Showing papers on "2,3-Butanediol published in 2007"
TL;DR: In this article, the effect of pH, various carbohydrate source, initial glucose concentration and aeration on 2,3-butanediol fermentation by B. amyloliquefaciens were investigated.
Abstract: Most studies on 2,3-butanediol production by microbial fermentation have been carried out using Klebsiella oxytoca and Bacillus polymyxa. In these fermentations there is a significant conversion of carbon source into ethanol, glycerol and various organic acids as by-products. On the other hand, this investigation has shown that the major products of fermentation by Bacillus amyloliquefaciens are 2,3-butanediol and some minor acids. In a typical fermentation about 33 g of butanediol was produced from 100 g of glucose.
The effect of pH, various carbohydrate source, initial glucose concentration and aeration on the 2,3-butanediol fermentation by B. amyloliquefaciens were investigated. The butanediol productivity and yield was found to be highest with glucose as the carbohydrate source. The glucose concentration was varied between 30 and 100 g dm−3 with oxygen transfer rate controlled between 47.5 and 78.0 h−1. Oxygen and the initial substrate concentrations significantly influence the yield, productivity and by-product formation.
27 citations
Journal Article•
TL;DR: In this paper, the effects of several carbon sources,nitrogen sources,citric acid and initial concentration of sucrose on cell growth and 2,3-butanediol production by Serratia marcescens were investigated.
Abstract: Effects of several carbon sources,nitrogen sources,citric acid and initial concentration of sucrose on cell growth and 2,3-butanediol production by Serratia marcescens were investigated.The concentrations of medium components were optimized by the methods of one factor,orthogonal experiment and Box-Behnken design,and response surface analysis methods.Moreover,results showed that citric acid added in medium could improve cell growth and sugar utilization,reduce fermentation time and stimulate the production of 2,3-butanediol.It could accumulate 2,3-butanediol from 14.03 g/L up to 39.27 g/L.
2 citations
Patent•
20 Jun 2007
TL;DR: In this paper, a method for preparing 2, 3- butanediol, belonging to biological chemical industry technique field, is described, which comprises following steps: preparing culture medium, culturing seed and producing 2,3-butanediol; adding exterior addition factor into fermentation culture medium before fermentation; or adding external addition factor during bacteria exponent growth period in 2, three-butenediol producing process; said exterior addition factors is one or many of citrate, alpha- ketoglutaric acid, boletic acid and L- malic acid.
Abstract: The invention discloses a method for preparing 2, 3- butanediol, belonging to biological chemical industry technique field. It comprises following steps: preparing culture medium, culturing seed and producing 2, 3- butanediol; adding exterior addition factor into fermentation culture medium before fermentation; or adding exterior addition factor during bacteria exponent growth period in 2, 3- butanediol producing process; said exterior addition factor is one or many of citrate, alpha- ketoglutaric acid, boletic acid and L- malic acid. The invention is characterized in that it can increase substrate conversion rate, increase concentration and production strength for target product 2, 3- butanediol, the operation is simple, needs no exterior labor and device, and production cost is low.
2 citations