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Showing papers on "Angiogenesis published in 1981"


Journal Article
01 Aug 1981-Surgery
TL;DR: These experiments demonstrate that: (1) A hypoxic tissue gradient is mandatory for wound-healing angiogenesis, (2) when the hypoxic gradient is destroyed capillary growth cases, and (3) inspired oxygen concentrations affect the rate and density of capillary Growth.

654 citations


Journal ArticleDOI
TL;DR: The process of vascularisation was studied in transplanted astrocytomas in BD-IX rats and vascular pleomorphism and the abnormal morphological features associated with glioma vasculature were typical of the late vascular stage.

93 citations


Journal Article
TL;DR: A partially purified, low molecular-weight endothelial cell growth stimulatory factor isolated from the Walker 256 rat carcinosarcoma is demonstrated to have potent angiogenic activity in vivo.
Abstract: A corneal micropocket assay for angiogenesis in the rat eye is described in detail. With our test system, a partially purified, low molecular-weight endothelial cell growth stimulatory factor isolated from the Walker 256 rat carcinosarcoma is demonstrated to have potent angiogenic activity in vivo. The advantages and applications of our rat corneal assay are discussed.

76 citations


Journal ArticleDOI
TL;DR: It was found that cytoplasmic vesicle content was 3--6-fold greater in tumour blood vessels than in capillaries from normal brain, and may therefore play an important role in cerebral oedema.

67 citations


Book ChapterDOI
01 Jan 1981
TL;DR: The hypothesis that angiogenesis is due to the production of a factor(s) by cells involved in the process of neovascularization arose from several kinds of observations.
Abstract: New formation of vessels occurs during embryonal development and throughout the lifespan of an organism following repair processes or under certain pathologic conditions. In particular, neoplastic cell populations can grow to form a clinically evident tumor only if the host produces a vascular network sufficient to sustain tumor growth (Folkman, 1974 a, 1975; Brem et al., 1976). The hypothesis that angiogenesis is due to the production of a factor(s) by cells involved in the process of neovascularization arose from several kinds of observations: During an inflammatory response, new formation of vessels appeared when certain types of cells invaded the area; a neoplastic cell population induced the formation of a vascular network even when the tumor fragment was separated from the host by a Millipore filter; (Greenblatt and Shubik, 1968); and cell-free fluid sampled in vivo from the extracellular compartment of a growing tumor elicited an angiogenic response (Gullino, unpublished observation), as does the media of cultured neoplastic cells (Folkman, 1974 b).

43 citations


Journal Article
TL;DR: Human peripheral blood lymphocytes injected intradermally into X-ray immunosuppressed mice were tested for angiogenesis-inducing capacity, and lymphocytes both with the highest and with moderate affinity for SRBC, mixed together, evokedAngiogenesis no different from that evoked by cells of the total T lymphocyte population, suggesting that inhibitory interactions occur among T cells.
Abstract: Human peripheral blood lymphocytes injected intradermally into X-ray immunosuppressed mice were tested for angiogenesis-inducing capacity. Both T and B lymphocytes evoked angiogenesis of the same intensity. The total T cell population was fractionated into three subpopulations on the basis of their different affinities for sheep red blood cells (SRBC). Cells belonging to the subpopulation of T lymphocytes displaying moderate affinity for SRBC induced angiogenesis of the higher intensity, higher than that induced by cells of the total T lymphocyte population. However, lymphocytes both with the highest and with moderate affinity for SRBC, mixed together, evoked angiogenesis no different from that evoked by cells of the total T lymphocyte population, suggesting that inhibitory interactions occur among T cells.

24 citations


Journal ArticleDOI
01 Jan 1981-Cancer
TL;DR: Two angiosarcomas of the heart, both arising from the right atrium, were studied with the electron microsope and Ultrastructural characteristics of angiomatous development were demonstrated both at the better differentiated vascular areas and at the less differentiated, spindle‐cell foci.
Abstract: Two angiosarcomas of the heart, both arising from the right atrium, were studied with the electron microscope. Ultrastructural characteristics of angiomatous development were demonstrated both at the better differentiated vascular areas and at the less differentiated, spindle-cell foci. Both tumors were dominated by immature endothelial cells. These cells were associated with primitive mesenchymal cells and transitional forms of pericytes. Intracytoplasmic lumen formation and junctional specialization with neovascular development are unique features of angiogenesis in these tumors. Ultrastructural studies may provide useful diagnostic features separating a true malignant vascular neoplasm of endothelial cell origin from other forms of mesenchymal neoplasia that may have a prominent stromal vascular component.

20 citations


Journal Article
TL;DR: In this article, the capacity to induce new formation of capillaries was tested in cells attached to plastic coverslips and it was observed that within a few weeks after implantation, these cells showed an angiogenic capacity about 5-fold greater than that of the small coverslip cells.
Abstract: The capacity to induce new formation of capillaries was tested in cells attached to plastics. It is known that large plastic coverslips implanted s.c. in CBA mice produce sarcomas more rapidly and in a greater number than do small coverslips. We observed that within a few weeks after implantation the cells attached to the large coverslips showed an angiogenic capacity about 5-fold greater than that of the cells attached to the small coverslips. Months before a sarcoma was evident, angiogenesis induced by the cells attached to the large coverlips predicted the high risk of neoplastic transformation by large coverslips.

20 citations


Journal ArticleDOI
Haruo Matsuno1
TL;DR: TAF samples obtained from glioma cells which had been irradiated before TAF extraction, also showed angiogenic activity, and TAF was present in the supernatants of long-term cultured human CNS tumor cell lines and primary cultures of meningiomas.
Abstract: The angiogenic activity in the supernatants of cultured cells derived from human CNS tumors was examined by a bioassay method using chick chorioallantoic membrane (CAM). A quantitative assessment was designed to obtain a more accurate evaluation of angiogenesis on CAM. Three out of five glioma cell lines, three out of four primary cultures of meningiomas and one metastatic tumor cell line showed angiogenic activity in the culture supernatants. The angiogenic activity was destroyed by ribonuclease or heating at 80°C for 30 minutes but remained intact with exposure to trypsin, deoxyribonuclease or heating at 56°C for 30 minutes. TAF samples obtained from glioma cells which had been irradiated (2, 500 R) before TAF extraction, also showed angiogenic activity. TAF was present in the supernatants of long-term cultured human CNS tumor cell lines and primary cultures of meningiomas. Thus, the nature of TAF may related to RNA of the tumor cells and the TAF producing ability of these cells is apparently not inhibited by irradiation.

10 citations



01 Jan 1981
TL;DR: The authors consider three successive phenomena in the experimental Ca H tumor evolution: a first stage of tumor resistance due to cytotoxic cells in the presence of some suppressor cells, a second stage when suppressor activity is predominantly observed, and a final stage of lymphocyte-mediated immunostimulation.
Abstract: The authors consider three successive phenomena in the experimental Ca H tumor evolution A first stage of tumor resistance due to cytotoxic cells in the presence of some suppressor cells; a second stage when suppressor activity is predominantly observed, and a final stage of lymphocyte-mediated immunostimulation Vasodilatation and angiogenesis are probably important during third stage