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2,3-Butanediol

About: 2,3-Butanediol is a research topic. Over the lifetime, 299 publications have been published within this topic receiving 6016 citations. The topic is also known as: Pseudobutylene glycol & Dimethylene glycol.


Papers
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Journal ArticleDOI
TL;DR: In this paper , a thermotolerant xylose-utilizing microbes were isolated from 6 different districts in Laos and screened for a low accumulation of xylitol in a xyloses medium at 37 ˚C. One isolate was found to produce 2,3-BD and identified by 16S rDNA sequencing.
Abstract: 2,3-Butanediol (2,3-BD), a valuable compound used for chemicals, cosmetics, pesticides and pharmaceuticals, has been produced by various microbes. However, no high-temperature fermentation of the compound at high productivity has been reported.Thermotolerant xylose-utilizing microbes were isolated from 6 different districts in Laos and screened for a low accumulation of xylitol in a xylose medium at 37 ˚C. One isolate was found to produce 2,3-BD and identified by 16S rDNA sequencing. The 2,3-BD fermentation capacity was investigated at different temperatures using xylose and glucose as carbon sources, and the fermentation parameters were determined by a high-performance liquid chromatography system.By screening for a low accumulation of xylitol in a xylose medium, one isolate that accumulated almost no xylitol was obtained. Further analyses revealed that the isolate is Cronobacter sakazakii and that it has the ability to produce 2,3-BD at high temperatures. When xylose and glucose were used, this strain, named C. sakazakii OX-25, accumulated 2,3-BD in a short period before the complete consumption of these sugars and then appeared to convert 2,3-BD to acetoin. The optimum temperature of the 2,3-BD fermentation was 42 ˚C to 45 ˚C, and the maximum yield of 2,3-BD was 0.3 g/g at 12 h in 20 g/l xylose medium and 0.4 g/g at 6 h in 20 g/l glucose medium at 42 ˚C. The 2,3-BD productivity of the strain was higher than the 2,3-BD productivities of other non-genetically engineered microorganisms reported previously, and the highest productivity was 0.6 g/l·h and 1.2 g/l·h for xylose and glucose, respectively.Among thermotolerant microbes isolated in Laos, we discovered a strain, C. sakazakii OX-25, that can convert xylose and glucose to 2,3-BD with high efficiency and high productivity at high temperatures, suggesting that C. sakazakii OX-25 has the potential for industrial application to produce 2,3-BD as an important platform chemical.

2 citations

Patent
23 Nov 2016
TL;DR: In this paper, an engineering bacterium lacking organic acid production paths and use thereof in co-production of 1, 3-propanediol, 2,3-butanediol and ethanol was revealed.
Abstract: The invention discloses an engineering bacterium lacking organic acid production paths and use thereof in co-production of 1,3-propanediol, 2,3-butanediol and ethanol. Lactic dehydrogenase gene of lactic acid synthesis path of Klebsiella, acetate kinase gene and pyruvic oxidase gene in acetic acid synthesis path, and fumarate reductase gene and isocitrate lyase gene of succinic acid synthesis path are inactivated, so that production of organic acids in metabolism of glycerol of the Klebsiella can be reduced, and yield and conversion rate of alcohols such as1,3-propanediol, 2,3-butanediol and ethanol can be improved, and the co-production of the 1,3-propanediol, 2,3-butanediol and ethanol can be achieved. As a significant reduction in the production of the organic acids, the extraction process of the alcohols can be simplified. The production efficiency of production of high value products by microbial conversion of glycerol can be improved, the production cost is reduced, and the engineering bacterium has important application value.

2 citations

Journal ArticleDOI
TL;DR: A microbial contribution to the phenotypic characteristic of Fmo5 −/− mice of decreased plasma cholesterol is indicated and 2,3-butanediol is identified as a potential agent for lowering plasma cholesterol.
Abstract: We previously showed that Fmo5 −/− mice exhibit a lean phenotype and slower metabolic ageing. Their characteristics include lower plasma glucose and cholesterol, greater glucose tolerance and insulin sensitivity, and a reduction in age-related weight gain and whole-body fat deposition. In this paper, nuclear magnetic resonance (NMR) spectroscopy-based metabolite analyses of the urine of Fmo5 −/− and wild-type mice identified two isomers of 2,3-butanediol as discriminating urinary biomarkers of Fmo5 −/− mice. Antibiotic-treatment of Fmo5 −/− mice increased plasma cholesterol concentration and substantially reduced urinary excretion of 2,3-butanediol isomers, indicating that the gut microbiome contributed to the lower plasma cholesterol of Fmo5 −/− mice, and that 2,3-butanediol is microbially derived. Short- and long-term treatment of wild-type mice with a 2,3-butanediol isomer mix decreased plasma cholesterol and epididymal fat deposition but had no effect on plasma concentrations of glucose or insulin, or on body weight. In the case of long-term treatment, the effects were maintained after withdrawal of 2,3-butanediol. Short-, but not long-term treatment, also decreased plasma concentrations of triglycerides and non-esterified fatty acids. Fecal transplant from Fmo5 −/− to wild-type mice had no effect on plasma cholesterol, and 2,3-butanediol was not detected in the urine of recipient mice, suggesting that the microbiota of the large intestine was not the source of 2,3-butanediol. However, 2,3-butanediol was detected in the stomach of Fmo5 −/− mice, which was enriched for Lactobacillus genera, known to produce 2,3-butanediol. Our results indicate a microbial contribution to the phenotypic characteristic of Fmo5 −/− mice of decreased plasma cholesterol and identify 2,3-butanediol as a potential agent for lowering plasma cholesterol.

2 citations

Patent
19 May 2010
TL;DR: In this article, a method for producing 2,3-butanediol by the fermentation of enterobacter sp.Y5 is presented. But the method requires a simple fermentation process and short fermentation period.
Abstract: The invention discloses a method for producing 2,3-butanediol by the fermentation of enterobacter sp.Y5. A fermentation culture medium comprises the following components: 18-24g/L of glucose, 4-6g/L of tryptone, 2-4g/L of beef extract, 1-2g/L of yeast powder, 3-5g/L of NaCl, 1-3g/L of KH2PO4, 0.1-0.3g/L of MgSO4, 0.3-1.0g/L of L-cysteine and 8-15ml of trace element liquid. The enterobacter sp.Y5 is subject to seed culture and fermentation culture to obtain the 2,3-butanediol. The invention has simple fermentation process and short fermentation period.

2 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202328
202235
202110
20207
201911
201815