Topic
Apical cytoplasm
About: Apical cytoplasm is a research topic. Over the lifetime, 1080 publications have been published within this topic receiving 36131 citations.
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TL;DR: The present results indicate that the SCOs of rodent species contain CGRP that may be in part synthesized by ependymocytes themselves, and a species difference in the C GRP distributions of rodents SCOs may therefore imply its different synthesis rates or release pathways.
Abstract: For the first time we showed the presence of CGRP immunoreactivity in the SCO of golden hamsters, Wistar rats and Mongolian gerbils. In hamsters, the intense CGRP-like immunoreactivity was detected in the whole SCO, including Reissner's fiber- and granule-like structures at the ventricular surface of the SCO. The CGRP-positive hypendymal cells were frequently in contact with local blood vessels and some of them were situated intimately to the leptomeningeal space. In the SCOs of rats and gerbils, only the supranuclear area and apical cytoplasm of the ependymal cells were positive for CGRP, whereas the basal pole of the cells and the hypendyma were CGRP-negative. The existence of CGRP in rodents SCO was confirmed by the expression of CGRP mRNA in rat SCO by RT-PCR. The present results indicate that the SCOs of rodent species contain CGRP that may be in part synthesized by ependymocytes themselves. A species difference in the CGRP distributions of rodents SCOs may therefore imply its different synthesis rates or release pathways.
1 citations
TL;DR: There is a close relationship between distributional changes in LBs and the transformation and differentiation of blastomeres in mouse, rabbit and bovine embryos, according to acridine orange (AO) staining.
Abstract: Distributional changes in lysosome-like bodies (LBs) in mouse, rat, rabbit and bovine embryos during the course of blastocyst formation were studied with acridine orange (AO) staining. AO-stained LBs were distributed throughout the cytoplasm of all round blastomeres in untransformed 8- and 16-cell mouse embryos, 32-cell rabbit embryos and 16-cell bovine embryos, whereas they were observed from the nucleus to the apical cytoplasm of all round blastomeres in 8-cell rat embryos. In morulae of these animals, LBs were present in the entire cytoplasm of inner round blastomeres, and were localized around the nucleus in outer flattened (mouse) and cuboidal (rabbit and cow) blastomeres or the apical cytoplasm in outer flattened (rat) blastomeres.In blastocysts, LBs were present in the entire cytoplasm of inner-cell-mass cells and around the nucleus of trophoblast cells. Such distribution of LBs in mouse embryos was also observed in those developed in vitro. From these results, it was suggested that there is a close relationship between distributional changes in LBs and the transformation and differentiation of blastomeres in mouse, rabbit and bovine embryos.
1 citations
TL;DR: While there were no significant changes in lung weights and respiratory epithelial thicknesses between all studied groups, statistical analysis showed a significant alteration in the thickness of interalveolar septa in the St and St+GdCl3 group compared to the control groups (P<0.001).
1 citations
TL;DR: Seasonal variations in the secretory activity of the subcommissural organ of snakes and turtles was studied to suggest a decreased synthesis and release of secretory material in the SCO of lethargic reptiles.
1 citations
01 Jun 2014
TL;DR: The stomach of the farmed African catfish was investigated in this study to establish its basic anatomy and produce baseline data for further investigative research, help fish clinicians in pathology and aid nutritionists in feed management.
Abstract: The stomach of the farmed African catfish (Clarias gariepinus, Siluriformes: clariidae) was investigated in this study to establish its basic anatomy, as there is scanty information available on the literature. It produced to baseline data for further investigative research, help fish clinicians in pathology and aid nutritionists in feed management. After cropping from a commercial aquaculture, apparently healthy fish were immobilized and euthanized by using chloroform. The samples were dissected and slices of cardiac, fundic and pyloric stomach processed through routine histological procedures. Grossly, the stomach was J-shape. Cranial to the stomach was the oesophagus but no sphincter was seen separating them. Caudally, a pyloric sphincter separated the stomach from the proximal intestine. The stomach contained three regions cardiac, fundic and pyloric. The entire stomach surface was lined by a simple columnar epithelium containing mucin at the apical cytoplasm. Only the cardiac and fundic regions contained gastric glands in the lamina propria, but the glands were more developed in the fundic region. The tunica muscularis contained smooth muscle cells in an inner circular and outer longitudinal orientation. Mucin histochemistry revealed the presence of only neutral mucin in the stomach. The results obtained reSumen
1 citations