Topic
Alkaline phosphatase
About: Alkaline phosphatase is a research topic. Over the lifetime, 20218 publications have been published within this topic receiving 540547 citations. The topic is also known as: Alkaline_phosphatase & IPR001952.
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TL;DR: It is demonstrated that IL-6 inhibits bone nodule formation by rat calvaria cells in vitro and suggested that IL -6 may inhibit osteoblast differentiation, and the role in bone metabolism is uncertain.
126 citations
TL;DR: The results suggest that the induction of acid and ALP under salt stress may be due to a phosphorous deficiency in mangrove Bruguiera parviflora.
126 citations
TL;DR: Targeting of secreted proteins to T. gondii dense granules and the plasma membrane uses general mechanisms identified in higher eukaryotic cells but is simplified and exaggerated in scope, while targeting ofsecreted proteins beyond the boundaries of the parasite involves unusual sorting events.
Abstract: All known proteins that accumulate in the vacuolar space surrounding the obligate intracellular protozoan parasite Toxoplasma gondii are derived from parasite dense granules. To determine if constitutive secretory vesicles could also mediate delivery to the vacuolar space, T. gondii was stably transfected with soluble Escherichia coli alkaline phosphatase and E. coli β-lactamase. Surprisingly, both foreign secretory reporters were delivered quantitatively into parasite dense granules and efficiently secreted into the vacuolar space. Addition of a glycosylphosphatidylinositol membrane anchor rerouted alkaline phosphatase to the parasite surface. Alkaline phosphatase fused to the transmembrane domain and cytoplasmic tail from the endogenous dense granule protein GRA4 localized to dense granules. The protein was secreted into a tuboreticular network in the vacuolar space, in a fashion dependent upon the cytoplasmic tail, but not upon a tyrosine-based motif within the tail. Alkaline phosphatase fused to the vesicular stomatitis virus G protein transmembrane domain and cytoplasmic tail localized primarily to the Golgi, although staining of dense granules and the intravacuolar network was also detected; truncating the cytoplasmic tail decreased Golgi staining and increased delivery to dense granules but blocked delivery to the intravacuolar network. Targeting of secreted proteins to T. gondii dense granules and the plasma membrane uses general mechanisms identified in higher eukaryotic cells but is simplified and exaggerated in scope, while targeting of secreted proteins beyond the boundaries of the parasite involves unusual sorting events.
126 citations
TL;DR: Enzymes in the liver, followed by those in the kidneys and gills seem to be most seriously affected by Cd poisoning in this fish.
126 citations
TL;DR: Assays for acid and alkaline phosphatase levels in gingival fluid have been evaluated for their use as possible indicators of periodontal disease activity and presented as models of how substances can enter theperiodontal pocket by different routes.
Abstract: Microtiter assays for acid and alkaline phosphatase levels in gingival fluid (GF) have been evaluated for their use as possible indicators of periodontal disease activity. Alkaline phosphatase concentration was shown to be positively associated with periodontal disease activity, while acid phosphatase showed no relation to disease activity. When a series of timed gingival fluid samples was taken from several sites in one subject's mouth, reproducible differences in volume, alkaline phosphatase concentration, and total acid phosphatase were found between the first and subsequent samples. Acid and alkaline phosphatase are presented as models of how substances can enter the periodontal pocket by different routes.
125 citations