Topic
Aqueous normal-phase chromatography
About: Aqueous normal-phase chromatography is a research topic. Over the lifetime, 2082 publications have been published within this topic receiving 48642 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: Hydrophilic-interaction chromatography fractionations resemble those obtained through partitioning mechanisms, and the chromatography of DNA, in particular, resembles the partitioning observed with aqueous two-phase systems based on polyethylene glycol and dextran solutions.
1,761 citations
1,306 citations
TL;DR: In this article, the effect of solute ionization on the retention of weak acids, bases, and ampholytes on octadecylsilica was investigated both theoretically and experimentally.
Abstract: The effect of solute ionization on the retention of weak acids, bases, and ampholytes on octadecylsilica was investigated both theoretically and experimentally. The retention was at tributed to a reversible association of the solutes with the hydrocarbonaceous ligand of the stationary phase. A phe nomenological treatment of the corresponding equilibria was developed for various types of ionogenic substances. The energetics of the association process was analyzed in a rig orous fashion in the light of the solvophobic theory and a semi-empirical extension of the Debye-Hiickel theory to high ionic strength. The predicted effect of solute ionization on the capacity factors was substantiated by experimental data. The observed dependence of the capacity factors on the ionic strength of the eluent and the hydrophobic surface of the solute molecules showed good agreement with the theory. The ad vantages of the technique in the separation of biological sub stances are illustrated.
597 citations
Book•
20 Sep 1988
TL;DR: Theoretical principles for pump preparation of equipment up to sample detectors columns and stationary phases column packing and regeneration are discussed in this paper, along with a review paper on the separation of individual classes of compounds commercially available stationary phases.
Abstract: Theoretical principles pumps preparation of equipment up to sample injection detectors columns and stationary phases column packing and regeneration HPLC packing and regeneration HPLC column tests adsorption chromatography reversed-phase chromatography liquid-liquid partition chromatography chromatography with chemically bonded phases ion-exchange chromatography ion-pair chromatography ion chromatography size exclusion chromatography affinity chromatography choice of method analytical HPLC preparative HPLC separation of enantiomers new prospects review papers on the separation of individual classes of compounds commercially available stationary phases for HPLC.
514 citations
474 citations