Topic
2,3-Butanediol
About: 2,3-Butanediol is a research topic. Over the lifetime, 299 publications have been published within this topic receiving 6016 citations. The topic is also known as: Pseudobutylene glycol & Dimethylene glycol.
Papers published on a yearly basis
Papers
More filters
Journal Article•
TL;DR: In this paper, the effects of several carbon sources,nitrogen sources,citric acid and initial concentration of sucrose on cell growth and 2,3-butanediol production by Serratia marcescens were investigated.
Abstract: Effects of several carbon sources,nitrogen sources,citric acid and initial concentration of sucrose on cell growth and 2,3-butanediol production by Serratia marcescens were investigated.The concentrations of medium components were optimized by the methods of one factor,orthogonal experiment and Box-Behnken design,and response surface analysis methods.Moreover,results showed that citric acid added in medium could improve cell growth and sugar utilization,reduce fermentation time and stimulate the production of 2,3-butanediol.It could accumulate 2,3-butanediol from 14.03 g/L up to 39.27 g/L.
2 citations
Patent•
18 Jul 2017
TL;DR: A modified strain of the bacterium Lactococcus lactis obtainable by a method that comprises a step of fusion of two protoplasts from two parent strains which, compared to the wild type strain from which they derive, show an increased ability to produce acetoin and/or 2,3 butanediol (2,3-BDO).
Abstract: The present invention provides a modified strain of the bacterium Lactococcus lactis obtainable by a method that comprises a step of fusion of two protoplasts from two Lactococcus lactis parental strains which, compared to the wild type strain of Lactococcus lactis from which they derive, show: (a) an increased ability to produce acetoin and/or 2,3- butanediol (2,3-BDO), and (b) a decreased ability to produce lactic acid, when cultured under aerobic conditions, and wherein the modified strain of Lactococcus lactis has an increased ability to produce 2,3-BDO of at least 20 times the amount produced by the wild type strain, an increased ability to produce acetoin of at least 20 times the amount produced by the wild type strain and a decreased ability to produce lactic acid of at least 10 times the amount produced by the wild type strain, when cultured under aerobic conditions. Also provided are methods to produce acetoin and 2,3- BDO..
2 citations
TL;DR: In this paper , dead Cas9 (dCas9) was used to reduce ethanol synthesis during 2,3-butanediol production without reduction of glucose, and the binding site of guide RNA used to effectively suppress PDC1 promoter-driven red fluorescent protein expression by dCas9 was identified and applied to control PDC 1 expression.
2 citations
Patent•
29 Jun 2016
TL;DR: In this article, a preparation method of 2,3-butanediol through microbial fermentation is presented, which can effectively remove residual glucose in the fermentation liquid, and is in favor of implementation of next-step twoaqueous-phase extraction, rectification and other refining ways.
Abstract: The invention discloses a preparation method of 2,3-butanediol through microbial fermentation; the method includes the steps: (1) strain culture: culturing lactobacillus with an MRS culture medium, to obtain a lactobacillus seed liquid; and culturing klebsiella pneumoniae with an LB culture medium, to obtain a klebsiella pneumoniae seed liquid; (2) fermentation culture: inoculating a fermentation culture medium with the klebsiella pneumoniae seed liquid, carrying out micro-aerobic fermentation, batch-feeding a glucose solution in the fermentation process to control the concentration of glucose in the fermentation system to be 20 g/L-40 g/L; and (3) fermentation control: in a fermentation last stage, adjusting the pH of the fermentation liquid to 5-6, inoculating with the lactobacillus seed liquid, and carrying out aerobic fermentation under a low-pH condition. The method can effectively remove residual glucose in the fermentation liquid, and is in favor of implementation of next-step two-aqueous-phase extraction, rectification and other refining ways, and is suitable for mass production and application.
2 citations
Patent•
20 Jun 2007
TL;DR: In this paper, a method for preparing 2, 3- butanediol, belonging to biological chemical industry technique field, is described, which comprises following steps: preparing culture medium, culturing seed and producing 2,3-butanediol; adding exterior addition factor into fermentation culture medium before fermentation; or adding external addition factor during bacteria exponent growth period in 2, three-butenediol producing process; said exterior addition factors is one or many of citrate, alpha- ketoglutaric acid, boletic acid and L- malic acid.
Abstract: The invention discloses a method for preparing 2, 3- butanediol, belonging to biological chemical industry technique field. It comprises following steps: preparing culture medium, culturing seed and producing 2, 3- butanediol; adding exterior addition factor into fermentation culture medium before fermentation; or adding exterior addition factor during bacteria exponent growth period in 2, 3- butanediol producing process; said exterior addition factor is one or many of citrate, alpha- ketoglutaric acid, boletic acid and L- malic acid. The invention is characterized in that it can increase substrate conversion rate, increase concentration and production strength for target product 2, 3- butanediol, the operation is simple, needs no exterior labor and device, and production cost is low.
2 citations