Showing papers on "Adaptive optics published in 2010"

Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues

Abstract: Biological specimens are rife with optical inhomogeneities that seriously degrade imaging performance under all but the most ideal conditions. Measuring and then correcting for these inhomogeneities is the province of adaptive optics. Here we introduce an approach to adaptive optics in microscopy wherein the rear pupil of an objective lens is segmented into subregions, and light is directed individually to each subregion to measure, by image shift, the deflection faced by each group of rays as they emerge from the objective and travel through the specimen toward the focus. Applying our method to two-photon microscopy, we could recover near-diffraction-limited performance from a variety of biological and nonbiological samples exhibiting aberrations large or small and smoothly varying or abruptly changing. In particular, results from fixed mouse cortical slices illustrate our ability to improve signal and resolution to depths of 400 microm.

In situ wavefront correction and its application to micromanipulation

Abstract: In any optical system, distortions to a propagating wavefront reduce the spatial coherence of a light field, making it increasingly difficult to obtain the theoretical diffraction-limited spot size. Such aberrations are severely detrimental to optimal performance in imaging, nanosurgery, nanofabrication and micromanipulation, as well as other techniques within modern microscopy. We present a generic method based on complex modulation for true in situ wavefront correction that allows compensation of all aberrations along the entire optical train. The power of the method is demonstrated for the field of micromanipulation, which is very sensitive to wavefront distortions. We present direct trapping with optimally focused laser light carrying power of a fraction of a milliwatt as well as the first trapping through highly turbid and diffusive media. This opens up new perspectives for optical micromanipulation in colloidal and biological physics and may be useful for various forms of advanced imaging.

The Design and Construction of Large Optical Telescopes

Abstract: Astronomical Observations / Instruments / Design Methods and Project Management / Telescope Optics / Stray Light Control / Telescope Structure and Mechanisms / Pointing and Control / Active and Adaptive Optics / Thermal Control / Integration and Verification / Observatory Enclosure / Observatory Sites /

First light AO (FLAO) system for LBT: final integration, acceptance test in Europe, and preliminary on-sky commissioning results

Abstract: In this paper we present the laboratory characterization and performance evaluation of the First Light Adaptive Optics (FLAO) the Natural Guide Star adaptive optics system for the Large Binocular Telescope (LBT). The system uses an adaptive secondary mirror with 672 actuators and a pyramid wavefront sensor with adjustable sampling of the telescope pupil from 30×30 down to 4×4 subapertures. The system was fully assembled in the Arcetri Observatory laboratories, passing the acceptance test in December 2009. The performance measured during the test were closed to goal specifications for all star magnitudes. In particular FLAO obtained 83% Strehl Ratio (SR) in the bright end (8.5 magnitudes star in R band) using H band filter and correcting 495 modes with 30×30 subapertures sampling. In the faint end (16.4 magnitude) a 5.0% SR correcting 36 modes with 7×7 subapertures was measured. The seeing conditions for these tests were 0.8" (r0 = 0.14m @ 550 nm) and an average wind speed of 15m/s. The results at other seeing conditions up to 1.5" are also presented. The system has been shipped to the LBT site, and the commissioning is taking place since March to December 2010. A few on sky results are presented.

Adaptive Optics Retinal Imaging: Emerging Clinical Applications

Abstract: The human retina is a uniquely accessible tissue. Tools like scanning laser ophthalmoscopy and spectral domain-optical coherence tomography provide clinicians with remarkably clear pictures of the living retina. Although the anterior optics of the eye permit such non-invasive visualization of the retina and associated pathology, the same optics induce significant aberrations that obviate cellular-resolution imaging in most cases. Adaptive optics (AO) imaging systems use active optical elements to compensate for aberrations in the optical path between the object and the camera. When applied to the human eye, AO allows direct visualization of individual rod and cone photoreceptor cells, retinal pigment epithelium cells, and white blood cells. AO imaging has changed the way vision scientists and ophthalmologists see the retina, helping to clarify our understanding of retinal structure, function, and the etiology of various retinal pathologies. Here, we review some of the advances that were made possible with AO imaging of the human retina and discuss applications and future prospects for clinical imaging.

