2,3-Butanediol

About: 2,3-Butanediol is a research topic. Over the lifetime, 299 publications have been published within this topic receiving 6016 citations. The topic is also known as: Pseudobutylene glycol & Dimethylene glycol.

Metabolic engineering of Klebsiella pneumoniae and in silico investigation for enhanced 2,3-butanediol production.

Abstract: To improve the production of 2,3-butanediol (2,3-BD) in Klebsiella pneumoniae, the genes related to the formation of lactic acid, ethanol, and acetic acid were eliminated. Although the cell growth and 2,3-BD production rates of the K. pneumoniae ΔldhA ΔadhE Δpta-ackA strain were lower than those of the wild-type strain, the mutant produced a higher titer of 2,3-BD and a higher yield in batch fermentation: 91 g 2,3-BD/l with a yield of 0.45 g per g glucose and a productivity of 1.62 g/l.h in fed-batch fermentation. The metabolic characteristics of the mutants were consistent with the results of in silico simulation. K. pneumoniae knockout mutants developed with an aid of in silico investigation could produce higher amounts of 2,3-BD with increased titer, yield, and productivity.

Production of High Levels of Chirally Pure d-2,3-Butanediol with a Newly Isolated Bacillus Strain

Abstract: 2,3-Butanediol (2,3-BD), especially when it exists in the chirally pure form, is a valuable chemical feedstock with numerous applications in various industries However, in most cases, the 2,3-BD naturally produced by the microorganism is a mixture of two of the three isomers (d-2,3-BD, l-2,3-BD, and meso-2,3-BD), which has restricted applications and thus a much lower value than the chirally pure one In this study, we report a new isolate Bacillus spp FJ-4 strain, which can produce highly chirally pure d-2,3-BD (>999%) to high levels, with very high productivity and yield The composition of the fermentation medium for the strain was sequentially optimized using statistical experimental methodologies During a fed-batch experiment, 1000 g/L of d-2,3-BD was produced from 2268 g/L glucose, with a yield of 044 g/g (882% of the theoretical maximum) Acetoin was the primary byproduct, and no other byproduct such as lactate, acetate, or ethanol was detected, which would be advantageous for the downstrea