Showing papers on "Acacetin published in 2006"

Xanthine oxidase inhibitors from the flowers of Chrysanthemum sinense.

Abstract: From the MeOH extract of the flowers of Chrysanthemum sinense, a new flavone glucoside, acacetin 7- O-(3- O-acetyl- beta- D-glucopyranoside), has been isolated together with 27 known compounds including flavonoids, caffeoylquinic acid derivatives, phenolics, and a monoterpenoid glucoside. Their structures were elucidated on the basis of spectroscopic data. Compounds and displayed significant xanthine oxidase inhibitory activity in a concentration-dependent manner, and compounds and showed more potent inhibitory activity, with IC50 values ranging from 0.13 to 2.31 microM, than that of a positive control allopurinol (IC50=2.50 microM). The kinetic study indicated that and displayed competitive-type inhibition like that of allopurinol, while displayed a mixed-type inhibition.

Chrozophorin: a new acylated flavone glucoside from Chrozophora tinctoria (Euphorbiaceae)

Abstract: Preparative reversed-phase HPLC analysis of the methanol extract of the aerial parts of Chrozophora tinctoria (Euphorbiaceae) yielded five flavonoid glycosides, quercetin 3-O-rutinoside (1, rutin), acacetin 7-O-rutinoside (2), apigenin 7-O-b-D-[(6-p-coumaroyl)]-glucopyranoside (3), apigenin 7-O- b-D-glucopyranoside (4) and apigenin 7-O-b-D-[6-(3,4-dihydroxybenzoyl)]-glucopyranoside (named, chrozophorin, 5), the last one being a new natural product. The structures of these compounds were elucidated unambiguously by UV spectroscopic analyses using shift reagents, ESIMS, and 1D and 2D NMR spectroscopic techniques. The free-radical scavenging activity of the methanol extract (RC50 = 2.24 x 10-1 mg/mL) as well as the isolated compounds (1-5) (RC50 = 4.38 x 10-3, 2.26 x 10-2, 7.69 x 10-4, 8.71 x 10-3 and 3.19 x 10-4 mg/mL, respectively) were assessed by the DPPH assay.

Isolation and HPLC quantitative analysis of antioxidant flavonoids from Alternanthera tenella Colla.

Abstract: Phytochemical analysis of the antioxidant ethanolic extract of Alternanthera tenella Colla led to the isolation of six flavonoids, acacetin 8-C-[alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranoside] (1), 2"-O-alpha-L-rhamnopyranosyl-vitexin (2), 2"-O-beta-D-glucopyranosyl-vitexin (3), vitexin (4), quercetin (5) and kaempferol (6). All the structures were established by ESI-MS and NMR spectroscopic methods. Antioxidant capacity of extract, fractions and isolated compounds was determined using the oxygen radical absorbance capacity (ORAC) assay and extract, fractions and flavonoids isolated showed antioxidant activity in vitro. Moreover, the total soluble phenolic contents of the extract and fractions were measured using the Folin-Ciocalteau reagent and the quantitative analysis of flavone C-glycosides major constituents was performed by HPLC.

Cytotoxic Flavonoids from the Whole Plants of Chrysanthemum zawadskii Herbich var. latilobum Kitamura

Abstract: Two known flavonoids, luteolin (1) and acacetin (2) were isolated from a CHCl₃ soluble fraction of the whole plants of Chrysanthemum zawadskii Herbich var. latilobum Kitamura, and their structures were determined by NMR analysis. The luteolin (1) was isolated from this plant for the first time. These compounds were examined for their in vitro cytotoxic activities against four human cancer cell lines including HCT116 (colon), UO-31 (renal), PC-3 (prostate) and A549 (lung) by sulforhodamine B(SRB) assay. Acacetin (2) showed significant cytotoxic activity against HCT116 and UO-31 cells with an IC?? of 2.44 and 2.89 μg/ ml, respectively.

Studies on flavonoid constituents from herbs of Artemisia ordosica II

Abstract: Objective To study the chemical constituents of herbs of Artemisia ordosica. Method The chemical constituents were isolated and repeatedly purified on silica gel column and the structures were elucidated by the NMR spectra and physicochemical properties. Result Eight flavones were obtained and they were identified as isosakuranetin, 7, 4'-dimethylaro madendrin, acacetin, cirsimaritin, rhamnetin, eupatolin, 5, 7, 2', 4'-tetrahydroxy-6, 5'-dimethoxyflavone, hyperoside. Conclusion All the compounds were obtained from herbs of A. ordosica for the first time.

