2,3-Butanediol

About: 2,3-Butanediol is a research topic. Over the lifetime, 299 publications have been published within this topic receiving 6016 citations. The topic is also known as: Pseudobutylene glycol & Dimethylene glycol.

Characterization of the Enzyme Involved in Formation of 2-Butanol from meso-2,3-Butanediol by Lactic Acid Bacteria

Abstract: Strains of Lactobacillus brevis originally isolated from spoiled wine reduced meso -2,3-butanediol to 2-butanol. This reaction was initiated by dehydration of the diol to methyl ethyl ketone, that acted as hydrogen acceptor during the joint fermentation of glucose to CO 2 , lactate, acetate, and ethanol. The diol dehydrase catalyzing the dehydration of meso -2,3-butanediol to methyl ethyl ketone was purified to electrophoretic homogeneity. The enzyme reacted with 1,2-propanediol, glycerol, and meso -2,3-butanediol. The corresponding K m values were 0.7 m M , 5.5 m M , and 34.0 m M , respectively. The dehydrase showed optimum activity at pH 7.0 and at 30°C. It was activated by ammonium and potassium ions and inhibited by lithium, sodium, magnesium, and manganese ions. The meso -butanediol dehydrase showed the same characteristics as the diol dehydrase from Lactobacillus brevis that has been isolated previously and is obviously identical to this enzyme. The meso -2,3-butanediol dehydrase had a relative molecular weight (M r ) of about 230 000 daltons and consisted of four different subunits.

Production of 2,3-Butanediol from Sucrose by a Klebsiella Species

Abstract: Chemical 2,3-butanediol is an important platform compound possessing diverse industrial applications. So far, it is mainly produced by using petrochemical feedstock which is associated with high cost and adverse environmental impacts. Hence, finding alternative routes (e.g., via fermentation using renewable carbon sources) to produce 2,3-butanediol are urgently needed. In this study, we report a wild-type Klebsiella sp. strain XRM21, which is capable of producing 2,3-butanediol from a wide variety of carbon sources including glucose, sucrose, xylose, and glycerol. Among them, fermentation of sucrose leads to the highest production of 2,3-butanediol. To maximize the production of 2,3-butanediol, fermentation conditions were first optimized for strain XMR21 by using response surface methodology (RSM) in batch reactors. Subsequently, a fed-batch fermentation strategy was designed based on the optimized parameters, where 91.2 g/L of 2,3-butanediol could be produced from substrate sucrose dosing in 100 g/L for three times. Moreover, random mutagenesis of stain XMR21 resulted in a highly productive mutant strain, capable of producing 119.4 and 22.5 g/L of 2,3-butanediol and ethanol under optimized fed-batch fermentation process within 65 h with a total productivity of 2.18 g/L/h, which is comparable to the reported highest 2,3-butanediol concentration produced by previous strains. This study provides a potential strategy to produce industrially important 2,3-butanediol from low-cost sucrose.

Synthesis of All Four Possible Stereoisomers of 1-Phenyl-2, 3-butanediol and Both Enantiomers of 3-Hydroxy-4-phenyl-2-butanone to Determine the Absolute Configuration of the Natural Constituents

Abstract: Enantioselective syntheses of all the possible stereoisomers of 1-phenyl-2,3-butanediol (erythro isomer 1 and threo isomer 2) and of 3-hydroxy-4-phenyl-2-butanone 3, the odor components of wisteria flowers, was accomplished via Sharpless asymmetric epoxydation. The absolute configurations of 1–3 were determined by an HPLC analysis of the corresponding MTPA esters of synthetic samples.