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Amylase

About: Amylase is a research topic. Over the lifetime, 14164 publications have been published within this topic receiving 296069 citations.


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Journal ArticleDOI
TL;DR: Both luteolin and oleanolic acid have an inhibitory effect on postprandial blood glucose increase in diabetic rats, and thus they may be a useful food supplement for the prevention of diabetes.
Abstract: Olive (Olea europaea L.) is recognized as a folk medicine for diabetes in Europe. The inhibitory action of an ethanol extract of olive leaves (OEE) on the activities of human amylases was examined in vitro. OEE inhibited the activities of α-amylases from human saliva and pancreas with IC50 values of 4.0 and 0.02 mg/ml, respectively. Two anti-α-amylase components were purified from a 50% ethanol soluble fraction of OEE using Sephadex LH-20 column chromatography. One was identified as luteolin-7-O-β glucoside and the other as luteolin-4′-O-β glucoside. The 50% ethanol insoluble fraction of OEE was dissolved in 98% ethanol and fractionated using Cosmosil C18-OPN column chromatography. The anti-α-amylase component purified by this chromatography was identified as oleanolic acid. Both luteolin and oleanolic acid have an inhibitory effect on postprandial blood glucose increase in diabetic rats. Olive leaves suppressed the elevation of blood glucose after oral administration of starch in borderline volunteers (fasting blood glucose: 110–140 mg/dl), and thus they may be a useful food supplement for the prevention of diabetes.

110 citations

Journal ArticleDOI
J. Feng1, X. Liu1, Ziqi Xu1, Y. Wang1, Jianxin Liu1 
TL;DR: Replacing soybean meal with FSBM in diet increased the activities of trypsin, lipase, and protease significantly in intestinal content of starter broilers and enhanced the protease activity of grower broilers.

110 citations

Journal ArticleDOI
TL;DR: It is concluded that starch-blocker tablets do not inhibit the digestion and absorption of starch calories in human beings.
Abstract: It has been known for more than 25 years that certain plant foods, such as kidney beans and wheat, contain a substance that inhibits the activity of salivary and pancreatic amylase. More recently, this antiamylase has been purified and marketed for use in weight control under the generic name "starch blockers." Although this approach to weight control is highly popular, it has never been shown whether starch-blocker tablets actually reduce the absorption of calories from starch. Using a one-day calorie-balance technique and a high-starch (100 g) meal (spaghetti, tomato sauce, and bread), we measured the excretion of fecal calories after normal subjects had taken either placebo or starch-blocker tablets. If the starch-blocker tablets had prevented the digestion of starch, fecal calorie excretion should have increased by 400 kcal. However, fecal calorie excretion was the same on the two test days (mean +/- S.E.M., 80 +/- 4 as compared with 78 +/- 2). We conclude that starch-blocker tablets do not inhibit the digestion and absorption of starch calories in human beings.

110 citations

Journal ArticleDOI
TL;DR: Attempts to fractionate the isoenzymes of the B family on QAE-Sephadex appear to confirm the observations made with whole parotid saliva that storage at pH 8–9 promoted the conversion of isoenzyme 4 to 2 and z.

109 citations

Journal Article
TL;DR: In this paper, a-amylase enzyme activity was observed at 55 °C and pH=5. At 75 °C, enzyme showed 90 % activity compared to 55°C.
Abstract: Summary Production of a-amylase under solid-state fermentation by Bacillus cereus MTCC 1305 has been investigated using wheat bran and rice flake manufacturing waste as substrates. With wheat bran, highest enzyme production expressed as units per mass of dry substrate ((94±2) U/g) was observed. Production parameters were optimized as inoculum size 10 % (volume per mass) and substrate:moisture ratio 1:1. Among different carbon sources supplemented, glucose (0.04 g/g) showed enhanced enzyme production ((122±5) U/g). Supplementation of different nitrogen sources (0.02 g/g) showed decline in enzyme production. Optimum a-amylase enzyme activity was observed at 55 °C and pH=5. At 75 °C, enzyme showed 90 % activity compared to 55 °C.

109 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20241
2023460
2022976
2021308
2020347
2019328