Topic
Acyltransferase
About: Acyltransferase is a research topic. Over the lifetime, 2389 publications have been published within this topic receiving 88167 citations. The topic is also known as: acyltransferase.
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TL;DR: In this article, the 3' untranslated region (3'UTR) of TGFβ receptor type 1, TGFBR1 was identified as one of the targets of miRNAs.
Abstract: Nutrients are utilized and re-constructed by endodermal epithelial cells (EECs) in yolk sac membranes in avian species. Sterol-O acyltransferase 1 (SOAT1) is the key enzyme to convert cholesterol to cholesteryl ester for delivery to growing embryos. During development, absorption of yolk is matched with significant changes of SOAT1 mRNA and enzyme activity. miRNAs regulate angiogenesis and metabolism during mammalian development. However, the involvement of miRNAs in lipid utilization during avian embryogenesis remains ambiguous. Using a miRNA sequencing technique, we found several candidate miRNAs and confirmed expression patterns with real time PCR. They were selected for as candidates targeting the receptor (TGFβ receptor type 1, TGFBR1) that may regulate SOAT1. Similar to SOAT1 mRNA accumulation, the gga-miR-181a-5p expression was gradually elevated during development, but the concentration of gga-miR-429-3p was in the opposite direction. Transfection with gga-miR-181a-5p or gga-miR-429-3p inhibited TGFBR1 and SOAT1 in EECs. The 3’ untranslated region (3’UTR) of TGFBR1 was then confirmed to be one of the targets of gga-miR-181a-5p and gga-miR-429-3p. Taken together, expression of miRNAs during embryonic development regulates SOAT1 expression by inhibiting the 3’UTR of TGFBR1. This is indicative of possible regulation of avian yolk lipid utilization and modification of hatchability by changing miRNA expressions.
Patent•
12 Jun 2008
TL;DR: In this article, a novel glycerol-3-phosphate acyltransferase gene was described and a nucleic acid comprising a nucleotide sequence depicted in SEQ ID NO:1 or 4 was revealed.
Abstract: Disclosed is a novel glycerol-3-phosphate acyltransferase gene. Specifically disclosed is a nucleic acid comprising a nucleotide sequence depicted in SEQ ID NO:1 or 4 or a fragment of the nucleic acid.
01 Jan 1990
TL;DR: Themeadowfoam LPAacyltransferase is unique in itsability toproduce dierucoyl-PA, and should be a prime candidate for use in the production oftrierucin oils inrapeseed viagenetic engineering.
Abstract: Lysophosphatidate (LPA)acyltransferase (EC2.3.1.51) inthe microsomes fromthematuring seedsofmeadowfoam(Lim- nanthes alba), nasturtium (Tropaeolum majus), palm(Syagrus cocoides), castor bean(Ricinus communis), soybean (Glycine max),maize(Zeamays), andrapeseed (Brassica napus) were tested fortheir specificities toward1-oleoyl-LPA or1-erucoyl- LPA,andoleoyl coenzymeA (CoA)orerucoyl CoA.Allthe enzymescould useeither ofthetwoacyl acceptors andoleoyl CoA,butonlythemeadowfoam enzymecoulduseerucoyl CoA astheacyldonortoproduce dierucoyl phosphatidic acid(PA). Themeadowfoam enzymewasstudied further. Ithadanoptimal activity atpH7to8,anditsactivity wasinhibited by1millimolar MnCI2, ZnCI2, orp-chloromercuribenzoate. Inatestofsubstrate specificity using increasing concentrations ofeither 1-oleoyl-LPA or1-erucoyl-LPA, andeither oleoyl CoAorerucoyl CoA,the enzymeactivity inproducing PA washighest fordioleoyl-PA, followed successively by1-oleoyl-2-erucoyl-PA, dierucoyl-PA, and1-erucoyl-2-oleoyl-PA. Inatestofsubstrate selectivity using afixed combined concentration, butvarying proportions, of1- oleoyl-LPA and1-erucoyl-LPA, andofoleoyl CoAanderucoyl CoA,theenzymeshowedapattem ofacyl preference similar to that observed inthetestofsubstrate specificity, buttheprefer- encetoward oleoyl moiety inthesubstrates wasslightly stronger. Themeadowfoammicrosomes couldconvert(14C)glycerol-3- phosphate todiacylglycerols andtriacylglycerols inthepresence oferucoyl CoA.Themeadowfoam LPAacyltransferase isunique initsability toproduce dierucoyl-PA, andshouldbea prime candidate foruseintheproduction oftrierucin oils inrapeseed viagenetic engineering. Inoilseeds, TAG3issynthesized fromfatty acidviathe Kennedypathway whichconsists offourmajorenzymatic reactions (14). Glycerol-3-P isfirst acylated atthesn-1posi- tionbyglycerol-3-P AT,andtheproduct 1-LPAisacylated byLPA-ATtoformPA.PAisthendephosphorylated byPA
Patent•
01 Mar 1988
TL;DR: In this article, a lecithin-cholesterol acyltransferase was used to synthesize glycerol 3-phosphate, which was then used for assaying leCithin and free cholesterol.
Abstract: Process for assaying lecithin-cholesterol acyltransferase. The sample is placed in contact with a lecithin and free cholesterol as substrate, the lysolecithin produced is reacted with lysophospholipase and with glycerophosphocholine phosphodiesterase in order to produce glycerol 3-phosphate which is assayed.
Patent•
08 Apr 2009TL;DR: In this paper, a method for reducing the amount of cholesterol and improving the texture and reducing weight loss and/or increasing the fat stability of a meat based food product comprising: a) contacting meat with a lipid acyltransferase; b) incubating the meat contacted with the lipid acytransferase at a temperature between about 1° C. to about 70° C; c) producing a food product from the meat; wherein step b) is conducted before, during or after step c). Use of a lipid transferase to reduce cholesterol in a meat-based food product.
Abstract: A method for reducing the amount of cholesterol and/or improving the texture and/or reducing weight loss and/or increasing the fat stability of a meat based food product comprising: a) contacting meat with a lipid acyltransferase; b) incubating the meat contacted with the lipid acyltransferase at a temperature between about 1° C. to about 70° C.; c) producing a food product from the meat; wherein step b) is conducted before, during or after step c). Use of a lipid acyltransferase to reduce cholesterol in a meat based food product.