Optical Communications for High-Altitude Platforms

Abstract: This paper contains a review of technologies, theoretical studies, and experimental field trials for optical communications from and to high-altitude platforms (HAPs). We discuss the pointing, acquisition, and tracking of laser terminals and describe how laser beams with low divergence can be used to transmit data at multi-Gigabits per second. Investigating the influence of the atmosphere, background light, and flight qualification requirements on system design, we explain why the data rates in free-space optical communications are still significantly below those possible in today's terrestrial fiber-based systems. Techniques like forward-error correction, adaptive optics, and diversity reception are discussed. Such measures help to increase the data rate or link distance while keeping the bit error ratio and outage probability of the optical HAP communication system low.

Commissioning status of Subaru laser guide star adaptive optics system

Abstract: The current status of commissioning and recent results in performance of Subaru laser guide star adaptive optics system is presented. After the first light using natural guide stars with limited configuration of the system in October 2006, we concentrated to complete a final configuration for a natural guide star to serve AO188 to an open use observation. On sky test with full configurations using natural guide star started in August 2008, and opened to a public one month later. We continuously achieved around 0.6 to 0.7 of Strehl ratio at K band using a bright guide star around 9th to 10th magnitude in R band. We found an unexpectedly large wavefront error in our laser launching telescope. The modification to fix this large wavefront error was made and we resumed the characterization of a laser guide star in February 2009. Finally we obtained a round-shaped laser guide star, whose image size is about 1.2 to 1.6 arcsec under the typical seeing condition. We are in the final phase of commissioning. A diffraction limited image by our AO system using a laser guide star will be obtained in the end of 2010. An open use observation with laser guide star system will start in the middle of 2011.

Performance of Subaru adaptive optics system AO188

Abstract: Subaru adaptive optics system (AO188) is an 188-elements curvature sensor adaptive optics system that is operated in both natural and laser guide star modes. AO188 was installed at Nasmyth platform of the Subaru telescope and it has been successfully operating in the natural guide star mode since October 2008. The performance of AO188 in the natural guide star mode has been well verified from on-sky data obtained with the infrared camera and spectrograph (IRCS). Under normal seeing condition, AO188 achieves K-band Strehl ratio between 60% and 70% using R =9 .0 magnitude natural guide stars and it works well with faint guide stars down to R =1 6.5 magnitude. We measured the FWHM and Strehl ratio of stellar images in globular clusters and found that the isoplanatic angle is approximately 30 arcsec. In this paper, we describe an overview of the operation procedure for AO188, as well as its performance such as angular resolution, Strehl ration, and sensitivity gain for detecting faint objects.

Gabor-based fusion technique for Optical Coherence Microscopy

Abstract: We recently reported on an Optical Coherence Microscopy technique, whose innovation intrinsically builds on a recently reported - 2 µm invariant lateral resolution by design throughout a 2 mm cubic full-field of view - liquid-lens-based dynamic focusing optical probe [Murali et al., Optics Letters 34, 145-147, 2009]. We shall report in this paper on the image acquisition enabled by this optical probe when combined with an automatic data fusion method developed and described here to produce an in-focus high resolution image throughout the imaging depth of the sample. An African frog tadpole (Xenopus laevis) was imaged with the novel probe and the Gabor-based fusion technique, demonstrating subcellular resolution in a 0.5 mm (lateral) x 0.5 mm (axial) without the need, for the first time, for x-y translation stages, depth scanning, high-cost adaptive optics, or manual intervention. In vivo images of human skin are also presented.

