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Apotome

About: Apotome is a research topic. Over the lifetime, 9 publications have been published within this topic receiving 68 citations.

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Journal ArticleDOI
TL;DR: The anisotropy of the ApoTome's resolution was found to be negligible for standard applications while its capability to resolve fine structures within stained tissue slices is limited to one or two cell layers and thus worse than in the cLSM.
Abstract: The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological specimens. Here we compare the resolution of the ApoTome (commercial SIM by Zeiss) to that achieved by a cLSM (Zeiss LSM 510). If fluorescent beads are used as test objects, then the ApoTome will achieve a lower axial resolution than the cLSM. In contrast to that, its lateral resolution scores slightly better. If subresolution homogeneous fluorescent layers are used as test objects, then the ApoTome will achieve a higher axial resolution than the cLSM. The ApoTome's axial resolution is homogeneous over the field-of-view while that of the cLSM changes markedly. Finally, the anisotropy of the ApoTome's resolution was found to be negligible for standard applications while its capability to resolve fine structures within stained tissue slices is limited to one or two cell layers and thus worse than in the cLSM.

43 citations

Journal ArticleDOI
TL;DR: The ApoTome images localize the nuclear and membranous expressions of p63 and E‐cadherin, respectively, with remarkable specificity, which clearly differentiate the early and advanced stages of oral submucous fibrosis based on their cytoplasmic and membrane location.
Abstract: Precise histopathological localization of E-cadherin and p63 is of immense importance in understanding the integrity of oral mucosal stratified epithelium in normal and diseased conditions. Necessarily immunohistochemical imaging should have minimum bleaching impact on the dyes and ability to produce clear and crisp images. Here ApoTome provides an alternative with metal halide light source and structured illumination under the assistance of grids, along with integrated image processing modality to generate crisp images with digital interface. The current study demonstrates the applicability of such microscopic system in capturing fluorescence images of immunohistochemical sections of normal and precancerous biopsies in respect to the expression of p63 and E-cadherin in the epithelial cells. The ApoTome images localize the nuclear and membranous expressions of p63 and E-cadherin, respectively, with remarkable specificity. The findings on E-cadherin expression have enormous diagnostic significance as these images clearly differentiate the early and advanced stages of oral submucous fibrosis based on their cytoplasmic and membranous location. Thus, this study clearly depicts a remarkable performance of ApoTome with diagnostic significance.

8 citations

Journal Article
TL;DR: The development of the pupal dorsal apotome was examined and compared in representatives of the families Culicidae, Chaoboridae, Corethrellidae, and Dixidae to show the differences and similarities between taxa.
Abstract: The development of the pupal dorsal apotome was exam/ned and compared in representatives of the families Culicidae, Chaoboridae. Corethrellidae. and Dixidae. Illustrations of the pupal dorsal apotome for 73 species. in 25 genera. are provided to show the differences and similarities between taxa. The 4th-instar larval dorsal apotome is compared and illustrated for the 3 subfamilies of Culicidae. The phylogenetic signifi- cance of the dorsal apotome of the pupae and 4th-instar larvae is discussed.

7 citations

Journal ArticleDOI
25 Aug 2013-Grana
TL;DR: In this article, an executable for the automated segmentation and cropping of pollen grains from microscope images based on pixel intensity values was developed, which works with images taken using transmitted-light, widefield-fluorescence, structured illumination (Apotome), and includes a novel approach for cropping the Apotome Z-stack.
Abstract: Image analysis methods have the potential to increase the accuracy and rates of data collection in palynological research. Automated segmentation of pollen grains is a method that would facilitate image-based palynological analysis by creating large reference image libraries. We developed an executable for the automated segmentation and cropping of pollen grains from microscope images based on pixel intensity values. Our method works with images taken using transmitted-light, widefield-fluorescence, structured illumination (Apotome), and includes a novel approach for cropping the Apotome Z-stack. The system crops pollen grains from sampled fields of view with ~97% recall and ~97% precision for transmitted-light and widefield-fluorescence images, and ~90% recall and ~89% precision for Apotome fluorescence images. Results differed between different imaging wavelengths for fluorescence images, with Apotome images showing the greatest difference between red and green emission wavelengths. Recall in cr...

6 citations

Journal ArticleDOI
TL;DR: This paper presents an image restoration approach suitable for processing raw incoherent-grid-projection SIM data with a low fringe contrast, and shows results with improved signal-to-noise ratio (SNR) and optical sectioning compared to the results obtained from existing methods.
Abstract: The performance of structured illumination microscopy (SIM) is hampered in many biological applications due to the inability to modulate the light when imaging deep into the sample. This is in part because sample-induced aberration reduces the modulation contrast of the structured pattern. In this paper, we present an image restoration approach suitable for processing raw incoherent-grid-projection SIM data with a low fringe contrast. Restoration results from simulated and experimental ApoTome SIM data show results with improved signal-to-noise ratio (SNR) and optical sectioning compared to the results obtained from existing methods, such as 2D demodulation and 3D SIM deconvolution. Our proposed method provides satisfactory results (quantified by the achieved SNR and normalized mean square error) even when the modulation contrast of the illumination pattern is as low as 7%.

4 citations

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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20201
20181
20131
20121
20111
20093