Topic
Bacteria
About: Bacteria is a research topic. Over the lifetime, 23676 publications have been published within this topic receiving 715990 citations. The topic is also known as: eubacteria.
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TL;DR: This study was conducted to examine the ability of selected dairy strains of lactic acid bacteria to remove aflatoxin B1 from liquid media and found that both Lactobacillus rhamnosus strain GG and LC-705 can significantly remove AFB1 when compared with that by other strains of either Gram-positive or Gram-negative bacteria.
429 citations
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TL;DR: Various workers attempting to stain the halophilic bacteria which are commonly responsible for the red discoloration of salted hides and salted fish have been unanimous in reporting major difficulties.
Abstract: Various workers attempting to stain the halophilic bacteria which are commonly responsible for the red discoloration of salted hides and salted fish have been unanimous in reporting major difficulties. These organisms are ordinarily grown in media containing 20 per cent or more of NaCl. They are extremely sensitive to changes in osmotic pressure and will undergo plasmoptysis immediately when immersed in distilled water. Consequently, smears for microscopical examination should be made in 20 per cent brine. Then, if a good preparation is to be obtained, a special desalting procedure must be applied before the actual staining of the organisms.
426 citations
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TL;DR: A new genus and species is described, Cycloclasticus pugetti, for these bacteria, isolated from different locations in Puget Sound, Washington, by using biphenyl as the principal carbon source, and it is revealed that these bacteria are sufficiently different from other bacteria to justify establishment of a new genus.
Abstract: Three heterotrophic bacterial strains were isolated from different locations in Puget Sound, Washington, by using biphenyl as the principal carbon source. These strains grow by using a limited number of organic compounds, including the aromatic hydrocarbons naphthalene, phenanthrene, anthracene, and toluene, as sole carbon sources. These aerobic, gram-negative rods are motile by means of single polar flagella. Their 16S rRNA sequences indicate that they are all members of the γ subdivision of the Proteobacteria. Their closest known relatives are the genera Methylobacter and Methylomonas (genera of methane-oxidizing bacteria), uncultured sulfur-oxidizing symbionts found in marine invertebrates, and clone FL5 containing 16S ribosomal DNA amplified from an environmental source. However, the Puget Sound bacteria do not use methane or methanol as a carbon source and do not oxidize reduced sulfur compounds. Furthermore, a 16S rRNA base similarity comparison revealed that these bacteria are sufficiently different from other bacteria to justify establishment of a new genus. On the basis of the information summarized above, we describe a new genus and species, Cycloclasticus pugetii, for these bacteria; strain PS-1 is the type strain of C. pugetii.
424 citations
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TL;DR: The results show that bacteria can couple the anaerobic oxidation of methane to denitrification without the involvement of Archaea.
Abstract: Recently, a microbial consortium was shown to couple the anaerobic oxidation of methane to denitrification, predominantly in the form of nitrite reduction to dinitrogen gas. This consortium was dominated by bacteria of an as yet uncharacterized division and archaea of the order Methanosarcinales. The present manuscript reports on the upscaling of the enrichment culture, and addresses the role of the archaea in methane oxidation. The key gene of methanotrophic and methanogenic archaea, mcrA, was sequenced. The associated cofactor F(430) was shown to have a mass of 905 Da, the same as for methanogens and different from the heavier form (951 Da) found in methanotrophic archaea. After prolonged enrichment (> 1 year), no inhibition of anaerobic methane oxidation was observed in the presence of 20 mM bromoethane sulfonate, a specific inhibitor of MCR. Optimization of the cultivation conditions led to higher rates of methane oxidation and to the decline of the archaeal population, as shown by fluorescence in situ hybridization and quantitative MALDI-TOF analysis of F(430). Mass balancing showed that methane oxidation was still coupled to nitrite reduction in the total absence of oxygen. Together, our results show that bacteria can couple the anaerobic oxidation of methane to denitrification without the involvement of Archaea.
423 citations
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TL;DR: Results show that the in vivo protective capacity of the studied lactic acid bacteria (LAB) could be predicted based on the cytokine profile established in vitro, and the PBMC-based assay used may serve as a useful primary indicator to narrow down the number of candidate strains to be tested in murine models for their anti-inflammatory potential.
Abstract: Correlation between in vitro and in vivo immunomodulatory properties of lactic acid bacteria
422 citations