Kernel phase in fizeau interferometry

Abstract: The detection of high contrast companions at small angular separation appears feasible in conventional direct images using the self-calibration properties of interferometric observable quantities. The friendly notion of closure phase, which is key to the recent observational successes of non-redundant aperture masking interferometry used with adaptive optics, appears to be one example of a wide family of observable quantities that are not contaminated by phase noise. In the high-Strehl regime, soon to be available thanks to the coming generation of extreme adaptive optics systems on ground-based telescopes, and already available from space, closure phase like information can be extracted from any direct image, even taken with a redundant aperture. These new phase-noise immune observable quantities, called kernel phases, are determined a priori from the knowledge of the geometry of the pupil only. Re-analysis of archive data acquired with the Hubble Space Telescope NICMOS instrument using this new kernel-phase algorithm demonstrates the power of the method as it clearly detects and locates with milliarcsecond precision a known companion to a star at angular separation less than the diffraction limit.

Adaptive optics scanning laser ophthalmoscope with integrated wide-field retinal imaging and tracking

Abstract: We have developed a new, unified implementation of the adaptive optics scanning laser ophthalmoscope (AOSLO) incorporating a wide-field line-scanning ophthalmoscope (LSO) and a closed-loop optical retinal tracker. AOSLO raster scans are deflected by the integrated tracking mirrors so that direct AOSLO stabilization is automatic during tracking. The wide-field imager and large-spherical-mirror optical interface design, as well as a large-stroke deformable mirror (DM), enable the AOSLO image field to be corrected at any retinal coordinates of interest in a field of >25 deg. AO performance was assessed by imaging individuals with a range of refractive errors. In most subjects, image contrast was measurable at spatial frequencies close to the diffraction limit. Closed-loop optical (hardware) tracking performance was assessed by comparing sequential image series with and without stabilization. Though usually better than 10 μm rms, or 0.03 deg, tracking does not yet stabilize to single cone precision but significantly improves average image quality and increases the number of frames that can be successfully aligned by software-based post-processing methods. The new optical interface allows the high-resolution imaging field to be placed anywhere within the wide field without requiring the subject to re-fixate, enabling easier retinal navigation and faster, more efficient AOSLO montage capture and stitching.

Fast minimum variance wavefront reconstruction for extremely large telescopes

Abstract: We present what we believe to be a new algorithm, FRactal Iterative Method (FRiM), aiming at the reconstruction of the optical wavefront from measurements provided by a wavefront sensor. As our application is adaptive optics on extremely large telescopes, our algorithm was designed with speed and best quality in mind. The latter is achieved thanks to a regularization that enforces prior statistics. To solve the regularized problem, we use the conjugate gradient method, which takes advantage of the sparsity of the wavefront sensor model matrix and avoids the storage and inversion of a huge matrix. The prior covariance matrix is, however, non-sparse, and we derive a fractal approximation to the Karhunen–Loeve basis thanks to which the regularization by Kolmogorov statistics can be computed in O(N) operations, with N being the number of phase samples to estimate. Finally, we propose an effective preconditioning that also scales as O(N) and yields the solution in five to ten conjugate gradient iterations for any N. The resulting algorithm is therefore O(N). As an example, for a 128×128 Shack–Hartmann wavefront sensor, the FRiM appears to be more than 100 times faster than the classical vector-matrix multiplication method.

The adaptive secondary mirror for the Large Binocular Telescope: optical acceptance test and preliminary on-sky commissioning results

Abstract: The Large Binocular Telescope (LBT) has two adaptive secondary mirrors based on 672 voice-coil force actuators. The shape of the mirror is controlled using internal metrology based on co-located capacitive sensors. The first mirror unit is currently mounted on LBT for on-sky commissioning as part of the First Light Adaptive Optics System (FLAO). During spring-time 2009 the optical acceptance test was performed using the 14-m optical test tower at the Osservatorio Astrofisico di Arcetri (INAF) showing the capability of flattening the shell at the level of 14nm rms residual surface error. This paper reports the optical layout, calibration procedures and results of the optical acceptance test. Moreover we report the first results obtained during the early runs of FLAO commissioning showing the ability of the mirror to compensate for atmospheric turbulence with extremely high Strehl ratio values (better than 80% in H-band) as permitted by the largest number of correcting degrees of freedom currently available on-sky for astronomical telescopes.

