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Amylase
About: Amylase is a research topic. Over the lifetime, 14164 publications have been published within this topic receiving 296069 citations.
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77 citations
TL;DR: The cell wall of Chlorella is composed of up to 80% carbohydrates including cellulose and if cells were washed with chilled ethanol, cold dried, and grounded the biomass hydrolysis yields increased to 23.3% and 18.4% glucose and 24.2% reducing sugars for C. homosphaera and C. zofingiensis.
Abstract: The cell wall of Chlorella is composed of up to 80% carbohydrates including cellulose. In this study, Chlorella homosphaera and Chlorella zofingiensis were evaluated as source of fermentable sugars via their cell wall enzymatic degradation. The algae were cultivated in inorganic medium, collected at the stationary growth phase and centrifuged. The cell pellet was suspended in citrate buffer, pH 4.8 and subjected to 24 hours hydrolysis at 50°C using a cellulases, xylanases, and amylases blend. The measurement of glucose and reducing sugars concentration in the reaction mixture supernatant, on a dry biomass base, showed hydrolysis yields of 2.9% and 5.03% glucose and 4.8% and 8.6% reducing sugars, for C. homosphaera and C. zofingiensis, respectively. However if cells were washed with chilled ethanol, cold dried, and grounded the biomass hydrolysis yields increased to 23.3% and 18.4% glucose and 24.5% and 19.3% reducing sugars for C. homosphaera and C. zofingiensis, respectively.
77 citations
TL;DR: A maltotriose-producing α-amylase from Thermobifida fusca NTU22 was purified 7.2-fold as measured by specific activity from crude culture filtrate by ammonium sulfate fractionation, Sepharose CL-6B and DEAE-SepharoseCL- 6B column chromatography.
77 citations
TL;DR: In this paper, the effect of ball-mill treatment on physical properties and molecular change of maize starch granule was investigated, and the results showed that starch granules lost smoothness on surface and became rough, even though their changing speed was different among the three species.
Abstract: Effect of ball-mill treatment on physical properties and molecular change of maize starch granule was investigated. Ball-mill treatment was done by rotary type mill, and species of maize starch are normal, waxy and high amylose (amylo). Running time of the treatment is 0–320 h. Starch granules loss smoothness on surface and became rough, even though their changing speed was different among the three species. But, they retained whole figure and size after 320 h treatment in the all cases. Amylase susceptivity and water absorption activity were measured. Structural change of starch components was compared among the three species with X-ray diffraction, DSC and GPC. High Performance An-ion Exchange Chromatography (HPAEC) pattern of debranched sample treated with ball-mill for 320 h showed that formation of very short chain in amylopectin is little. 13C solid-NMR spectra suggest that disruption of molecules of amylopectin and amylose with ball-mill might occur at their glycosidic linkage. However, very slight radical was observed by Electron Spin Resonance spectroscopy (ESR) in the case of 320 h sample.
77 citations
TL;DR: Administration of LG2O inhibits carbohydrate digestion in the gut, and produces significant improvements in both blood glucose and insulin response following ingestion as part of the diet, and this evidence provides support for its therapeutic potential in treating diabetes mellitus and obesity.
77 citations