Topic
2,3-Butanediol
About: 2,3-Butanediol is a(n) research topic. Over the lifetime, 299 publication(s) have been published within this topic receiving 6016 citation(s). The topic is also known as: Pseudobutylene glycol & Dimethylene glycol.
Papers published on a yearly basis
Papers
More filters
TL;DR: At any of the pH values tested, excess glycerol in the culture enhanced the butanediol yield, and the undissociated acid being a stronger trigger than the less toxic diols and acid anions.
Abstract: Klebsiella pneumoniae was shown to convert glycerol to 1,3-propanediol, 2,3-butanediol and ethanol under conditions of uncontrolled pH. Formation of 2,3-butanediol starts with some hours' delay and is accompanied by a reuse of the acetate that was formed in the first period. The fermentation was demonstrated in the type strain of K. pneumoniae, but growth was better with the more acid-tolerant strain GT1, which was isolated from nature. In continuous cultures in which the pH was lowered stepwise from 7.3 to 5.4, 2,3-butanediol formation started at pH 6.6 and reached a maximum yield at pH 5.5, whereas formation of acetate and ethanol declined in this pH range. 2,3-Butanediol and acetoin were also found among the products in chemostat cultures grown at pH 7 under conditions of glycerol excess but only with low yields. At any of the pH values tested, excess glycerol in the culture enhanced the butanediol yield. Both effects are seen as a consequence of product inhibition, the undissociated acid being a stronger trigger than the less toxic diols and acid anions. The possibilities for using the fermentation type described to produce 1,3-propanediol and 2,3-butanediol almost without by-products are discussed.
217 citations
TL;DR: The production of 2,3-butanediol by bacterial species continues to be of great interest because of its varied application as discussed by the authors, and two bacterial species, Bacillus polymyxa and Klebsiella pneumoniae have demonstrated potential for butanediol fermentation on a commercial scale.
Abstract: The production of 2,3-butanediol by bacterial species continues to be of great interest because of its varied application. Two bacterial species, Bacillus polymyxa and Klebsiella pneumoniae have demonstrated potential for butanediol fermentation on a commercial scale. Klebsiella pneumoniae, owing to its broad substrate and cultural adaptability, is the most thoroughly investigated organism. It is generally recommended that natural resources of ‘waste’ cellulosic and hemicellulosic substrates be fermented, for the improvement of process economics. The process of diol fermentation is reportedly influenced by various nutritional and environmental parameters. The high boiling-point characteristic of 2,3-butanediol poses serious problems in recovery of diol from fermented slurry. Repeated solvent extraction and countercurrent-stream stripping have been recommended as the methods of choice for diol recovery.
210 citations
TL;DR: Since both types of bacteria can not be affiliated to any of the existing genera and species, the new species Pelobacter carbinolicus and Pelobacteria propionicus are proposed.
Abstract: From anaerobic enrichments with 2,3-butanediol as sole substrate pure cultures of new Gram-negative, strictly anaerobic, non-sporeforming bacteria were isolated. Similar isolates were obtained with acetoin as substrate. From marine muds in saltwater medium a short rod (strain Gra Bd 1) was isolated which fermented butanediol, acetoin and ethylene glycol to acetate and ethanol. The DNA base ratio of this strain was 52.3 mol% guanine plus cytosine.
144 citations
TL;DR: The effects of pH, xylose concentration, and the oxygen transfer rate on the bioconversion of D‐xylose to 2,3‐butanediol are described.
Abstract: It is known that 2,3-butanediol is a potentially valuable chemical feedstock that can be produced from the sugars present in hemicellulose and celluose hydrolysates. Klebsiella oxytoca is able to ferment most pentoses, hexoses, and disaccharides. Butanediol appears to be a primary metabolite, excreted as a product of energy methabolism. The theoretical maximum yield of butanediol from monosaccharides is 0.50 g/g. This article describes the effects of pH, xylose concentration, and the oxygen transfer rate on the bioconversion of D-xylose to 2,3-butanediol. Product inhibition by butanediol is also examined. The most important variable affecting the kinetics of this system appears to be the oxygen transfer rate. A higher oxygen supply favors the formation of cell mass at the expense of butanediol. Decreasing the oxygen supply rate increases the butanediol yield, but decreases the overall conversion rate due to a lower cell concentration.
142 citations
TL;DR: The results suggested that inactivation of aldA led to a significantly improved 2,3-BD production.
Abstract: Ethanol was a major byproduct of 2,3-butanediol (2,3-BD) fermentation by Klebsiella oxytoca ME-UD-3. In order to achieve a high efficiency of 2,3-BD production, K. oxytoca mutants deficient in ethanol formation were successfully constructed by replace the aldA gene coding for aldehyde dehydrogenase with a tetracycline resistance cassette. The results suggested that inactivation of aldA led to a significantly improved 2,3-BD production. The carbon flux to 2,3-BD was enhanced by eliminating the byproducing ethanol and at the same time reducing the accumulation of another byproduct acetoin. At last, by fed-batch culturing of the mutant, the final 2,3-BD titer up to 130 g/l with the productivity of 1.63 g/l.h and the 2,3-BD yield relative to glucose of 0.48 g/g was obtained.
121 citations