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Agar plate

About: Agar plate is a(n) research topic. Over the lifetime, 4419 publication(s) have been published within this topic receiving 95488 citation(s).


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Journal ArticleDOI
TL;DR: The universal method to detect and determine siderophores was developed by using their high affinity for iron(III) and was successfully used to screen mutants in the iron uptake system of two Rhizobium meliloti strains, DM5 and 1021.
Abstract: A universal method to detect and determine siderophores was developed by using their high affinity for iron(III). The ternary complex chrome azurol S/iron(III)/hexadecyltrimethylammonium bromide, with an extinction coefficient of approximately 100,000 M-1 cm-1 at 630 nm, serves as an indicator. When a strong chelator removes the iron from the dye, its color turns from blue to orange. Because of the high sensitivity, determination of siderophores in solution and their characterization by paper electrophoresis chromatography can be performed directly on supernatants of culture fluids. The method is also applicable to agar plates. Orange halos around the colonies on blue agar are indicative of siderophore excretion. It was demonstrated with Escherichia coli strains that biosynthetic, transport, and regulatory mutations in the enterobactin system are clearly distinguishable. The method was successfully used to screen mutants in the iron uptake system of two Rhizobium meliloti strains, DM5 and 1021.

4,833 citations

Journal ArticleDOI
TL;DR: The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated.
Abstract: The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated. MIC values are used to determine susceptibilities of bacteria to drugs and also to evaluate the activity of new antimicrobial agents. Agar dilution involves the incorporation of different concentrations of the antimicrobial substance into a nutrient agar medium followed by the application of a standardized number of cells to the surface of the agar plate. For broth dilution, often determined in 96-well microtiter plate format, bacteria are inoculated into a liquid growth medium in the presence of different concentrations of an antimicrobial agent. Growth is assessed after incubation for a defined period of time (16-20 h) and the MIC value is read. This protocol applies only to aerobic bacteria and can be completed in 3 d.

3,266 citations

Journal ArticleDOI
TL;DR: The results indicated that the criterion for isolation of phosphate solubilizers based on the formation of visible halo/zone on agar plates is not a reliable technique, and soil microbes should be screened in NBRIP broth assay for the identification of the most efficient phosphate soluble inorganic phosphates in liquid medium.
Abstract: A novel defined microbiological growth medium, National Botanical Research Institute's phosphate growth medium (NBRIP), which is more efficient than Pikovskaya medium (PVK), was developed for screening phosphate solubilizing microorganisms. In plate assay the efficiency of NBRIP was comparable to PVK; however, in broth assay NBRIP consistently demonstrated about 3-fold higher efficiency compared to PVK. The results indicated that the criterion for isolation of phosphate solubilizers based on the formation of visible halo/zone on agar plates is not a reliable technique, as many isolates which did not show any clear zone on agar plates solubilized insoluble inorganic phosphates in liquid medium. It may be concluded that soil microbes should be screened in NBRIP broth assay for the identification of the most efficient phosphate solubilizers.

1,510 citations

01 Jan 2001
TL;DR: This work proposes that the Cdc48/p97–Ufd1–Npl4 complex extracts proteins from the ER membrane for cytosolic degradation, and demonstrates that it requires the interacting partners Ufd1 and Npl4.
Abstract: 6colony-forming units per 10 ml -1 in SFM supplemented with 0.3 mM calcium chloride, and inoculated onto the surface of the tissue. After inoculation, we incubated tissue samples at 37 8C with 5% CO2 and no supplemental humidity. Transwells containing the inoculated tissue samples were transferred to fresh blood agar every 2 h. The blood agar plates were then incubated overnight at 37 8C for enumeration of colony-forming units representing the number of organisms emerging from the basal surface of the tissue.

960 citations

Journal ArticleDOI
TL;DR: With the TSBV medium, even modestly equipped laboratories will be able to isolate and identify A. actinomycetemcomitans from clinical specimens, and this bacterium from Haemophilus aphrophilus, Capnocytophaga species, and a few other contaminating organisms.
Abstract: A selective medium, TSBV (tryptic soy-serum-bacitracin-vancomycin) agar, was developed for the isolation of Actinobacillus actinomycetemcomitans, TSBV agar contained (per liter) 40 g of tryptic soy agar, 1 g of yeast extract, 100 ml of horse serum 75 mg of bacitracin, and 5 mg of vancomycin The TSBV medium suppressed most oral species and permitted significantly higher recovery of A actinomycetemcomitans than nonselective blood agar medium The distinct colonial morphology and positive catalase reaction of A actinomycetemcomitans easily distinguished this bacterium from Haemophilus aphrophilus, Capnocytophaga species, and a few other contaminating organisms With the TSBV medium, even modestly equipped laboratories will be able to isolate and identify A actinomycetemcomitans from clinical specimens

754 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20222
202186
202087
201991
201894
2017117