Showing papers on "Arabidopsis published in 1980"

Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) heynh.

Abstract: In Arabidopsis thaliana 37 independent irradiation or EMS induced mutants were isolated which have an absolute or almost absolute gibberellin (GA) requirement for germination and successive elongation growth. These are called 'non-germinating GA-dwarfs', since without further addition of GA they develop into typical GA-dwarfs, being dark green, stunted and sterile. However, with repeated GA-treatment they develop into fertile plants with a completely wild type phenotype, or nearly so. In addition, 19 independently induced 'germinating GA-dwarfs' were obtained, i.e. mutants which do germinate without GA but develop into typical GA-dwarfs. With repeated GA-treatment these too grow to become completely wild type phenotypes, or nearly so. 'Germinating dwarfs' have been found by previous authors in a number of other plant species. The 'non-germinating dwarfs' form a new class of mutants. The system of non-germinating mutants offers a resolving power unique in higher plants, so that self-detecting rare events like induced revertants or intragenic recombinants can be efficiently screened for.The 56 GA-sensitive mutants represent mutations at 5 loci, located on three of five Arabidopsis chromosomes. At three of the five loci both mutant classes were represented in similar frequency ratio's, whilst at the other two loci only germinating dwarfs were found.

Identification of carcinogens by mutagenicity for arabidopsis

Abstract: Of 56 known or suspected carcinogens and neoplastic chemicals of diverse structures, previously tested with animals, approx, 88% were found to be mutagenic for the plant Arabidopsis. This correlation is about as good as that observed in using the Ames test of Salmonella/microsomes. But the plant assay does not require previous activation of the chemicals or any other special treatment. The false negatives in Arabidopsis do not entirely overlap those of the bacterial assay. This plant assay is faster and less expensive than most direct mutagenic tests with other higher eukaryotes; also it encompasses a broad range of genetic alterations involving thousands of loci with many sites.

Arabidopsis Assay of Environmental Mutagens

Abstract: In the past, Arabidopsis assays have been employed for testing the mutagenic effects of a variety of chemicals Although it is potentially useful for determining mutagenic hazards of complex mixtures, this species has not been much used for this purpose