Topic
Alkaline phosphatase
About: Alkaline phosphatase is a research topic. Over the lifetime, 20218 publications have been published within this topic receiving 540547 citations. The topic is also known as: Alkaline_phosphatase & IPR001952.
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TL;DR: The structure of alkaline phosphatase from Escherichia coli has been determined to 2.8 A resolution and accessibility calculations based on the refined co-ordinates of the enzyme show that the active pocket barely accommodates inorganic phosphate.
215 citations
TL;DR: In this paper, the authors evaluated the effect of trace elements on acid and alkaline phosphatase activity in soil and found that all trace elements inhibit the activity of phosphatases in soils.
Abstract: Studies to evaluate the effects on the activity of acid and alkaline phosphatases of 20 trace elements showed that all trace elements inhibit the activity of phosphatases in soils. Results showed that the relative effectiveness of the trace elements in inhibition of phosphatase activity depends on the soil. When the trace elements were compared by using 25 ..mu..mole/g soil, the average inhibition of acid phosphatase in three soils showed that Hg(II), As(V), W(VI), and Mo(VI) were the most effective (average inhibition >50%) and that Ba(II), Co(II), and As(III) were the least effective (average inhibition <10%) inhibitors. Other elements that inhibited acid phosphatase activity in soils were: Cu(I), Ag(I), Cd(II), Cu(II), Zn(II), Mn(II), Sn(II), Ni(II), Pb(II), Fe(II), Cr(III), Fe(III), B(III), Al(III), V(IV), and Se(IV); their degrees of effectiveness varied in the three soils used. The most effective inhibitors of alkaline phosphatase activity in soils were Ag(I), Cd(II), V(IV), and As(V). All the trace elements that inhibited acid phosphatase activity also inhibited alkaline phosphatase activity. The order of effectiveness of the trace elements in inhibition of acid phosphatase activity was different from that of inhibition of alkaline phosphatase activity. Generally, the inhibitory effect of trace elements was less in the presence ofmore » 2.5 ..mu..mole/g soil than in the presence of 25 ..mu..mole/g soil. In addition to the effect of trace elements, phosphate ion inhibited acid and alkaline phosphatase activities in soils. Related anions such as NO/sub 2//sup -/, NO/sub 3//sup -/, Cl/sup -/, and SO/sup 2 -//sub 4/ were not inhibitory in the presence and absence of buffer.« less
215 citations
TL;DR: The results suggest that sleep deprivation may decrease cell‐mediated immune reactions and thereby impair some aspects of host defense.
Abstract: The possible influence of 48 hr of sleep deprivation on in vitro DNA synthesis of blood lymphocytes and on the adhesiveness and intracellular, stainable activity of alkaline phosphatase in blood granulocytes was studied in twelve young male volunteers. Following the sleep deprivation, all 12 subjects showed marked reductions of DNA synthesis after stimulation with phytohemagglutinin. Pre-exposure levels were regained 5 days after terminating the vigil. No changes were noted in granulocyte adherence or alkaline phosphatase activity. The results suggest that sleep deprivation may decrease cell-mediated immune reactions and thereby impair some aspects of host defense.
214 citations
TL;DR: This work proposes an alternative nomenclature for the ALP isoenzymes and isoforms based on their structural characteristics: soluble, dimeric (Sol), anchor-bearing (Anch), and membrane-bound (Mem) liver, bone, intestinal, and placental A...
Abstract: Alkaline phosphatase (ALP, EC 3.1.3.1.) is a membrane-bound metalloenzyme that consists of a group of true isoenzymes, all glycoproteins, encoded for by at least four different gene loci: tissue-no...
213 citations
TL;DR: Findings indicate that the calcium phosphate-rich layer promotes adsorption and concentration of proteins from the TCM, and it is utilized by the osteoblasts to form the mineralized extracellular matrix.
213 citations