Coherent optical link through the turbulent atmosphere

Abstract: We describe the realization of a 5 km free-space coherent optical link through the turbulent atmosphere between a telescope and a ground target. We present the phase noise of the link, limited mainly by atmospheric turbulence and mechanical vibrations of the telescope and the target. We discuss the implications of our results for applications, with particular emphasis on optical Doppler ranging to satellites and long-distance frequency transfer.

Tomography approach for multi-object adaptive optics

Abstract: Multi-object adaptive optics (MOAO) is a solution developed to perform a correction by adaptive optics (AO) in a science large field of view. As in many wide-field AO schemes, a tomographic reconstruction of the turbulence volume is required in order to compute the MOAO corrections to be applied in the dedicated directions of the observed very faint targets. The specificity of MOAO is the open-loop control of the deformable mirrors by a number of wavefront sensors (WFSs) that are coupled to bright guide stars in different directions. MOAO calls for new procedures both for the cross registration of all the channels and for the computation of the tomographic reconstructor. We propose a new approach, called "Learn and Apply (L&A)", that allows us to retrieve the tomographic reconstructor using the on-sky wavefront measurements from an MOAO instrument. This method is also used to calibrate the registrations between the off-axis wavefront sensors and the deformable mirrors placed in the science optical paths. We propose a procedure linking the WFSs in the different directions and measuring directly on-sky the required covariance matrices needed for the reconstructor. We present the theoretical expressions of the turbulence spatial covariance of wavefront slopes allowing one to derive any turbulent covariance matrix between two wavefront sensors. Finally, we discuss the convergence issue on the measured covariance matrices, we propose the fitting of the data based on the theoretical slope covariance using a reduced number of turbulence parameters, and we present the computation of a fully modeled reconstructor.

Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy

Abstract: The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration.

The Vector Vortex Coronagraph: sensitivity to central obscuration, low-order aberrations, chromaticism, and polarization

Abstract: The Vector Vortex Coronagraph is a phase-based coronagraph, one of the most efficient in terms of inner working angle, throughput, discovery space, contrast, and simplicity. Using liquid-crystal polymer technology, this new coronagraph has recently been the subject of lab demonstrations in the near-infrared, visible and was also used on sky at the Palomar observatory in the H and K bands (1.65 and 2.2 μm, respectively) to image the brown dwarf companion to HR 7672, and the three extra-solar planets around HR 8799. However, despite these recent successes, the Vector Vortex Coronagraph is, as are most coronagraphs, sensitive to the central obscuration and secondary support structures, low-order aberrations (tip-tilt, focus, etc), bandwidth (chromaticism), and polarization when image-plane wavefront sensing is performed. Here, we consider in detail these sensitivities as a function of the topological charge of the vortex and design features inherent to the manufacturing technology, and show that in practice all of them can be mitigated to meet specific needs.

Laboratory characterization and performance of the high-order adaptive optics system for the Large Binocular Telescope

Abstract: We present the laboratory characterization and performance evaluation of the First Light Adaptive Optics (FLAO), the natural guide star adaptive optics system for the Large Binocular Telescope (LBT). The system uses an adaptive secondary mirror with 672 actuators and a pyramid wavefront sensor with adjustable sampling of the telescope pupil from 30×30 down to 7×7 subapertures. The system was fully assembled in the Arcetri Observatory laboratories and passed its acceptance test in December 2009. The performance measured at different star magnitudes during the acceptance test is shown to lie between the baseline and the goal specifications. In particular, FLAO obtained a 77% Strehl ratio (SR) in the bright end (8.5 magnitude star in the R band) using an H-band filter and correcting 495 modes with 30×30 subaperture sampling. In the faint end (16.4 magnitude), a 5% SR correcting 36 modes with 7×7 subapertures was measured. The seeing conditions for these tests were 0.8 arcsec (r0=0.14 m at 550 nm) and an average wind speed of 15 m/s. The results for other seeing conditions up to 1.5 arcsec are also presented. The system has been shipped to the LBT site in Arizona, and its commissioning started in March 2010.