Pentadecanoic acid β-D-glucoside from Clerodendrum inerme

Abstract: A new aliphatic glucoside, isolated from the hexane extract of aerial parts of Clerodendrum inerme, has been characterized as pentadecanoic acid-p-D-glucoside, on the basis of spectral data analyses and chemical studies. Stigmasterol glucoside was also isolated from the same extract for the first time from this plant species. The butanol extract afforded acacetin and apigenin.

Chemical Constituents from Chrysanthemum morifolium Ramat

Abstract: By silica gel and Sephadex LH-20 column chromatography methods,fourteen compounds were isolated from ethanolic extracts of Chrysanthemum morifolium Ramat and identified as chrysoeriol(1),luteolin(2),apigenin(3),chrysoeriol 7-O-β-D-glucopyranoside(4),luteolin 7-O-β-D-glucopyranoside(5),acacetin 7-O-β-D-glucopyranoside(6),acacetin 7-O-β-D-glucopyranosyl(6→1)-α-L-rhamnopyranoside(7),acacetin 7-O-(6″-O-acetyl)-β-D-glucopyranoside(8),acacetin(9),kaempferol(10),eupatilin(11),emodin(12),chrysophanol(13) and physcion(14).Among them,compounds 8～14 were isolated from the plant for the first time.

Protection against DNA damage and inhibition of lipid peroxidation by flavones from Eremosparton songoricum (Litv) Vass

Abstract: Seven flavonoids were isolated from Eremosparton songoricum (Litv) Vass and their antioxidation actions were examined. To investigate protective ability against DNA damage induced by H2O2, human peripheral blood mononuclear cells (PBMCs) were incubated with 5-hydroxy-7,4′-dimethoxyflavone,5,7-dihydroxy-3′,4′-dimethoxyflavone, acacetin, chrysoeriol, pedalitin, flavogadorinin and 5,7,4′-trihydroxy-3′-methoxyflavone-4′-O-D-glucoside separately and then treated by H2O2. The percentage of damaged cells were determined by single-cell gel electrophoresis. To examine their inhibition of lipid peroxidation, the compounds were incubated with rat microsomes and the reaction was initiated by Fe2+/Vc. The lipid peroxidation product thiobarbituric acid reactive substances (TBARS) was determined. The result showed most of flavonoids had concentration-dependent antioxdative activities. The relationship of antioxidation and the structure is discussed.

Determination of acacetin in Xiangjuganmao Keli (no sweet) by HPLC

Abstract: OBJECTIVE To establish a method for the determination of acacetin in Xiangjuganmao Keli (no sweet). METHODS Acacetin in powdered herb was extracted by ultrasonator with methanol and was hydrolyzed with hydrochloric acid. Separation was accomplished on an ODS reversed phase column (5 microm, 4.6 x 250 mm) with a mobile phase of methanol-water-acetic acid(350: 150: 2). The detective wavelength was at 340 nm. RESULTS The method was accurate, the results were stable and reproducible. The linear range of calibration cure was within the concentration of 2.00 - 10.00 microg/ml (r = 0.9998). The average extraction recovery was 99.9% (n = 6), RSD = 0.41% (n = 6). CONCLUSION The method is simple, convenient, sensitive, and reproducible for quality control of Xiangjuganmao Keli (no sweet).

Studies on chemical constituents from Elsholtzia bodinieri Vaniot(I) in Gansu,China

Abstract: Aim To investigate the chemical constituents from the whole plant of Elsholtzia bodinieri in Gansu,China.Method The chemical constituents were extracted and separated by using silica gel column chromatography and TCL.The structures were elucidated by chemical and spectral analysis.Results Six compounds were isolated from the alcoholic extract of the whole plant of Elsholtzia bodinieri,their structures were elucidated as sandaracopimar-15-en-8β,12β-diol(1),epigallocatechin(2),tricin(3),acacetin(4),quercetin(5) and β-sitosterol(6).Conclusion Compound 1 and 2 are isolated from this species for the first time.