Showing papers on "Alveolar hydatid disease published in 1996"

Immunological parameters associated with susceptibility or resistance to alveolar hydatid disease in Yupiks/Inupiats.

Abstract: Alveolar hydatid disease (AHD) is a serious and often fatal disease with a relatively high prevalence among the Alaska native Yupik/Inupiat population. In a few patients, however, a spontaneous cure of the disease has been shown by demonstrating the presence of dead metacestode lesions. The present study shows a comparative analysis of the humoral (antibody activity to two different antigens: Em2-antigen and recombinant II/3-10-antigen) immune response and a respective immunogenetic background (HLA-DR typing) in (i) "susceptible" patients who had a still active intrahepatic metacestode and (ii) "resistant" patients who were shown to be spontaneously cured by presenting dead and calcified lesions. Control groups included relatives who were genetically closely related and less related cohabitants of the same villages. Antibody levels in the Em2- and the II/3-10-ELISA were high for patients who had still active lesions and low (Em2-ELISA) or negative (II/3-10-ELISA) for cured patients with dead lesions. Comparative HLA-DR analyses between infected and non-infected Yupiks/Inupiats revealed a slight tendency for susceptibility markers respective to the HLA-DRB1*0901 and HLA-DRB1*1601,02 genes.

The mechanism of distant metastases of alveolar hydatid disease

Abstract: The mechanism of the distant metastasis of echinococcosis was investigated using jirds (Meriones unguiculatus) by inoculation of fractions obtained from echinococcal lesions formed in the peritoneal cavity of cotton rats (Sigmoid hispidus). Protoscoleces, cysts, and germinal cells were fractionated from the peritoneal lesions of the cotton rats injected peritoneally with echinococcal germinal cells. Each fraction (protoscoleces; 500 pieces, cysts; 50 pieces, germinal cells; 2 x 10(7) cells) suspended in 0.2 ml of PBS was injected into either the left inguinal vein (IV group) or the mesenteric vein (MV group) of seven week-old jirds. Eight weeks after the injection, the jirds were sacrificed and examined macroscopically and microscopically. In IV group, one of 10 jirds had echinococcal lesions in lung, bilateral adrenal, brain, para-aortic lymph node and left inguinal lymph node by inoculation of only germinal cells. Another one had lung lesions formed by cysts inoculated. In MV group, both intrahepatic and pulmonary echinococcal lesions by inoculation of germinal cells were observed in 3 out of 5 jirds. Cysts inoculated formed intrahepatic lesions in all 5 jirds. However, protoscoleces inoculated through both routes never formed echinococcal lesions in any organs. The typical lesions of echinococcosis were observed in all lesions without protoscoleces and calcification. These results indicate that germinal cells in intrahepatic echinococcal lesions might invade into the intrahepatic vein and metastasize to other organs.