Wavefront aberration measurements and corrections through thick tissue using fluorescent microsphere reference beacons

Abstract: We present a new method to directly measure and correct the aberrations introduced when imaging through thick biological tissue. A Shack-Hartmann wavefront sensor is used to directly measure the wavefront error induced by a Drosophila embryo. The wavefront measurements are taken by seeding the embryo with fluorescent microspheres used as “artificial guide-stars.” The wavefront error is corrected in ten millisecond steps by applying the inverse to the wavefront error on a micro-electro-mechanical deformable mirror in the image path of the microscope. The results show that this new approach is capable of improving the Strehl ratio by 2 times on average and as high as 10 times when imaging through 100 μm of tissue. The results also show that the isoplanatic half-width is approximately 19 μm resulting in a corrected field of view 38 μm in diameter around the guide-star.

European Solar Telescope: Progress status

Abstract: In this paper, the present status of the development of the design of the European Solar Telescope is described The telescope is devised to have the best possible angular resolution and polarimetric performance, maximizing the throughput of the whole system To that aim, adaptive optics and multi-conjugate adaptive optics are integrated in the optical path The system will have the possibility to correct for the diurnal variation of the distance to the turbulence layers, by using several deformable mirrors, conjugated at different heights The present optical design of the telescope distributes the optical elements along the optical path in such a way that the instrumental polarization induced by the telescope is minimized and independent of the solar elevation and azimuth This property represents a large advantage for polarimetric measurements The ensemble of instruments that are planned is also presented

High resolution multimodal clinical ophthalmic imaging system

Abstract: We developed a multimodal adaptive optics (AO) retinal imager which is the first to combine high performance AO-corrected scanning laser ophthalmoscopy (SLO) and swept source Fourier domain optical coherence tomography (SSOCT) imaging modes in a single compact clinical prototype platform. Such systems are becoming ever more essential to vision research and are expected to prove their clinical value for diagnosis of retinal diseases, including glaucoma, diabetic retinopathy (DR), age-related macular degeneration (AMD), and retinitis pigmentosa. The SSOCT channel operates at a wavelength of 1 µm for increased penetration and visualization of the choriocapillaris and choroid, sites of major disease activity for DR and wet AMD. This AO system is designed for use in clinical populations; a dual deformable mirror (DM) configuration allows simultaneous low- and high-order aberration correction over a large range of refractions and ocular media quality. The system also includes a wide field (33 deg.) line scanning ophthalmoscope (LSO) for initial screening, target identification, and global orientation, an integrated retinal tracker (RT) to stabilize the SLO, OCT, and LSO imaging fields in the presence of lateral eye motion, and a high-resolution LCD-based fixation target for presentation of visual cues. The system was tested in human subjects without retinal disease for performance optimization and validation. We were able to resolve and quantify cone photoreceptors across the macula to within ~0.5 deg (~100-150 µm) of the fovea, image and delineate ten retinal layers, and penetrate to resolve features deep into the choroid. The prototype presented here is the first of a new class of powerful flexible imaging platforms that will provide clinicians and researchers with high-resolution, high performance adaptive optics imaging to help guide therapies, develop new drugs, and improve patient outcomes.

Kernel-Phase in Fizeau Inteferometry

Abstract: The detection of high contrast companions at small angular separation appears feasible in conventional direct images using the self-calibration properties of interferometric observable quantities. The friendly notion of closure-phase, which is key to the recent observational successes of non-redundant aperture masking interferometry used with Adaptive Optics, appears to be one example of a wide family of observable quantities that are not contaminated by phase-noise. In the high-Strehl regime, soon to be available thanks to the coming generation of extreme Adaptive Optics systems on ground based telescopes, and already available from space, closure-phase like information can be extracted from any direct image, even taken with a redundant aperture. These new phase-noise immune observable quantities, called kernel-phases, are determined a-priori from the knowledge of the geometry of the pupil only. Re-analysis of archive data acquired with the Hubble Space Telescope NICMOS instrument, using this new kernel-phase algorithm demonstrates the power of the method as it clearly detects and locates with milli-arcsecond precision a known companion to a star at angular separation less than the diffraction limit.

