Showing papers on "Aspergillus niger published in 1971"
TL;DR: It is suggested that the two forms of glucoamylase are isoglycoenzymes, probably due to a difference in the number of amide groups or glycosylaminically linked carbohydrate units in the polypeptide chains.
88 citations
69 citations
43 citations
TL;DR: In this article, the glucoamylase pattern, which is characterised by a relatively uniform erosion of the surface is discussed in comparison with that of alpha amylase and possible reasons for the differences are suggested.
Abstract: Starch granules attacked by glucoamylase of fungal origin show a pattern of erosion which is different from that resulting from wheat alpha-amylase attack. The glucoamylase pattern, which is characterised by a relatively uniform erosion of the surface is discussed in comparison with that of alpha-amylase and possible reasons for the differences are suggested.
39 citations
TL;DR: Data presented indicate that among the subcellulr fractions, only microsomers are active in the mannose transfer reactions, and the efficiency of this enzyme-aceptor system is discussed.
Abstract: A glycoprotein-mannosyltransferase, utilsing an endogenous acceptor found in Aspergillus niger is herein characterized. Data presented indicate that among the subcellulr fractions, only microsomers are active in the mannose transfer reactions.
The enzyme catalising the transfer requires Mn++ or Mg++ (1–2 mM) and has an optimum pH of 6.8 and an optimum temperature of 22°.
It exibits a Km of 0.2 μM for GDP-mannose. The transfer is inhibited by ATP, CTP and also bt GTP or its structural analogue β-γ-methlene-guanosine triphosphate, but AMP or GDP have only a slight inhibitory action.
Triton X-100 causes of 2- to 3-fold enhancement of incorporation of mannose when a suitable detergent: protein ratio is applied.
The efficiency of this enzyme-aceptor system is discussed.
31 citations
21 citations
TL;DR: In this paper, the pH-dependent photooxidation of glucoamylase I in the presence of methylene blue results in the abolition of the enzymic activity, being more rapid at high pH.
19 citations
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17 citations
TL;DR: In soil treated with various doses of carbon disulphide, allyl alcohol or formalin, Penicillium notatum was consistently the most tolerant organism and was followed in tolerance by Aspergillus niger, A. niger and A. flavus.
Abstract: In soil treated with various doses of carbon disulphide, allyl alcohol or formalin, Penicillium notatum was consistently the most tolerant organism. It was followed in tolerance by Aspergillus niger, A. fumigatus, Altemaria alternata and Cladosporium herbarum in the case of carbon disulphide; by A. niger, Trichoderma, A. ochraceus and C. herbarum in the case of allyl alcohol; and by Trichoderma and A. flavus in the case of formalin. Trichoderma became the dominant recolonizer of Egyptian alkaline soils fumigated with allyl alcohol and formalin, of which it was highly and moderately tolerant, respectively, constituting after some doses 100% of the recolonizing fungus flora. In soil fumigated with carbon disulphide, of which it showed low tolerance, itgrew after a dose of 0·08 ml faster than any other fungus, as indicated by its recolonization ability (ratio of count 36 days to that of 1 day after fumigation), but its surviving inoculum potential was probably too low for it to gain dominance over other more tolerant fungi, such as P. notatum, A. niger and A. flavus .
15 citations
Journal Article•
TL;DR: In vitro studies using Aspergillus niger isolated from the patient showed that these organisms could bind and concentrate 85Sr, and apparently the soft-tissue localization of 85SR in this patient was due to As pergillosis within these tissues.
Abstract: A case of soft-tissue localization of 85Sr in a patient with cancer and aspergillosis is presented. Autopsy tissue specimens revealed that the soft tissues infected by Aspergillus niger had 14 times more radioactivity than comparable uninfected soft tissues obtained from the same patient. In vitro studies using Aspergillus niger isolated from the patient showed that these organisms could bind and concentrate 85Sr. Apparently the soft-tissue localization of 85Sr in this patient was due to Aspergillus niger within these tissues.
TL;DR: The specific activities of certain enzymes of the Embden-Meyerhof-Parnas and pentose phosphate pathways varied in cultures of Aspergillus niger in relation to the stage of the growth cycle and the nature of the growing medium.
Abstract: The specific activities of certain enzymes of the Embden-Meyerhof-Parnas (EMP) and pentose phosphate (PP) pathways varied in cultures of Aspergillus niger in relation to the stage of the growth cycle and the nature of the growth medium. At 24 h from spore inoculation the levels of PP pathway enzymes glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and transketolase were higher in extracts from non-sporulating than from sporulating mycelium. Correspondingly, the levels of most EMP pathway enzymes were higher in extracts from sporulating than from non-sporulating mycelium. These fluctuations in enzyme levels occurred well in advance of any morphological evidence of sporulation. At later stages of growth (48–96 h) the levels of the enzymes were similar in extracts from both types of mycelium. Radiorespirometric patterns for the oxidation of specifically labelled [14C] glucose implied that the EMP and PP pathways were active in both types of mycelium. The contribution of the PP pathway was highest in young non-sporulating mycelium whereas the contribution of the EMP pathway was highest in sporulating mycelium of corresponding age.
