Acacetin

About: Acacetin is a research topic. Over the lifetime, 442 publications have been published within this topic receiving 10458 citations. The topic is also known as: 5,7-Dihydroxy-2-(4-methoxyphenyl)-4-benzopyrone & Linarigenin.

The Para-O-Methylation of Apigenin to Acacetin by Cell-Free Extracts of Robinia pseudoacacia L.

Abstract: Crude extracts from young Robinia pseudoacacia seedlings, shoots, and callus tissue catalyze the para-O-methylation of apigenin to acacetin using S-adenosyl-ʟ-methionine as methyl donor. Optimum activity was exhibited at pH 9.0, and Mg²⁺ was not required for maximum activity. EDTA (10 mᴍ) did not affect the reaction rate, but 47% inhibition was observed with SAH (100 μᴍ). β-Mercaptoethanol (5 mᴍ) was required in the homogenization medium for optimum O-methyltransferase activity. Apigenin (Kₘ, 50 μᴍ) was the best substrate, but significant activity was shown towards caffeic acid, 5-hydroxyferulic acid, naringenin, and quercetin. Paracoumaric, ferulic, and sinapic acids were not methylated. The Kₘ for S-adenosyl-ʟ-methionine was 31 μᴍ. Our demonstration of a para-O-methyltransferase activity methylating apigenin, but not para-coumaric acid, strongly supports the conclusion that the B-ring methylation pattern of acacetin is determined at the C₁₅-level in Robinia pseudoacacia.

Chemical Constituents of Pedicularis cephalantha Franch and P. siphonantha Don

Abstract: Thirteen compounds were isolated from the whole plants of Pedicularis cephalantha,which were identified as(±)-pinoresinol(1),acacetin(2),luteolin(3),7-deoxygardoside(4),yuheinoside(5),euphroside(6),mussaenosidic acid(7),mussaenoside(8),aucubin(9),martynoside(10),isomartynoside(11),clerodenoside A(12)and kidjolanin(13).Four compounds were isolated from the whole plants of P.siphonantha,which were identified as(+)-dehydrovomifoliol(14),vomifoliol(15),ω-hydroxypropioguaiacone(16)and 3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(17).

Acacetin Protects against Non-Alcoholic Fatty Liver Disease by Regulating Lipid Accumulation and Inflammation in Mice

Abstract: We previously demonstrated that acacetin reduces adipogenesis in adipocytes, and decreases lipid accumulation in visceral adipocyte tissue. Here we investigated whether acacetin regulated the mechanisms of lipogenesis and inflammation in non-alcoholic fatty liver disease (NAFLD) in obese mice. Male C57BL/6 mice were fed a high-fat diet (HFD), and then administered acacetin by intraperitoneal injection. Acacetin reduced body weight and liver weight in obese mice. Acacetin-treated obese mice exhibited decreased lipid accumulation, increased glycogen accumulation, and improved hepatocyte steatosis. Acacetin regulated triglycerides and total cholesterol in the liver and serum. Acacetin decreased low-density lipoprotein and leptin concentrations, but increased high-density lipoprotein and adiponectin levels in obese mice. Acacetin effectively weakened the gene expressions of transcription factors related to lipogenesis, and promoted the expressions of genes related to lipolysis and fatty acid β-oxidation in liver. Acacetin also reduced expressions of inflammation-related cytokines in the serum and liver. Oleic acid induced lipid accumulation in murine FL83B hepatocytes, and the effects of acacetin treatment indicated that acacetin may regulate lipid metabolism through the AMPK pathway. Acacetin may protect against hepatic steatosis by modulating inflammation and AMPK expression.

Bioactive Constituents of Ziziphora clinopodioides

Abstract: Ziziphora clinopodioides Lam. (Labiatae), a medicinal and edible plant, is mainly distributed in Xinjiang of China, Iran, Turkey, Mongolia, and Central Asia. It is commonly used in traditional Uyghur medicine for the treatment of fever, edema, neurasthenic, insomnia, tracheitis, lung abscess, hemorrhoids, hypertension, angina pectoris, coronary artery disease, and other cardiovascular diseases [1–4]. Phytochemical investigations on the genus Ziziphora have mainly focused on essential oil components as well as a few flavonoids, caffeoyl derivatives, fatty acids, phenolic acids, triterpenoids, and sterols [4]. In our previous studies, Z. clinopodioides Lam. was shown to acquire notable antihypertensive, antidiabetic, and antioxidant effects. In order to explore the antihypertensive constituents of this plant, the commonly adopted in vitro model of rat thoracic aortic rings [4] were applied for the bioassay-guided fractionation of active components from Z. clinopodioides Lam., with 11 compounds isolated from the 70% EtOH extract. Based on spectroscopic analyses, their structures were identified as diosmetin (1), apigenin (2), luteolin (3), caffeic acid (4), 5,7,2 -trihydroxyflavone 2 -O-D-glucopyranoside (5), methyl rosmarinate (6), betulinic acid (7), dibutyl phthalate (8), oleanolic acid (9), acacetin (10), and 5,6,4 -trihydroxy-7,8,3 -trimethoxyflavone (thymonin, 11), respectively, among which compounds 7 and 8 were obtained from the genus Ziziphora for the first time. Apigenin (2), luteolin (3), methyl rosmarinate (6), and oleanolic acid (9) were identified as potential vasorelaxant principles. Methyl rosmarinate (6), caffeic acid (4), and luteolin (3) possessed significant antioxidant capacities. Ziziphora clinopodioides along with the active principle oleanolic acid (9) were also reported to possess exciting antidiabetic properties herein for the first time.

Isolation and identification of chemical constituents from the whole plants of Ajuga decumbens

Abstract: Objective To isolate the constituents from the whole plants of Ajuga decumbens and elucidate their structures.Methods The compounds were isolated by silica gel column,ODS column,semi-preparative HPLC and preparative TLC chromatography and the 1H-NMR,13C-NMR spectroscopic analysis were employed for the structural elucidation.Results Fifteen compounds were isolated from the EtOAc extract of the whole plants of A.decumbens.They are ajugacumbin A(1),ajugacumbin B(2),ajuganipponin B(3),ajugamarin F4(4),ajugarin I(5),ajugamarin A1(6),ajugamarin A1 chlorhydrin(7),apigenin(8),luteolin(9),acacetin(10),methyl caffeate(11),4-hydroxy-4-(3-oxo-1-butenyl)-3,5,5-trimethylcyclohex-2-en-1-one(12),vanillic acid(13),loliolide(14)and(10E,15Z)-9,12,13-trihydroxyoctadeca-10,15-dienoic acid(15).Conclusions Compounds 11 and 12 are obtained from the genus Ajuga for the first time,compounds 7 and 15 are isolated from A.decumbens for the first time.