An SLM-based Shack-Hartmann wavefront sensor for aberration correction in optical tweezers

Abstract: Holographic optical tweezers allow the creation of multiple optical traps in 3D configurations through the use of dynamic diffractive optical elements called spatial light modulators (SLMs). We show that, in addition to controlling traps, the SLM in a holographic tweezers system can be both the principal element of a wavefront sensor and the corrective element in a closed-loop adaptive optics system. This means that aberrations in such systems can be estimated and corrected without altering the experimental setup. Aberrations are estimated using the Shack–Hartmann method, where an array of spots is projected into the sample plane and the distortion of this array is used to recover the aberration. The system can recover aberrations of up to ten wavelengths peak–peak, and is sensitive to aberrations much smaller than a wavelength. The spot pattern could also be analysed by eye, as a tool for aligning the system.

GRAVITY: a four telescope beam combiner instrument for the VLTI

Abstract: GRAVITY is an adaptive optics assisted Beam Combiner for the second generation VLTI instrumentation. The instrument will provide high-precision narrow-angle astrometry and phase-referenced interferometric imaging in the astronomical K-band for faint objects. We describe the wide range of science that will be tackled with this instrument, highlighting the unique capabilities of the VLTI in combination with GRAVITY. The most prominent goal is to observe highly relativistic motions of matter close to the event horizon of Sgr A*, the massive black hole at center of the Milky Way. We present the preliminary design that fulfils the requirements that follow from the key science drivers: It includes an integrated optics, 4-telescope, dual feed beam combiner operated in a cryogenic vessel; near-infrared wavefrontsensing adaptive optics; fringe-tracking on secondary sources within the field of view of the VLTI and a novel metrology concept. Simulations show that 10 μas astrometry within few minutes is feasible for a source with a magnitude of mK = 15 like Sgr A*, given the availability of suitable phase reference sources (mK = 10). Using the same setup, imaging of mK = 18 stellar sources in the interferometric field of view is possible, assuming a full night of observations and the corresponding UV coverage of the VLTI.

Performance characterization of the HiCIAO instrument for the Subaru Telescope

Abstract: HiCIAO is a near-infrared, high contrast instrument which is specifically designed for searches and studies for extrasolar planets and proto-planetary/debris disks on the Subaru 8.2 m telescope. A coronagraph technique and three differential observing modes, i.e., a dual-beam simultaneous polarimetric differential imaging mode, quad-beam simultaneous spectral differential imaging mode, and angular differential imaging mode, are used to extract faint objects from the sea of speckle around bright stars. We describe the instrument performances verified in the laboratory and during the commissioning period. Readout noise with a correlated double sampling method is 15 e- using the Sidecar ASIC controller with the HAWAII-2RG detector array, and it is as low as 5 e- with a multiple sampling method. Strehl ratio obtained by HiCIAO on the sky combined with the 188-actuator adaptive optics system (AO188) is 0.4 and 0.7 in the H and K-band, respectively, with natural guide stars that have R ~ 5 and under median seeing conditions. Image distortion is correctable to 7 milli-arcsec level using the ACS data as a reference image. Examples of contrast performances in the observing modes are presented from data obtained during the commissioning period. An observation for HR 8799 in the angular differential imaging mode shows a clear detection of three known planets, demonstrating the high contrast capability of AO188+HiCIAO.

Experimental detection of optical vortices with a Shack-Hartmann wavefront sensor

Abstract: Laboratory experiments are carried out to detect optical vortices in conditions typical of those experienced when a laser beam is propagated through the atmosphere. A Spatial Light Modulator (SLM) is used to mimic atmospheric turbulence and a Shack-Hartmann wavefront sensor is utilised to measure the slopes of the wavefront surface. A matched filter algorithm determines the positions of the Shack-Hartmann spot centroids more robustly than a centroiding algorithm. The slope discrepancy is then obtained by taking the slopes measured by the wavefront sensor away from the slopes calculated from a least squares reconstruction of the phase. The slope discrepancy field is used as an input to the branch point potential method to find if a vortex is present, and if so to give its position and sign. The use of the slope discrepancy technique greatly improves the detection rate of the branch point potential method. This work shows the first time the branch point potential method has been used to detect optical vortices in an experimental setup.

