Topic
Amylase
About: Amylase is a research topic. Over the lifetime, 14164 publications have been published within this topic receiving 296069 citations.
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TL;DR: The addition of Tween 80 and sucrose monopalmitate, nonionic surfactants, to fungal cultures resulted in marked increases in yields of the enzymes cellulase, amylase, sucrase, beta-1 --> 3 glucanase, xylanase, purine nucleosidase, and benzoyl esterase.
Abstract: The addition of Tween 80 and sucrose monopalmitate, nonionic surfactants, to fungal cultures resulted in marked increases in yields of the enzymes cellulase, amylase, sucrase, beta-1 --> 3 glucanase, xylanase, purine nucleosidase, and benzoyl esterase. The action appears to be an effect of the surfactant on cell permeability.
270 citations
TL;DR: In this article, three conceptual action patterns of α-amylase hydrolysis of amylose have been considered: single chain, multichain, and multiple attack, and curves were obtained relating the drop in amyloses-iodine color to the increase in reducing value for amylolysis by human salivary (HS), porcine pancreatic (PP), Aspergillus oryzae (AO), and 1 m H 2 SO 4.
269 citations
TL;DR: The results suggest that <5% of the HMO are digested in the intestinal tract, suggesting that HMO may play a role as prebiotics or as factors influencing the local immune system of the intestine in breast-fed infants.
Abstract: In examining the functional aspects of human milk oligosaccharides (HMO), it is not known whether they are digested during the passage through the infant's gastrointestinal tract. HMO were prepared from individual milk samples (n = 6) and separated into neutral and acidic compounds by chromatography. These oligosaccharide fractions were studied for their digestibility by human salivary amylase, porcine pancreatic amylase and brush border membrane vesicles (BBMV) isolated from porcine small intestine; we also examined the effect of low pH on these structures. The characterization of HMO and their digestion products was performed by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) as well as TLC. It was shown that neither salivary amylase nor pancreatic amylase cleaved HMO. Only after a 2-h incubation with BBMV were slight modifications of the HMO observed. HPAEC-PAD analysis revealed two new components within the neutral oligosaccharide fractions; these were characterized by mass spectrometric analysis as lacto-N:-triose and galactose. Only lacto-N:-triose was present within digestion assays of oligosaccharides, which did not contain fucosyl or N:-acetylneuraminic acid residues. These results suggest that <5% of the HMO are digested in the intestinal tract. Hence, HMO may play a role as prebiotics or as factors influencing the local immune system of the intestine in breast-fed infants.
269 citations
TL;DR: The surface weighted mean diameter of separated granules was in good agreement with predictions from Stokes' law of sedimentation, consistent with a role for surface area in controlling amylase digestion rates as mentioned in this paper.
269 citations
TL;DR: An in vitro system of guinea pig pancreatic lobules convenient for the study of secretory processes is described in this paper and the kinetics of the appearance of three enzyme activities and four potential proteolytic activities into the incubation medium were studied.
264 citations