 answers
Optical coherence tomography (OCT) is a popular imaging technique due to its non-invasive nature, high resolution, and diverse applications across various medical fields. Initially used for eye length measurements and retinal imaging, OCT has evolved to provide detailed structural and functional information in real-time. Its ability to visualize internal microstructures in three dimensions with high axial resolution makes it invaluable for assessing retinal microanatomy and vasculature. Furthermore, OCT angiography (OCTA) enhances its functionality by enabling label-free imaging of vasculature down to the capillary level. Beyond ophthalmology, OCT is utilized in dermatology, neurology, and other specialties, showcasing its versatility and widespread adoption in clinical practice and research. The continuous technological advancements in OCT systems have further solidified its position as a standard imaging modality in various medical disciplines.Can one use air lipid interface to research responses in copd epithelial cells?
5 answers
Yes, the air-liquid interface (ALI) method can be utilized to study responses in COPD epithelial cells. This method involves culturing primary nasal and bronchial epithelial cells under conditions where the cells are exposed to air at the apical surface while being nourished from below by liquid media. ALI cultures have been instrumental in demonstrating differences in tight junction disruption and vulnerability to cigarette smoke extract (CSE) in COPD versus control groups. Furthermore, ALI cultures have shown that COPD epithelial cells have a diminished inflammatory response to CSE exposure, increased susceptibility to apoptosis, and reduced Toll-like receptor-4 expression, shedding light on important cellular mechanisms in COPD pathogenesis. By using ALI cultures, researchers can gain valuable insights into the responses of COPD epithelial cells to environmental toxins like cigarette smoke.How does drying affect electrode?
5 answers
The drying process significantly impacts electrode properties in lithium-ion batteries. Drying temperature plays a crucial role, affecting binder distribution within the electrode coating. Higher temperatures lead to uneven binder distribution, while lower temperatures hinder binder diffusion, both influencing electrode performance. Optimal drying temperatures can enhance electronic conductivity and reduce ionic resistivity, crucial for cell properties. Near-infrared (NIR) radiation during drying can increase drying speed, electrode temperature, and adhesion force, offering advantages over convective drying. High drying rates may induce binder migration, impacting binder distribution throughout the electrode, emphasizing the need for informative in situ metrologies to understand the drying dynamics better. Ultrasound acoustic-based techniques provide insights into the dynamic drying process, aiding in achieving a more controllable electrode manufacturing process.How is nucleases used in the manufacturing of virus and viral vectors?
5 answers
Nucleases play a crucial role in the manufacturing of viruses and viral vectors by aiding in various processes. They are utilized for different purposes such as reducing host cell DNA presence during virus culturing, introducing nucleases into cells through lentiviral vector particles for gene targeting, and incorporating nuclease grafted adeno-associated viruses to prevent genotoxicity from prolonged expression of gene editing systems. Additionally, polypeptides with nuclease activity, generated through advanced methods like gene site mutagenesis and tailored multi-site combinatorial assembly, are also employed in the manufacturing process. Furthermore, self-limiting viral vectors containing genes encoding site-specific endonucleases are designed to cleave the vector in cells, limiting its persistence time and enabling targeted modifications of genetic sequences.What is typical Starch content of poaceae leaves in percentages?
5 answers
The starch content in Poaceae leaves typically ranges from 6% to 37%. Starch, a crucial storage carbohydrate, shows variations in content among different plant species. Poaceae, commonly known as grasses, exhibit distinct starch grain (SG) morphologies in their endosperm, with SG types classified into compound grains, bimodal simple grains, uniform simple grains, and mixed configurations. Evolutionary studies comparing grasses and dicots reveal differences in gene duplication, loss patterns, and evolutionary rates in the starch biosynthetic pathway, with grasses showing higher evolutionary rates and less gene loss, contributing to their higher starch content. Monitoring starch content in leaves using Raman microscopy can aid in predicting biomass production and enhancing breeding programs. A specific enzyme method allows for accurate estimation of starch in small quantities of leaf tissue, providing reliable results comparable to iodine-precipitation methods.What is the 2theta angle of xrd crystalline structure of metallic Fe ?
5 answers
The X-ray diffraction (XRD) analysis of metallic Fe typically shows diffraction peaks at specific 2θ angles, providing information about its crystalline structure. While the specific 2θ angle for metallic Fe is not explicitly mentioned in the provided contexts, it is crucial to note that XRD is a powerful tool for characterizing crystalline materials like Fe, providing insights into crystal orientation, lattice parameters, and phases present in the sample. XRD patterns of metallic Fe can reveal details about its atomic structure, grain size, and crystal defects, aiding in material characterization and analysis. Therefore, conducting XRD analysis on metallic Fe can offer valuable information about its crystalline properties, aiding in various industrial applications and material science research.Does microglia presence accelerate infection kinetics in primary cultures?
5 answers
Microglia presence in primary cultures can impact infection kinetics. While a study by Rawat et al. demonstrated that monocyte-derived microglia (MMG) infected with HIV showed similar replication kinetics to primary human microglia (HMG), another study by Cadiz et al. highlighted that cultured microglia undergo significant transcriptional changes, leading to an activated phenotype not reflective of in vivo microglia. Additionally, Tewari et al. showed that human microglia in defined culture conditions exhibited characteristics expected of native cells, including response to bacterial stimuli. Therefore, the presence of microglia in primary cultures can both mirror and deviate from in vivo conditions, impacting infection kinetics depending on the specific context and experimental setup.What is typical cellulose, xylan, pectin, xyloglucan, starch, and inulin content of poaceae leaves in percentages?
5 answers
The typical composition of various polysaccharides in Poaceae leaves can vary. Cellulose content in primary plant cell walls ranges from 9-25%. Xylan is a component of hemicelluloses, constituting 25-50% of the primary cell wall. Pectin, another primary cell wall component, makes up 10-35% of the cell wall. Xyloglucans, which attach to cellulose microfibrils, are present in varying amounts. Starch content in leaves can be monitored using Raman microscopy, aiding in biomass predictions. In Poaceae, the intragenomic base composition heterogeneity analysis revealed distinct base composition patterns, aiding in taxonomic classification within the family. The specific percentages of starch and inulin in Poaceae leaves were not directly provided in the contexts.What techniques are implemented to establish an adherent cell culture model for viral infection?
4 answers
To establish adherent cell culture models for viral infection, various techniques have been implemented. These include utilizing nanoself-assembled peptides as culture scaffolds for three-dimensional (3D) cell cultures, employing bioprinted 3D matrices for tissue-like growth characteristics and virus detection, adhering viral infected cells to fiber material supports in a culture vessel while controlling the culture medium, and developing scaffold-free human induced pluripotent stem cell-based adherent 3D neuronal cultures for modeling pathogen-central nervous system interactions and drug screening. Additionally, normal airway epithelial cells have been successfully cultured in 2D and 3D systems using conditional reprogramming and air-liquid interface techniques, providing a physiological model for studying emerging virus infections.How does the expression varies between knock out and Knock down any cell line?
5 answers
Protein expression in cell lines can vary between knock out and knock down approaches. Knock out of specific genes, like OAZ1, can lead to up to four-fold higher protein expression in HEK293 cells without affecting growth. In contrast, knock down of genes, such as p21, can show similar growth responses to knock out but with distinct gene expression profiles. Knock out strategies using CRISPR-Cas9 can efficiently generate knock-out cancer cell lines by inducing homozygous deletions, preventing unexpected gene expression variations seen in knock down approaches. These findings highlight the importance of considering the differences in protein expression outcomes between knock out and knock down methods when studying gene function in cell lines.