Showing papers on "Acyl-CoA published in 1980"
TL;DR: By the use of an excess of CoA and by the inclusion of a bicarbonate buffer for pH control, a method for the preparation of high specific activity [lJ4C]acyl CoA’s, both saturated and unsaturated, with reproducible yields is developed.
102 citations
TL;DR: The enzyme appears to be present only in brain; rat liver, kidney, and spleen have no similar activity, and while the enzyme actively synthesizes ceramide from lignoceric acid, it is not significantly active for palmitic acid.
31 citations
TL;DR: Nuclear magnetic resonance studies show that the polysaccharide, a random coil in its free form, undergoes a major conformational transition upon enclosing long-chain acyl-CoA.
Abstract: MMP, a linear alpha 1 leads to 4 linked polymer of 3-O-methylmannose, regulates the fatty acid synthetase from Mycobacterium smegmatis by forming stoichiometric complexes with the long-chain acyl-CoA synthetase products. In agreement with previous proposals [Bloch, K. (1977) in Advances in Enzymology and Related Areas of Molecular Biology, ed. Meister, A. (Wiley, New York), Vol. 45, pp. 1-84], nuclear magnetic resonance studies show that the polysaccharide, a random coil in its free form, undergoes a major conformational transition upon enclosing long-chain acyl-CoA. The polysaccharide, probably in helical conformation in the complexed form, interacts with both the paraffinic chain and the CoA moieties of the included fatty acyl thioester.
24 citations
01 Jan 1980
TL;DR: It is concluded that high rates of unsaturated fatty acid production may be more important during adaptation to cold temperatures than in fullycold-acclimated organisms, but that adjustments in the desaturation rates of specific fatty acids may contribute to the steady state fatty acid composition of cold-acClimated organisms.
Abstract: This report reviews the effects of temperature acclimation upon membrane fatty acid and phosphatide composition in poikilothermic organisms and the metabolic adaptations presumed to be responsible for the restructuring of membrane lipids. The regulation of membrane fatty acid composition is discussed in the context of (1) those metabolic processes which may directly influence the composition of the fatty-acyl coenzyme A pool (such as the capacity to produce unsaturated fatty acids) and (2) those processes which may result in the selective incorporation of specific fatty acids (acyl- and phosphotransferase reactions) from the acyl CoA pool into membrane lipids. It is concluded that high rates of unsaturated fatty acid production may be more important during adaptation to cold temperatures than in fully cold-acclimated organisms, but that adjustments in the desaturation rates of specific fatty acids may contribute to the steady state fatty acid composition of cold-acclimated organisms. Results of experiments with isolated hepatocytes prepared from thermally-acclimated trout demonstrate: (1) higher absolute rates of lipogenesis in cold-acclimated trout; (2) a higher level of unsaturated fatty acid production in warm-acclimated trout; and (3) adjustments in the ability of hepatocytes from cold-acclimated trout to metabolize linolenic acid at cold temperatures, involving compensations in both desaturase and acyltransferase activities. It is further concluded that temperature dependent substrate preferences of acyl- and phosphotransferase enzymes may also contribute to the restructuring of membrane lipids.
9 citations