Topic
Aldose
About: Aldose is a research topic. Over the lifetime, 1270 publications have been published within this topic receiving 27197 citations. The topic is also known as: aldoses.
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TL;DR: It is proposed that ALDRXV4 gene from X. viscosa could be a potential candidate for developing stress-tolerant crop plants and three-dimensional structure prediction based on homology modeling suggested several similarities with the other aldose reductases reported from plants.
Abstract: Aldose reductases are key enzymes in the detoxification of reactive aldehyde compounds like methylglyoxal (MG) and malondialdehyde. The present study describes for first time the preliminary biochemical and structural characterization of the aldose reductase (ALDRXV4) enzyme from the resurrection plant Xerophyta viscosa. The ALDRXV4 cDNA was expressed in E. coli using pET28a expression vector, and the protein was purified using affinity chromatography. The recombinant protein showed a molecular mass of ~36 kDa. The K
M (1.2 mM) and k
cat (14.5 s−1) of the protein determined using MG as substrate was found to be comparable with other reported homologs. Three-dimensional structure prediction based on homology modeling suggested several similarities with the other aldose reductases reported from plants. Circular dichroism spectroscopy results supported the bioinformatic prediction of alpha–beta helix nature of aldose reductase proteins. Subcellular localization studies revealed that the ALDRXV4-GFP fusion protein was localized both in the nucleus and the cytoplasm. The E. coli cells overexpressing ALDRXV4 exhibited improved growth and showed tolerance against diverse abiotic stresses induced by high salt (500 mM NaCl), osmoticum (10 % PEG 6000), heavy metal (20 mM CdCl2), and MG (5 mM). Based on these results, we propose that ALDRXV4 gene from X. viscosa could be a potential candidate for developing stress-tolerant crop plants.
3 citations
Journal Article•
3 citations
3 citations
Patent•
03 Mar 2000
TL;DR: In this article, a method for producing a compound represented by the formula (I) [R2 is substituted or non-substituted phenyl; R7 is H or OH; (n) is an integer of 1 to 4] is characterized by selectively binding a sugar derivative to a catechin compound through an O-glycoside, and then oxidizing the 4position of the flavonoid skeleton.
Abstract: PROBLEM TO BE SOLVED: To provide a method of simply producing a flavonoid compound which exhibits an aldose reductase-inhibiting action, an active oxygen-eliminating action, a carcinogenesis promotion-inhibiting action, or an antiinflammatory action, and is pharmacologically useful. SOLUTION: This method for producing the compound represented by the formula (I) [R2 is substituted or non-substituted phenyl; R7 is H or OH; (n) is an integer of 1 to 4], is characterized by selectively binding a sugar derivative to a catechin compound through an O-glycoside, and then oxidizing the 4-position of the flavonoid skeleton. COPYRIGHT: (C)2001,JPO
3 citations
3 citations