Topic
Alkaline phosphatase
About: Alkaline phosphatase is a research topic. Over the lifetime, 20218 publications have been published within this topic receiving 540547 citations. The topic is also known as: Alkaline_phosphatase & IPR001952.
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TL;DR: The results suggest that in vitro conditions may critically influence cell differentiation and protein expression in rabbit tendon defects and add osteogenic inducing supplements to the culture medium increases cellular protein in monolayer culture.
226 citations
TL;DR: In this paper, a batch reaction of disodium p-nitrophenyl phosphate with a 0.1 vol. % water solution in supercritical CO2 at 100 atm and 35°C was shown to produce disodium nitrophenol when catalyzed by alkaline phosphatase.
Abstract: The enzyme alkaline phosphatase, EC 3. 1. 3. 1, was found to be active in a supercritical carbo dioxide solvent system. A batch reaction of disodium p-nitrophenyl phosphate with a 0.1 vol. % water solution in supercritical CO2 at 100 atm and 35°C produced p-nitrophenol when catalyzed by alkaline phosphatase.
225 citations
TL;DR: An alkaline phosphatase isoen enzyme (Regan isoenzyme) has been identified in the serum of 27 patients with various malignant tumors and has been clinically useful in monitoring progression or regression of tumor, identifying a source of serum alkaline phosphate elevation, and identifying malignant effusions.
Abstract: An alkaline phosphatase isoenzyme (Regan isoenzyme) has been identified in the serum of 27 patients with various malignant tumors. This isoenzyme is biochemically and immunologically indistinguishable from placental alkaline phosphatase. In addition to being present in serum, it has been identified in both tumor tissue and malignant effusion fluids. Measurement of this isoenzyme has been clinically useful in monitoring progression or regression of tumor, identifying a source of serum alkaline phosphatase elevation, and identifying malignant effusions.
225 citations
TL;DR: The role of the small intestinal brush-border enzyme, intestinal alkaline phosphatase (IAP), in preventing a high-fat-diet–induced metabolic syndrome in mice is examined and it is found that both endogenous and orally supplemented IAP inhibits absorption of endotoxin (lipopolysaccharides) that occurs with dietary fat.
Abstract: Metabolic syndrome comprises a cluster of related disorders that includes obesity, glucose intolerance, insulin resistance, dyslipidemia, and fatty liver. Recently, gut-derived chronic endotoxemia has been identified as a primary mediator for triggering the low-grade inflammation responsible for the development of metabolic syndrome. In the present study we examined the role of the small intestinal brush-border enzyme, intestinal alkaline phosphatase (IAP), in preventing a high-fat-diet–induced metabolic syndrome in mice. We found that both endogenous and orally supplemented IAP inhibits absorption of endotoxin (lipopolysaccharides) that occurs with dietary fat, and oral IAP supplementation prevents as well as reverses metabolic syndrome. Furthermore, IAP supplementation improves the lipid profile in mice fed a standard, low-fat chow diet. These results point to a potentially unique therapy against metabolic syndrome in at-risk humans.
225 citations
TL;DR: The results support the possible development of in vitro clonal methods for the study of differentiation and regulation of the osteogenic and other fibroblastic cell lines of the marrow stromal system.
Abstract: Fibroblastic colonies, each of which is derived from a single precursor cell (CFU-F), are formed when suspensions of marrow cells are cultured in vitro. The ability of marrow CFU-F to differentiate in vitro was investigated using the expression of alkaline phosphatase activity as a marker for osteogenic differentiation. In cultures of rabbit marrow cells the colonies formed varied in size, morphology and expression of enzyme activity, indicating that marrow stromal CFU-F are a heterogeneous population. Growth and differentiation of marrow CFU-F can be modified in vitro. Epidermal growth factor increased average colony size and reduced clonal expression of alkaline phosphatase activity to very low levels. Hydrocortisone activated the osteogenic differentiation programme within the cellular progeny of a wide spectrum of CFU-F. The results support the possible development of in vitro clonal methods for the study of differentiation and regulation of the osteogenic and other fibroblastic cell lines of the marrow stromal system.
224 citations