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Showing papers on "2,3-Butanediol published in 1946"



Journal ArticleDOI
TL;DR: Aeration by mechanical agitation of 15% wheat mash fermented by Aerobacillus polymyxa inhibited the formation of 2,3-butanediol and particularly of ethanol as discussed by the authors.
Abstract: Aeration by mechanical agitation of 15% wheat mash fermented by Aerobacillus polymyxa inhibited the formation of 2,3-butanediol and particularly of ethanol. Aeration of similar mashes by passage of finely dispersed air or oxygen at the rate of 333 ml. per minute per litre of mash increased the rate of formation and yield of 2,3-butanediol but inhibited ethanol formation. However, the over-all time required for the completion of fermentation was not shortened from the usual 72 to 96 hr. required for unaerated mashes. There was no evidence of a shift from fermentative to oxidative dissimilation. Under aerobic conditions, the final butanediol–ethanol ratio was approximately 3:1. Anaerobic conditions, as produced by the passage of nitrogen or hydrogen through the mash, increased the rate of formation of both butanediol and ethanol and shortened the fermentation time to about 48 hr. Under these conditions, the butanediol–ethanol ratio was reduced to about 1.3:1.0. Carbon dioxide gave a butanediol–ethanol ratio...

11 citations


Journal ArticleDOI
TL;DR: Yeast extract, casein hydrolysate, and a mixture of 13 to 20 ammo acids were found to be superior to simpler substances such as ammonium sulphate, urea, potassium nitrate, or asparagine as sources of nitrogen for Bacillus polymyxa in relation to production of 2,3-butanediol from starch as discussed by the authors.
Abstract: Yeast extract, casein hydrolysate, and a mixture of 13 to 20 ammo acids were found to be superior to simpler substances such as ammonium sulphate, urea, potassium nitrate, or asparagine as sources of nitrogen for Bacillus polymyxa in relation to production of 2,3-butanediol from starch The complex sources of nitrogen were more or less interchangeable for most, but not all strains of this organism with regard to effectiveness for diol production, but the addition of yeast extract to either casein hydrolysate or amino acids resulted in a slightly higher yield of diol and an increase in the diol/ethanol ratio from 2 to 2.6 or higher.The requirements of different strains for specific ammo acids varied somewhat, but the need for isoleucine and asparagine was common to the tour strains studied. A fairly good fermentation was obtained with the most efficient of these strains in a medium containing isoleucine, tyrosine, glycine, methionine, and asparagine after three days' incubation. The fermentation went to com...

3 citations


Journal ArticleDOI
TL;DR: Considerable quantities of acetone were produced by certain strains of Aerobacillus polymyxa growing anaerobically in a dextrose medium and the presence ofacetone was confirmed by formation of the 2,4-dinitrophenylhydrazine derivative.
Abstract: Considerable quantities of acetone were produced by certain strains of Aerobacillus polymyxa growing anaerobically in a dextrose medium. The presence of acetone was confirmed by formation of the 2,4-dinitrophenylhydrazine derivative. Carbon balances were obtained for both non-acetone-producing and acetone-producing strains of the organism; considerable quantities of several organic acids were also formed. Increased efficiency of nitrogen-aeration resulted in a marked lowering of the butanediol: ethanol ratio and probably is conducive to an increased yield of acetone.

3 citations