High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing.

Abstract: Live imaging in cell biology requires three-dimensional data acquisition with the best resolution and signal-to-noise ratio possible. Depth aberrations are a major source of image degradation in three-dimensional microscopy, causing a significant loss of resolution and intensity deep into the sample. These aberrations occur because of the mismatch between the sample refractive index and the immersion medium index. We have built a wide-field fluorescence microscope that incorporates a large-throw deformable mirror to simultaneously focus and correct for depth aberration in three-dimensional imaging. Imaging fluorescent beads in water and glycerol with an oil immersion lens we demonstrate a corrected point spread function and a 2-fold improvement in signal intensity. We apply this new microscope to imaging biological samples, and show sharper images and improved deconvolution.

Hybrid adaptive-optics visual simulator

Abstract: We have developed a hybrid adaptive-optics visual simulator (HAOVS), combining two different phase-manipulation technologies: an optically addressed liquid-crystal phase modulator, relatively slow but capable of producing abrupt or discontinuous phase profiles; and a membrane deformable mirror, restricted to smooth profiles but with a temporal response allowing compensation of the eye's aberration fluctuations. As proof of concept, a phase element structured as discontinuous radial sectors was objectively tested as a function of defocus, and a correction loop was closed in a real eye. To further illustrate the capabilities of the device for visual simulation, we recorded extended images of different stimuli through the system by means of an external camera replacing the subject's eye. The HAOVS is specially intended as a tool for developing new ophthalmic optics elements, where it opens the possibility to explore designs with irregularities and/or discontinuities.

Self-coherent camera as a focal plane wavefront sensor: simulations

Abstract: Context. Direct detection of exoplanets requires high dynamic range imaging. Coronagraphs could be the solution, but their performance in space is limited by wavefront errors (manufacturing errors on optics, temperature variations, etc.), which create quasi-static stellar speckles in the final image. Aims. Several solutions have been suggested for tackling this speckle noise. Differential imaging techniques substract a reference image to the coronagraphic residue in a post-processing imaging. Other techniques attempt to actively correct wavefront errors using a deformable mirror. In that case, wavefront aberrations have to be measured in the science image to extremely high accuracy. Methods. We propose the self-coherent camera sequentially used as a focal-plane wavefront sensor for active correction and differential imaging. For both uses, stellar speckles are spatially encoded in the science image so that differential aberrations are strongly minimized. The encoding is based on the principle of light incoherence between the hosting star and its environment. Results. In this paper, we first discuss one intrinsic limitation of deformable mirrors. Then, several parameters of the self-coherent camera are studied in detail. We also propose an easy and robust design to associate the self-coherent camera with a coronagraph that uses a Lyot stop. Finally, we discuss the case of the association with a four-quadrant phase mask and numerically demonstrate that such a device enables detection of Earth-like planets under realistic conditions. Conclusions. The parametric study of the technique lets us believe it can be implemented quite easily in future instruments dedicated to direct imaging of exoplanets.

High-precision astrometry with MICADO at the European Extremely Large Telescope

Abstract: In this article, we identify and discuss various statistical and systematic effects influencing the astrometric accuracy achievable with Multi-adaptive optics Imaging CAmera for Deep Observations, the near-infrared (NIR) imaging camera proposed for the 42-m European Extremely Large Telescope. These effects are instrumental (e.g. geometric distortion), atmospheric (e.g. chromatic differential refraction) and astronomical (reference source selection). We find that there are several phenomena having impact on ∼100 μas scales, meaning they can be substantially larger than the theoretical statistical astrometric accuracy of an optical/NIR 42-m telescope. Depending on type, these effects need to be controlled via dedicated instrumental design properties or via dedicated calibration procedures. We conclude that if this is done properly, astrometric accuracies of 40 μas or better - with 40 μas yr ―1 in proper motions corresponding to ≈20 km s ―1 at 100 kpc distance - can be achieved in one epoch of actual observations.