Showing papers on "2,3-Butanediol published in 1991"

The production of 2,3-butanediol by fermentation of high test molasses

Abstract: Klebsiella oxytoca fermented 199 g·l−1 high test or invert molasses using batch fermentation with substrate shift to produce 95.2–98.6 g 2,3-butanediol·l−1 and 2,4–4.3 g acetoin·l−1 with a diol yield of 96–100% of the theoretical value and a diol productivity of 1.0–1.1 g·l−1·h−1. Fermentation was performed numerous times with molasses in repeated batch culture with cell recovery. Such repeated batch fermentation, in addition to a high product yield, also showed a very high product concentration. For example, 118 g 2,3-butanediol·l−1 and 2.3 g acetoin·l−1 were produced from 280 g·l−1 of high test molasses. The diol productivity in this fermentation amounted to 2.4 g·l−1·h−1 and can undoubtedly be further increased by increasing the cell concentration. Because the Klebsiella cultures ferment 2,3-butanediol at an extremely high rate once the sugar has been consumed, the culture was inhibited completely by the addition of 15 g ethanol·l−1 and switching off aeration.

A model for multiproduct-inhibited growth of Enterobacter aerogenes in 2,3-butanediol fermentation

Abstract: Ethanol is identified as a strongly inhibitory metabolite in addition to acetic acid and 2,3-butanediol in 2,3-butanediol production by Enterobacter aerogenes. A model is proposed to describe the multiproduct-inhibited growth of E. aerogenes in 2,3-butanediol fermentation. The model is verified with data from anaerobic and microaerobic continuous culture. On the basis of this model the difference in biomass production and product patterns during anaerobic and microaerobic growth of E. aerogenes is discussed.