TL;DR: Cultures of Aspergillus niger growing in a sporulation medium produce more ethanol than cultures in a non-sporulation medium and it is considered that this increased ethanol production is related to a stimulation of the Embden-Meyerhof-Parnas pathway during sporulation.
Abstract: Cultures of Aspergillus niger growing in a sporulation medium produce more ethanol than cultures in a non-sporulation medium. It is considered that this increased ethanol production is related to a stimulation of the Embden-Meyerhof-Parnas pathway during sporulation. Studies with metabolic inhibitors suggest that sporulation depends on the functioning of the complete glycolytic pathway and the tricarboxylic acid cycle. Addition of glycolytic intermediates to cultures on non-sporulation medium induces sporulation.
TL;DR: Series of 1,3-dihalogeno-5-nitrobenzenes, 3- and 3,5-halogenoanilines, and 2,6-diphenylines tested for fungitoxicity against Aspergillus niger, A. oryzae, Trichoderma viride, Myrothecium verrucaria, and Trichophyton mentagrophytes were found to possess sufficient activity to warrant further study.
Abstract: Series of 1,3-dihalogeno-5-nitrobenzenes, 3- and 3,5-halogenoanilines, and 2,6-dihalogeno-4-nitroanilines were tested for fungitoxicity against Aspergillus niger, A. oryzae, Trichoderma viride, Myrothecium verrucaria, and Trichophyton mentagrophytes in shaken culture by using Sabouraud dextrose broth enriched with yeast extract as the test medium. 1,3-Dichloro-5-nitrobenzene, 1,3-dibromo-5-nitrobenzene, 3-iodoaniline, 3,5-dichloroaniline, and 3,5-dibromoaniline were found to possess sufficient activity, compared with 8-quinolinol, to warrant further study.
TL;DR: Aus 6 verschiedenen Heterokaryen wurden 36 diploide Stamme von A. niger erhalten, deren Fahigkeit zur Citronensauresynthese auf Rubenmelassenahrmedium uberpruft wurde.
Abstract: Aus 6 verschiedenen Heterokaryen wurden 36 diploide Stamme von A. niger erhalten, deren Fahigkeit zur Citronensauresynthese auf Rubenmelassenahrmedium uberpruft wurde. Unter den aus haploiden Elternstammen mit schwacher oder mittlerer saure-bildender Aktivitat entstandenen Diploiden uberwogen Stamme, die weniger oder nur geringfugig mehr Citronensaure produzierten als der bessere Ausgangsstamm. Im Gegensatz dazu synthetisierten alle Diploiden aus Stammen mit verhaltnismasig hoher saurebildender Aktivitat mehr Citronensaure (8,3–17,7%) als die Ausgangsstamme.
TL;DR: Incubation of androst-4-ene-3,17-dione with Aspergillus niger, ATCC 9142, gives 18-hydroxyandrost- 4-enes-3-17-Dione.
Abstract: Incubation of androst-4-ene-3,17-dione with Aspergillus niger, ATCC 9142, gives 18-hydroxyandrost-4-ene-3,17-dione.
TL;DR: The results of the experiments reported here demonstrate that major changes in metabolism are taking place in mycelium which will ultimately differentiate into sporingmycelium, long before nutrient exhaustion has occurred in the growing medium.
Abstract: The concentrations of the intermediates of the EMP pathway and adenosine phosphates varied during the growth of Aspergillus niger as a function of the stage of the life-cycle and of the growth medium The concentrations of the EMP intermediates were considerably higher in extracts of mycelium from sporulation medium than from non-sporulation medium at the 24 h stage of development, well in advance of morphological evidence of sporulation At later stages of growth (48–72 h) the levels of the intermediates were relatively similar Throughout the growth cycles the concentrations of the adenosine phosphates were, in general, higher in extracts of mycelium from sporulation medium than from non-sporulation medium The results of the experiments reported here demonstrate that major changes in metabolism are taking place in mycelium which will ultimately differentiate into sporing mycelium, long before nutrient exhaustion has occurred in the growing medium
TL;DR: Mannitol remained present in appreciable amounts in these four fungi throughout the whole period of autolysis described, whereas arabitol disappeared from B. cinerea and fromA.
Abstract: Botrytis cinerea, Colletotrichum gloeosporioides, Aspergillus niger andPenicillium griseofulvum were grown in flasks as stationary cultures in the ordinary Czapek-Dox medium at 24° C in the dark for long periods of time At convenient intervals during autolysis samples of mycelium were harvested and its polyol content determined The loss of mycelial mannitol during autolysis reached to a little more than 60 %, more than 70 %, to nearly 80 % and 90 % forC gloeosporioides, P griseofulvum, A niger andB cinerea, respectively Seventy per cent of the arabitol contained inP griseofulvum and 85 % of the arabitol inC gloeosporioides disappeared during autolysis Mannitol remained present in appreciable amounts in these four fungi throughout the whole period of autolysis described, whereas arabitol disappeared fromB cinerea and fromA niger during the process
Journal Article•
Journal Article•
02 Apr 1971