Why do we check antioxidant activity?
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| Its antioxidant activity can be responsible for its pharmacological effect and make it an attractive natural antioxidant. | |
572 Citations | Phenolic compounds are thus a major contributor of antioxidant activity. |
01 Feb 2005 17 Citations | 1 clearly demonstrates variations in antioxidant activity of various extracts |
| In our opinion, this method can be useful for estimating the antioxidant activity in lipophilic samples and as a complement to other methods that measure antioxidant activity in aqueous media. | |
27 Citations | The antioxidant activity would be mainly related to the intermediate and especially the final stage. |
105 Citations | The results question the validity of many existing methods of testing antioxidant activity. |
Related Questions
What is the significance of DPPH determination in antioxidant research?4 answersThe significance of DPPH determination in antioxidant research lies in its ability to assess antioxidant activity through radical scavenging capabilities. DPPH, a stable free radical, reacts with antioxidants, allowing for the evaluation of their effectiveness in neutralizing free radicals. Different solvents can influence the reactivity of antioxidants with DPPH, showcasing varying antioxidant capacities. By utilizing DPPH in methods like RP-HPLC and kinetic modeling, researchers can simultaneously evaluate multiple antioxidants, identify reaction products, and understand reaction kinetics, providing a comprehensive analysis of antioxidant activity. This method is crucial in determining the antioxidant potential of various compounds, such as plant extracts and food products, aiding in the development of antioxidant-rich formulations for health benefits.
What are the different ways that antioxidants can be determined?5 answersAntioxidants can be determined using various methods. Spectrophotometric methods, such as the DPPH assay, the ABTS assay, and the N,N-dimethyl-p-phenylenediamine (DMPD) assay, are commonly used for measuring antioxidant activity. Other spectrometry techniques, such as the Oxygen Radical Absorption Capacity (ORAC) test, the Hydroxyl Radical Antioxidant Capacity (HORAC) test, the Total Peroxyl Radical Trapping Antioxidant Parameter (TRAP) test, and the Total Oxyradical Scavenging Capacity (TOSC) test, are based on the transfer of a hydrogen atom or an electron. Chromatographic methods, combined with chemometric approaches, can also be used to identify and predict the antioxidant activity of herbal products. Electrochemical techniques, such as voltammetry, amperometry, biamperometry, and potentiometry, using carbon-based electrodes, are employed for determining antioxidant activity and individual antioxidants. Overall, there are a variety of methods available for determining the antioxidant activity of compounds, including spectrophotometric, chromatographic, and electrochemical techniques.
What is the rationale for using this assay to assess antioxidant activity?5 answersThe rationale for using assays to assess antioxidant activity is to determine the ability of substances to prevent or delay oxidation and the formation of free radicals. Antioxidants play a crucial role in maintaining cellular health and preventing the development of diseases caused by oxidative stress. Various methods have been developed to measure antioxidant capacity, including electron transfer-based assays, hydrogen atom transfer-based assays, and lipid peroxidation assays. These assays provide valuable information about the antioxidant potential of molecules or extracts and can be used to evaluate their efficacy in scavenging free radicals and protecting cellular structures. Additionally, assays such as the Vesicle Conjugated Autoxidizable Triene (VesiCAT) assay offer insights into the distribution and interaction of antioxidants with reactive oxygen species in membrane models. By using multiple assays with different mechanisms, the full capability of an antioxidant sample can be revealed.
How to determine the antioxidant activity of a crude extract using the DPPH assay?3 answersThe antioxidant activity of a crude extract can be determined using the DPPH assay. The DPPH method involves measuring the ability of the extract to scavenge the DPPH radical, which is a stable free radical. The assay is performed by adding the extract to a DPPH solution and measuring the decrease in absorbance at a specific wavelength using UV-VIS spectrophotometry. The antioxidant activity is expressed as the IC50 value, which is the concentration of the extract required to scavenge 50% of the DPPH radicals. A lower IC50 value indicates a higher antioxidant activity. The DPPH assay has been used to determine the antioxidant properties of various plant extracts, including Javanese bark, mentha and guggul leaves, Croatian and Greek watermelon seeds, and young coconut shell and coir.
What are the main methods of measuring oxidative stress?5 answersThe main methods of measuring oxidative stress include measuring free radicals and active species by flow cytometry, determining the total antioxidant capacity of body fluids, measuring the oxidation products of macromolecules, determining the activity of antioxidant enzymes, and changing the expression of genes related to the antioxidant system. Another method involves inducing all blood cells to produce excessive reactive oxygen species (ROS) and measuring the fluorescence or color intensity of a dye that reacts with ROS. Additionally, a method for quantitatively measuring oxidative stress involves quantifying the level of oxidative stress using an influence factor. Other techniques include using specific biomarkers and probes to detect reactive species in cells and measuring oxidative species through lipid peroxidation, protein oxidation, and activity of antioxidant enzymes. Furthermore, a means for measuring reactive oxygen in the human body involves using a color indicator that undergoes a color change upon reaction with malondialdehyde, allowing for the quantitative detection of reactive oxygen.
How are antioxidants tested in the body?7 answers