Alkaline phosphatase

About: Alkaline phosphatase is a research topic. Over the lifetime, 20218 publications have been published within this topic receiving 540547 citations. The topic is also known as: Alkaline_phosphatase & IPR001952.

Effect of Garlic Oil on the Levels of Various Enzymes in the Serum and Tissue of Streptozotocin Diabetic Rats

Abstract: Levels of red cell, serum acid, and alkaline phosphatases, serum amylase, alanine and aspartate transferase and bilirubin were examined in streptozotocin-induced diabetic rats treated with garlic oil and compared with the corresponding levels in diabetic control rats, normal rats and normal rats on garlic oil. Values of tissue amylase and total protein were also assessed from the pancreas, liver, and kidney. Treatment of diabetic rats with garlic oil significantly decreased the red cell phosphatase (p < 0.01), serum acid and alkaline phosphatase (p<0.001) when compared to diabetic control rats. Serum alanine and asparate transferases were significantly (p<0.001) decreased as well as serum amylase (p<0.002) in garlic oil treated diabetic rats as compared with diabetic control rats. When treated with garlic oil, however, diabetic and normal rats showed significant increase (p <0.05) in the amylase levels of the pancrease, liver, and kidney.

Studies on Zinc Deficiency: Changes in Trace Elements and Enzyme Activities in Tissues of Zinc-deficient Rats *

Abstract: Zinc content of testes, bones, esophagus, kidneys, and muscles was decreased, whereas iron content was increased in the testes of zinc-deficient rats compared to restrictedly fed control rats. Histochemical enzyme determinations revealed reduced activities of certain enzymes in the testes, bones, esophagus, and kidneys. In the testes, lactic dehydrogenase (LDH), malic dehydrogenase (MDH), alcohol dehydrogenase (ADH), and NADH diaphorase; in the bones, LDH, MDH, ADH, and alkaline phosphatase; in the esophagus, MDH, ADH, and NADH diaphorase; and in the kidneys, MDH and alkaline phosphatase were decreased in zinc-deficient rats compared to restrictedly fed controls. Succinic dehydrogenase (SDH) revealed no significant changes under the conditions of our experiments in various groups of rats that were investigated. In a "repleted" group of rats, content of zinc in testes and bones increased significantly, compared to the deficient group. The iron content of the testes decreased after repletion with zinc. In the testes, bones, esophagus, and kidneys, the activities of various enzymes increased after repletion with zinc. Inasmuch as the major manifestations of zinc deficiency syndrome in the rat include growth retardation, testicular atrophy, and esophageal parakeratosis, our results suggest that the content of zinc in the above tissues most likely controls the physiological processes through the formation of zinc-dependent enzymes.

New observations on the Regan isoenzyme of alkaline phosphatase in cancer patients

Abstract: The Regan isoenzyme (RI) of alkaline phosphatase is interesting because it is indistinguishable from the placental isoenzyme with regard to stability to heat, inhibition by L-phenylalanine, optimum pH. and specific reaction with rabbit antisera to placental alkaline phosphatase. The current study of 323 cancer patients was conducted at a general hospital where cancer is more frequently seen at an earlier stage of natural history and therapy than in a chronic disease hospital. A positive identification of RI was made in 39 patients (12%) by biochemical and immunologic techniques, including antibody neutralization and starch gel electrophoresis. A high incidence of carcinoma of the ovary (5 of 23) and seminoma (one of one) patients contrasted to a somewhat lower incidence in bronchogenic carcinoma (7 of 51) and breast cancer (6 of 49). Most interesting was the discovery of high RI levels in familial polyposis of the colon, and ulcerative colitis, diseases with a known cancer diathesis. In general, RI dropped sharply following treatment with effective antimetabolic chemotherapeutic agents. The ectopic production of this protein enzyme, interpreted as a consequence of derepression of the tumor cell genome, is comparable with other polypeptide hormone syndromes, or carcinoembryonic antigens, but evidences a wider variety of sites of tumor origin. Its identification in gonadal tumors and in precancerous states may represent a predeliction of such derepression for tissues concerned with germinal activity or with genetically determined cancer diathesis, respectively.