Arecoline

About: Arecoline is a research topic. Over the lifetime, 744 publications have been published within this topic receiving 16015 citations. The topic is also known as: methylarecaiden & methylarecaidin.

Interleukin‐13 contributes to the occurrence of oral submucosal fibrosis

Abstract: Oral submucous fibrosis (OSF) is a chronic progressive fibrosis disease that affects in oral mucosal tissues. Interleukin (IL)‐13 has been implicated in the development of fibrosis in multiple organs. Indeed, it contributes to diseases such as pulmonary fibrosis, liver cirrhosis among others. Currently, its expression in OSF and the specific mechanisms are not well understood. The aim of this study was to investigate the role of IL‐13 in OSF and further explore whether IL‐13 regulates—polarization of M2‐macrophages in OSF. Initially, in the tissues of patients with OSF, we observed a high expression of M2‐macrophages and IL‐13 protein. Additionally, we found a correlation between the expression of IL‐13 and the stage of OSF. Arecoline inhibited the proliferation of fibroblasts (FBs) and promoted IL‐13 production in vitro. Furthermore, our observations revealed that M2‐macrophages increased upon co‐culturing M0‐macrophages with supernatants containing the IL‐13 cytokine. In conclusion, our study demonstrated that arecoline stimulates FBs leading to increased secretion of IL‐13, which in turn IL‐13 leads to polarization of M2‐macrophages and promotes the occurrence of OSF. This suggests that IL‐13 may be a potential therapeutic target of OSF.

Response surface optimization of supercritical extraction process of Arecoline

Abstract: Arecoline was obtained with the methods of microwave pretreatment- supercritical CO2 fluid extraction from areca nut. The content of the arecoline which was used as evaluating criteria, was determined by HPLC. Based on the results of single factor experiments, using response surface methodology(RSM) to fit a quadratic polynomial regression model as a response to three independent variables which had significant effects on the arecoline yield. Results showed that the yield of arecoline under microwave pretreatment could improve obviously, the optimal values of technological parameters was as follows: the extraction pressure was 33 MPa; the extraction temperature was 63 ℃; the microwave power was 480 W. Under such conditions, the yield of arecoline was up to (0.448±0.007)% (n=3), which was very close to the predicted value of 0.452%.